Human/Mouse/Rat IGSF8/CD316 Antibody

Catalog # Availability Size / Price Qty
AF3117
AF3117-SP
Detection of Human and Mouse IGSF8/CD316 by Western Blot.
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Product Details
Citations (6)
FAQs
Supplemental Products
Reviews (1)

Human/Mouse/Rat IGSF8/CD316 Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects mouse IGSF8/CD316 in direct ELISAs. Detects human, mouse, and rat IGSF8/CD316 in Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant human IGSF8 is observed and less than 1% cross-reactivity with recombinant mouse IGSF4 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse IGSF8/CD316
Ala25-Thr577
Accession # NP_536344
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
20 µg/mL
See below
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human and Mouse IGSF8/CD316 antibody by Western Blot. View Larger

Detection of Human and Mouse IGSF8/CD316 by Western Blot. Western blot shows lysates of SH-SY5Y human neuroblastoma cell line, DU145 human prostate carcinoma cell line, and bEnd.3 mouse endothelioma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat IGSF8/CD316 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3117) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for IGSF8/CD316 at approximately 70-80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Western Blot Detection of Rat IGSF8/CD316 antibody by Western Blot. View Larger

Detection of Rat IGSF8/CD316 by Western Blot. Western blot shows lysates of rat brain (hippocampus) tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat IGSF8/CD316 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3117) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for IGSF8/CD316 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Flow Cytometry Detection of IGSF8/CD316 antibody in Neuro-2A Mouse Cell Line antibody by Flow Cytometry. View Larger

Detection of IGSF8/CD316 in Neuro‑2A Mouse Cell Line by Flow Cytometry. Neuro-2A mouse neuroblastoma cell line was stained with Goat Anti-Human/Mouse/Rat IGSF8/CD316 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3117, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).

Immunocytochemistry IGSF8/CD316 antibody in Neuro-2A Mouse Cell Line by Immunocytochemistry (ICC). View Larger

IGSF8/CD316 in Neuro‑2A Mouse Cell Line. IGSF8/CD316 was detected in immersion fixed Neuro-2A mouse neuroblastoma cell line using Goat Anti-Human/Mouse/Rat IGSF8/CD316 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3117) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Simple Western Detection of Human, Mouse, and Rat IGSF8/CD316 antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Human, Mouse, and Rat IGSF8/CD316 by Simple WesternTM. Simple Western lane view shows lysates of human cerebellum tissue, human hippocampus tissue, Neuro-2A mouse neuroblastoma cell line, and rat hippocampus tissue, loaded at 0.2 mg/mL. A specific band was detected for IGSF8/CD316 at approximately 71-80 kDa (as indicated) using 20 µg/mL of Goat Anti-Human/Mouse IGSF8/CD316 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3117) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IGSF8/CD316

IGSF8, also known as PGRL (PG Regulatory-Like Protein), KASP (KAI/CD82 Associated Protein) and EWI-2 (Glu-Trp-Ile motif 2), is a widely expressed transmembrane adhesion protein. It interacts with beta 1 Integrins and various tetraspanins including CD9, CD81 and CD82. IGSF8 contains four extracellular Ig-like domains. IGSF8 over-expression in transformed cells inhibits cell migration and suppresses cancer metastatic potential. Mouse and human IGSF8 share 90% amino acid sequence identity.

Long Name
Immunoglobulin Superfamily, Member 8
Entrez Gene IDs
93185 (Human); 140559 (Mouse)
Alternate Names
CD316 antigen; CD316; CD81P3; CD81P3LIR-D1; EWI2; EWI-2; EWI2CD81 partner 3; Glu-Trp-Ile EWI motif-containing protein 2; IGSF8; immunoglobulin superfamily, member 8; KCT4; KCT-4; Keratinocytes-associated transmembrane protein 4; PGRL; PGRLimmunoglobulin superfamily member 8

Product Datasheets

Citations for Human/Mouse/Rat IGSF8/CD316 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. A conserved sequence in the small intracellular loop of tetraspanins forms an M-shaped inter-helix turn
    Authors: N Reppert, T Lang
    Scientific Reports, 2022;12(1):4494.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Hspa8 and ICAM-1 as damage-induced mediators of &gamma&delta T cell activation
    Authors: MD Johnson, MF Otuki, DA Cabrini, R Rudolph, DA Witherden, WL Havran
    Journal of leukocyte biology, 2021;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Synapse type-specific proteomic dissection identifies IgSF8 as a hippocampal CA3 microcircuit organizer
    Authors: N Apóstolo, SN Smukowski, J Vanderlind, G Condomitti, V Rybakin, J Ten Bos, L Trobiani, S Portegies, KM Vennekens, NV Gounko, D Comoletti, KD Wierda, JN Savas, J de Wit
    Nat Commun, 2020;11(1):5171.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  4. EWI-2 negatively regulates TGF-beta signaling leading to altered melanoma growth and metastasis.
    Authors: Wang H, Sharma C, Knoblich K, Granter S, Hemler M
    Cell Res, 2015;25(3):370-85.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  5. Transcriptome analysis reveals transmembrane targets on transplantable midbrain dopamine progenitors.
    Authors: Bye C, Jonsson M, Bjorklund A, Parish C, Thompson L
    Proc Natl Acad Sci U S A, 2015;112(15):E1946-55.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  6. IgSF8: a developmentally and functionally regulated cell adhesion molecule in olfactory sensory neuron axons and synapses.
    Authors: Ray A, Treloar H
    Mol Cell Neurosci, 2012;50(3):238-49.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation

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Reviews for Human/Mouse/Rat IGSF8/CD316 Antibody

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Human/Mouse/Rat IGSF8/CD316 Antibody
By Anonymous on 01/30/2020
Application: ICC/IF Sample Tested: fixed frozen tissue Species: Mouse

Immunofluorescence experiment on mouse ovary. Was incubated with the primary antibody overnight at room temperature