Mouse IL-1 alpha/IL-1F1 Quantikine ELISA Kit

R&D Systems | Catalog # MLA00

R&D Systems
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Key Product Details

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 µL), Serum (10 µL)

Sensitivity

2.5 pg/mL

Assay Range

4.69-300 pg/mL (Cell Culture Supernates, Serum)
View all IL-1 alpha/IL-1F1 ELISA Kits »

Product Summary for Mouse IL-1 alpha/IL-1F1 Quantikine ELISA Kit

The Quantikine Mouse IL-1 alpha Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse IL-1 alpha levels in cell culture supernates and serum. It contains E. coli-expressed recombinant mouse IL-1 alpha and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant mouse IL-1 alpha accurately. Results obtained using natural mouse IL-1 alpha showed dose response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse IL-1 alpha.

Product Specifications

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Measurement

Quantitative ELISA

Detection Method

Colorimetric - 450nm (TMB)

Conjugate

HRP

Species

Mouse

Specificity

Natural and recombinant mouse IL-1 alpha

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

Interference observed with 1 or more available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Cell Culture Supernates, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 17 32 156 18 33 162
Standard Deviation 1.5 1.0 3.9 0.9 1.2 7.9
CV% 8.8 3.1 2.5 5.0 3.6 4.9

Recovery for Mouse IL-1 alpha/IL-1F1 Quantikine ELISA Kit

The recovery of mouse IL-1 alpha spiked to three levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Supernates (n=5) 102 92-109
Serum (n=6) 94 88-103

Linearity

To assess the linearity of the assay, five or more samples spiked with various concentrations of mouse IL-1 alpha in each matrix were diluted with Calibrator Diluent and then assayed.

Mouse IL-1 alpha/IL-1F1 ELISA Linearity

Scientific Data Images for Mouse IL-1 alpha/IL-1F1 Quantikine ELISA Kit

Mouse IL-1 alpha/IL-1F1 ELISA Standard Curve

Mouse IL-1 alpha/IL-1F1 ELISA Standard Curve

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-1 alpha/IL-1F1

IL-1 alpha (Interleukin-1 alpha) is a cytokine that is induced during microbial exposure and promotes the release of multiple inflammatory mediators and acute phase proteins. It also supports hematopoietic stem cell expansion, promotes neutrophil mobilization from the bone marrow, and promotes osteoclast formation. IL-1 alpha signals through a receptor complex composed of IL-1 RI and IL-1 RAcP. It also binds the decoy receptor IL-1 RII which associates with IL-1 RAcP but does not transduce signalling.

 

Long Name

Interleukin 1 alpha

Alternate Names

BAF, IL-1A, IL-1F1, IL1, IL1 alpha, IL1A, IL1F1, LAF, LEM

Entrez Gene IDs

3552 (Human); 16175 (Mouse); 24493 (Rat); 397094 (Porcine); 101926546 (Cynomolgus Monkey)

Gene Symbol

IL1A

Additional IL-1 alpha/IL-1F1 Products

Product Documents for Mouse IL-1 alpha/IL-1F1 Quantikine ELISA Kit

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse IL-1 alpha/IL-1F1 Quantikine ELISA Kit

For research use only

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Citations for Mouse IL-1 alpha/IL-1F1 Quantikine ELISA Kit

Customer Reviews for Mouse IL-1 alpha/IL-1F1 Quantikine ELISA Kit (6)

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  • Mouse IL-1 alpha/IL-1F1 Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: bone marrow
    Verified Customer | Posted 12/22/2021
    5 times dilution
    Mouse IL-1 alpha/IL-1F1 Quantikine ELISA Kit MLA00
  • Mouse IL-1 alpha/IL-1F1 Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: moues cell line conditioned media
    Verified Customer | Posted 12/21/2021
  • Mouse IL-1 alpha/IL-1F1 Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: EDTA Plasma and Bone marrow lavage
    Verified Customer | Posted 05/21/2021
    Mouse IL-1 alpha/IL-1F1 Quantikine ELISA Kit MLA00
  • Mouse IL-1 alpha/IL-1F1 Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: Serum
    Verified Customer | Posted 08/06/2018
  • Mouse IL-1 alpha/IL-1F1 Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: Pancreatic cancer cells and Adipocytes
    Verified Customer | Posted 04/11/2018
  • Name: Anonymous
    Sample Tested: mouse pancreatic cell line
    Verified Customer | Posted 03/30/2018

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Protocols

View specific protocols for Mouse IL-1 alpha/IL-1F1 Quantikine ELISA Kit (MLA00):

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
    3. 50 µL Assay Diluent
  3.   Add 50 µL of Assay Diluent to each well.
    4. 50 µL Standard, Control, or Sample
  4.   Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  5.   Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.
    6. 100 µL Conjugate
  6.   Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
  7.   Aspirate and wash 5 times.
    8. 100 µL Substrate Solution
  8.   Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.
    9. 100 µL Stop Solution
  9.   Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

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FAQs

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Associated Pathways

IL-1 Family Signaling Pathways IL-1 Family Signaling Pathway Thumbnail