Mouse IGSF8/CD316 Biotinylated Antibody

Catalog #: BAF3117 Datasheet / COA / SDS

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BAF3117

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IGSF8/CD316 in Neuro‑2A Mouse Cell Line.

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Mouse IGSF8/CD316 Biotinylated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse IGSF8/CD316 in Western blots. In Western blots, approximately 10% cross-reactivity with recombinant human IGSF8 is observed and less than 1% cross-reactivity with recombinant mouse IGSF4 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse IGSF8/CD316
Ala25-Thr577
Accession # NP_536344

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse IGSF8

Flow Cytometry

0.25 µg/10⁶ cells

Neuro‑2A mouse neuroblastoma cell line

Immunocytochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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IGSF8/CD316 in Neuro‑2A Mouse Cell Line. IGSF8/CD316 was detected in immersion fixed Neuro-2A mouse neuroblastoma cell line using Goat Anti-Mouse IGSF8/CD316 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF3117) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IGSF8/CD316

IGSF8, also known as PGRL (PG regulatory-like protein), KASP (KAI/CD82 associated protein) and EWI-2 (Glu-Trp-Ile motif 2), is a widely expressed transmembrane adhesion protein. It interacts with beta 1 Integrins and various tetraspanins including CD9, CD81 and CD82. IGSF8 contains four extracellular Ig-like domains. IGSF8 overexpression in transformed cells inhibits cell migration and suppresses cancer metastatic potential. Mouse and human IGSF8 share 90% amino acid sequence identity.

Long Name

Immunoglobulin Superfamily, Member 8

Entrez Gene IDs

93185 (Human); 140559 (Mouse)

Alternate Names

CD316 antigen; CD316; CD81P3; CD81P3LIR-D1; EWI2; EWI-2; EWI2CD81 partner 3; Glu-Trp-Ile EWI motif-containing protein 2; IGSF8; immunoglobulin superfamily, member 8; KCT4; KCT-4; Keratinocytes-associated transmembrane protein 4; PGRL; PGRLimmunoglobulin superfamily member 8