Mouse IL-1 alpha/IL-1F1 DuoSet ELISA

Catalog # Availability Size / Price Qty
DY400
DY400-05
Ancillary Products Available
IL-1 alpha/IL-1F1 ELISA Kit ELISA
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Product Details
Procedure
Citations (22)
FAQs
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Mouse IL-1 alpha/IL-1F1 DuoSet ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Volume Required
100 µL
Sufficient Materials
For five or fifteen 96-well plates*
Specificity
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant mouse IL-1alpha/IL-1F1. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Kit Content

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.

The components listed above may be purchased separately:

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or 0.05% Tween® 20 in PBS, pH 7.2-7.4

Reagent Diluent: (Catalog # DY995), or 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990)

Plate Sealers: ELISA Plate Sealers (Catalog # DY992)

Data Example

Mouse IL-1 alpha / IL-1F1 ELISA Standard Curve

Product Datasheets

Preparation and Storage

Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-1 alpha/IL-1F1

IL-1 alpha (Interleukin-1 alpha) is a cytokine that is induced during microbial exposure and promotes the release of multiple inflammatory mediators and acute phase proteins. It also supports hematopoietic stem cell expansion, promotes neutrophil mobilization from the bone marrow, and promotes osteoclast formation. IL-1 alpha signals through a receptor complex composed of IL-1 RI and IL-1 RAcP. It also binds the decoy receptor IL-1 RII which associates with IL-1 RAcP but does not transduce signaling.

Long Name:
Interleukin 1 alpha
Entrez Gene IDs:
3552 (Human); 16175 (Mouse); 24493 (Rat); 397094 (Porcine)
Alternate Names:
Hematopoietin-1; IL1 alpha; IL-1 alpha; IL1; IL1A; IL-1A; IL1-ALPHA; IL-1F1; IL1F1hematopoietin-1; interleukin 1, alpha; interleukin-1 alpha; preinterleukin 1 alpha; pro-interleukin-1-alpha

Assay Procedure

GENERAL ELISA PROTOCOL

Plate Preparation

  1. Dilute the Capture Antibody to the working concentration in PBS without carrier protein. Immediately coat a 96-well microplate with 100 μL per well of the diluted Capture Antibody. Seal the plate and incubate overnight at room temperature.
  2. Aspirate each well and wash with Wash Buffer, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (400 μL) using a squirt bottle, manifold dispenser, or autowasher. Complete removal of liquid at each step is essential for good performance. After the last wash, remove any remaining Wash Buffer by aspirating or by inverting the plate and blotting it against clean paper towels.
  3. Block plates by adding 300 μL Reagent Diluent to each well. Incubate at room temperature for a minimum of 1 hour.
  4. Repeat the aspiration/wash as in step 2. The plates are now ready for sample addition.

Assay Procedure

  1. Add 100 μL of sample or standards in Reagent Diluent, or an appropriate diluent, per well. Cover with an adhesive strip and incubate 2 hours at room temperature.
  2. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  3. Add 100 μL of the Detection Antibody, diluted in Reagent Diluent, to each well. Cover with a new adhesive strip and incubate 2 hours at room temperature.
  4. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  5. Add 100 μL of the working dilution of Streptavidin-HRP to each well. Cover the plate and incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  6. Repeat the aspiration/wash as in step 2.
  7. Add 100 μL of Substrate Solution to each well. Incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  8. Add 50 μL of Stop Solution to each well. Gently tap the plate to ensure thorough mixing.
  9. Determine the optical density of each well immediately, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. If wavelength correction is not available, subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.

Citations for Mouse IL-1 alpha/IL-1F1 DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

22 Citations: Showing 1 - 10
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  1. Mycobacterium tuberculosis carrying a rifampicin drug resistance mutation reprograms macrophage metabolism through cell wall lipid changes
    Authors: NC Howard, ND Marin, M Ahmed, BA Rosa, J Martin, M Bambouskov, A Sergushich, E Loginichev, N Kurepina, J Rangel-Mor, L Chen, BN Kreiswirth, RS Klein, JM Balada-Lla, JB Torrelles, GK Amarasingh, M Mitreva, MN Artyomov, FF Hsu, B Mathema, SA Khader
    Nat Microbiol, 2018;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  2. Human caspase-4 detects tetra-acylated LPS and cytosolic Francisella and functions differently from murine caspase-11
    Authors: B Lagrange, S Benaoudia, P Wallet, F Magnotti, A Provost, F Michal, A Martin, F Di Lorenzo, BF Py, A Molinaro, T Henry
    Nat Commun, 2018;9(1):242.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  3. CRISPR/Cas9 mediated mutation of mouse IL-1? nuclear localisation sequence abolishes expression
    Authors: MJD Daniels, AD Adamson, N Humphreys, D Brough
    Sci Rep, 2017;7(1):17077.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  4. Caspase-2 deficiency accelerates chemically induced liver cancer in mice
    Cell Death Differ, 2016;0(0):.
  5. Reducing hypoxia and inflammation during invasive pulmonary aspergillosis by targeting the Interleukin-1 receptor
    Sci Rep, 2016;6(0):26490.
    Species: Mouse
    Sample Types: Tissue Homogenates
  6. Infection-Mediated Priming of Phagocytes Protects against Lethal Secondary Aspergillus fumigatus Challenge
    Authors: A Savers, O Rasid, M Parlato, M Brock, G Jouvion, B Ryffel, JM Cavaillon, G Eberl, O Ibrahim-Gr
    PLoS ONE, 2016;11(4):e0153829.
  7. A small-molecule inhibitor of the NLRP3 inflammasome for the treatment of inflammatory diseases.
    Authors: Coll R, Robertson A, Chae J, Higgins S, Munoz-Planillo R, Inserra M, Vetter I, Dungan L, Monks B, Stutz A, Croker D, Butler M, Haneklaus M, Sutton C, Nunez G, Latz E, Kastner D, Mills K, Masters S, Schroder K, Cooper M, O'Neill L
    Nat Med, 2015;21(3):248-55.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  8. The Inflammatory Caspases-1 and -11 Mediate the Pathogenesis of Dermatitis in Sharpin-Deficient Mice.
    Authors: Douglas T, Champagne C, Morizot A, Lapointe J, Saleh M
    J Immunol, 2015;195(5):2365-73.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  9. A conservative amino acid mutation in the master regulator FleQ renders Pseudomonas aeruginosa aflagellate.
    Authors: Jain, Ruchi, Kazmierczak, Barbara
    PLoS ONE, 2014;9(5):e97439.
    Species: Mouse
    Sample Types: BALF
  10. Signaling via tumor necrosis factor receptor 1 but not Toll-like receptor 2 contributes significantly to hydrosalpinx development following Chlamydia muridarum infection.
    Authors: Dong X, Liu Y, Chang X, Lei L, Zhong G
    Infect Immun, 2014;82(5):1833-9.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  11. Staphylococcus aureus infection of mice expands a population of memory gammadelta T cells that are protective against subsequent infection.
    Authors: Murphy A, O'Keeffe K, Lalor S, Maher B, Mills K, McLoughlin R
    J Immunol, 2014;192(8):3697-708.
    Species: Mouse
    Sample Types: Peritoneal Lavage
  12. Animal model of respiratory syncytial virus: CD8+ T cells cause a cytokine storm that is chemically tractable by sphingosine-1-phosphate 1 receptor agonist therapy.
    Authors: Walsh K, Teijaro J, Brock L, Fremgen D, Collins P, Rosen H, Oldstone M
    J Virol, 2014;88(11):6281-93.
    Species: Mouse
    Sample Types: BALF
  13. MyD88 signaling regulates both host defense and immunopathogenesis during pneumocystis infection.
    Authors: Bello-Irizarry S, Wang J, Johnston C, Gigliotti F, Wright T
    J Immunol, 2014;192(1):282-92.
    Species: Mouse
    Sample Types: BALF
  14. Dendritic cell IL-1alpha and IL-1beta are polyubiquitinated and degraded by the proteasome.
    Authors: Ainscough J, Frank Gerberick G, Zahedi-Nejad M, Lopez-Castejon G, Brough D, Kimber I, Dearman R
    J Biol Chem, 2014;289(51):35582-92.
    Species: Mouse
    Sample Types: Cell Lysates
  15. Mesenchymal stem cells inhibit cutaneous radiation-induced fibrosis by suppressing chronic inflammation.
    Authors: Horton J, Hudak K, Chung E, White A, Scroggins B, Burkeen J, Citrin D
    Stem Cells, 2013;31(10):2231-41.
    Species: Mouse
    Sample Types: Tissue Homogenates
  16. CD4 T cells promote rather than control tuberculosis in the absence of PD-1-mediated inhibition.
    Authors: Barber DL, Mayer-Barber KD, Feng CG
    J. Immunol., 2011;186(3):1598-607.
    Species: Mouse
    Sample Types: BALF
  17. Endothelial cells are central orchestrators of cytokine amplification during influenza virus infection.
    Authors: Teijaro JR, Walsh KB, Cahalan S, Fremgen DM, Roberts E, Scott F, Martinborough E, Peach R, Oldstone MB, Rosen H
    Cell, 2011;146(6):980-91.
    Species: Mouse
    Sample Types: BALF
  18. MyD88 regulation of Fusarium keratitis is dependent on TLR4 and IL-1R1 but not TLR2.
    Authors: Tarabishy AB, Aldabagh B, Sun Y, Imamura Y, Mukherjee PK, Lass JH, Ghannoum MA, Pearlman E
    J. Immunol., 2008;181(1):593-600.
    Species: Mouse
    Sample Types: Tissue Homogenates
  19. Lack of Toll IL-1R8 exacerbates Th17 cell responses in fungal infection.
    Authors: Bozza S, Zelante T, Moretti S, Bonifazi P, DeLuca A, D'Angelo C, Giovannini G, Garlanda C, Boon L, Bistoni F, Puccetti P, Mantovani A, Romani L
    J. Immunol., 2008;180(6):4022-31.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  20. Caspase-1 contributes to Chlamydia trachomatis-induced upper urogenital tract inflammatory pathologies without affecting the course of infection.
    Authors: Cheng W, Shivshankar P, Li Z, Chen L, Yeh IT, Zhong G
    Infect. Immun., 2007;76(2):515-22.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  21. Interleukin 10 regulates inflammatory cytokine synthesis to protect against lipopolysaccharide-induced abortion and fetal growth restriction in mice.
    Authors: Robertson SA, Care AS, Skinner RJ
    Biol. Reprod., 2007;76(5):738-48.
    Species: Mouse
    Sample Types: Serum
  22. Essential role for IL-10 in resistance to lipopolysaccharide-induced preterm labor in mice.
    Authors: Robertson SA, Skinner RJ, Care AS
    J. Immunol., 2006;177(7):4888-96.
    Species: Mouse
    Sample Types: Serum

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Mouse IL-1 alpha/IL-1F1 DuoSet ELISA, 15 Plate
By Anonymous on 04/08/2016
Sample Tested: murine lung homogenate

very easy to use, consistent results between runs.