Mouse IL-1 alpha/IL-1F1 Quantikine ELISA Kit

Catalog # Availability Size / Price Qty

15% Off Limited-time Savings on Your Reagents, Assays, and Kits
Now through September 27th, you can save 15% on Bio-Techne reagents, assays, and kits (including Novus, Tocris, and R&D Systems). Use promotion code SAVE15 at checkout on orders over $1,000. See terms and conditions for offer details.

Mouse IL-1 alpha/IL-1F1 ELISA Standard Curve
2 Images
Product Details
Citations (30)
Supplemental Products
Reviews (6)

Mouse IL-1 alpha/IL-1F1 Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (10 uL)
2.5 pg/mL
Assay Range
4.7 - 300 pg/mL (Cell Culture Supernates, Serum)
Natural and recombinant mouse IL-1 alpha
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference observed with 1 or more available related molecules.

Product Summary

The Quantikine Mouse IL-1 alpha Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse IL-1 alpha levels in cell culture supernates and serum. It contains E. coli-expressed recombinant mouse IL-1 alpha and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant mouse IL-1 alpha accurately. Results obtained using natural mouse IL-1 alpha showed dose response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse IL-1 alpha.


Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 17 32 156 18 33 162
Standard Deviation 1.5 1 3.9 0.9 1.2 7.9
CV% 8.8 3.1 2.5 5 3.6 4.9


The recovery of mouse IL-1 alpha spiked to three levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Supernates (n=5) 102 92-109
Serum (n=6) 94 88-103


To assess the linearity of the assay, five or more samples spiked with various concentrations of mouse IL-1 alpha in each matrix were diluted with Calibrator Diluent and then assayed.
Mouse IL-1 alpha/IL-1F1 ELISA Linearity

Data Examples

Mouse IL-1 alpha/IL-1F1 ELISA Standard Curve

Product Datasheets

You must select a language.


Preparation and Storage

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-1 alpha/IL-1F1

IL-1 alpha (Interleukin-1 alpha) is a cytokine that is induced during microbial exposure and promotes the release of multiple inflammatory mediators and acute phase proteins. It also supports hematopoietic stem cell expansion, promotes neutrophil mobilization from the bone marrow, and promotes osteoclast formation. IL-1 alpha signals through a receptor complex composed of IL-1 RI and IL-1 RAcP. It also binds the decoy receptor IL-1 RII which associates with IL-1 RAcP but does not transduce signaling.

Long Name:
Interleukin 1 alpha
Entrez Gene IDs:
3552 (Human); 16175 (Mouse); 24493 (Rat); 397094 (Porcine); 101926546 (Cynomolgus Monkey)
Alternate Names:
BAF; Hematopoietin-1; IL1 alpha; IL-1 alpha; IL1; IL1A; IL-1A; IL1-ALPHA; IL1F1; IL-1F1; IL1F1hematopoietin-1; interleukin 1, alpha; interleukin-1 alpha; LAF; LEM; preinterleukin 1 alpha; pro-interleukin-1-alpha
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 50 µL Assay Diluent
  4.   Add 50 µL of Assay Diluent to each well.

  5. 50 µL Standard, Control, or Sample
  6.   Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  7.   Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.

  8. 100 µL Conjugate
  9.   Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
  10.   Aspirate and wash 5 times.

  11. 100 µL Substrate Solution
  12.   Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

  13. 100 µL Stop Solution
  14.   Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Mouse IL-1 alpha/IL-1F1 Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

30 Citations: Showing 1 - 10
Filter your results:

Filter by:

  1. Targeting necroptosis in muscle fibers ameliorates inflammatory myopathies
    Authors: M Kamiya, F Mizoguchi, K Kawahata, D Wang, M Nishibori, J Day, C Louis, IP Wicks, H Kohsaka, S Yasuda
    Nature Communications, 2022;13(1):166.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  2. Augmented secretion of IL-1&alpha from mouse oral squamous cell carcinoma (OSCC) cells caused by serum deprivation and hypoxia promotes immune suppressive activity of mesenchymal stromal cells
    Authors: A Matsunami, M Mizuno-Kam, H Kawaki, E Takayama, K Ueno, M Ando, H Morimoto-I, Y Muramatsu, S Sumitomo, N Kondoh
    Journal of oral biosciences, 2021;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  3. Intralesional injection of rose bengal augments the efficacy of gemcitabine chemotherapy against pancreatic tumors
    Authors: P Innamarato, J Morse, A Mackay, S Asby, M Beatty, J Blauvelt, S Kidd, JE Mullinax, AA Sarnaik, S Pilon-Thom
    BMC Cancer, 2021;21(1):756.
    Species: Mouse
    Sample Types: Serum
  4. Type 1 interferon mediates chronic stress-induced neuroinflammation and behavioral deficits via complement component 3-dependent pathway
    Authors: A Tripathi, C Whitehead, K Surrao, A Pillai, A Madeshiya, Y Li, H Khodadadi, AO Ahmed, G Turecki, B Baban, A Pillai
    Molecular Psychiatry, 2021;0(0):.
    Species: Mouse
    Sample Types: Serum
  5. Pancreatic, but not myeloid-cell, expression of interleukin-1alpha is required for maintenance of insulin secretion and whole body glucose homeostasis
    Authors: JJ Collier, HM Batdorf, TM Martin, KE Rohli, DH Burk, D Lu, CR Cooley, MD Karlstad, JW Jackson, TE Sparer, J Zhang, RL Mynatt, SJ Burke
    Molecular Metabolism, 2020;44(0):101140.
    Species: Mouse
    Sample Types: Cell Lysates
  6. Impact of combined ultrasound-microwave treatment on structural and functional properties of golden threadfin bream (Nemipterus virgatus) myofibrillar proteins and hydrolysates
    Authors: Z Li, J Wang, B Zheng, Z Guo
    Ultrason Sonochem, 2020;65(0):105063.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  7. A dual inhibitor of the proteasome catalytic subunits LMP2 and Y attenuates disease progression in mouse models of Alzheimer's disease
    Authors: IJ Yeo, MJ Lee, A Baek, Z Miller, D Bhattarai, YM Baek, HJ Jeong, YK Kim, DE Kim, JT Hong, KB Kim
    Sci Rep, 2019;9(1):18393.
    Species: Mouse
    Sample Types: Serum
  8. Pentosan Polysulfate Treatment of Mucopolysaccharidosis Type IIIA Mice
    Authors: N Guo, V DeAngelis, C Zhu, EH Schuchman, CM Simonaro
    JIMD Rep, 2018;0(0):.
    Species: Mouse
    Sample Types: Plasma
  9. Specific calpain inhibition protects kidney against inflammaging
    Authors: G Hanouna, L Mesnard, S Vandermeer, J Perez, S Placier, JP Haymann, F Campagne, J Moroch, A Bataille, L Baud, E Letavernie
    Sci Rep, 2017;7(1):8016.
    Species: Mouse
    Sample Types: Peritoneal Fluid
  10. Kruppel-like factor 6 promotes macrophage-mediated inflammation by suppressing B cell leukemia/lymphoma 6 expression
    J Biol Chem, 2016;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  11. RIP3-mediated necrotic cell death accelerates systematic inflammation and mortality.
    Authors: Meng L, Jin W, Wang X
    Proc Natl Acad Sci U S A, 2015;112(35):11007-12.
  12. The senescence-associated secretory phenotype promotes benign prostatic hyperplasia.
    Authors: Vital P, Castro P, Tsang S, Ittmann M
    Am J Pathol, 2014;184(3):721-31.
    Species: Mouse
    Sample Types: Tissue Homogenates
  13. Pivotal role of phospholipase D1 in tumor necrosis factor-alpha-mediated inflammation and scar formation after myocardial ischemia and reperfusion in mice.
    Authors: Schonberger T, Jurgens T, Muller J, Armbruster N, Niermann C, Gorressen S, Sommer J, Tian H, di Paolo G, Scheller J, Fischer J, Gawaz M, Elvers M
    Am J Pathol, 2014;184(9):2450-64.
    Species: Mouse
    Sample Types: Plasma
  14. The effects of protein malnutrition on the TNF-RI and NF-kappaB expression via the TNF-alpha signaling pathway.
    Authors: de Oliveira D, Hastreiter A, Mello A, de Oliveira Beltran J, Oliveira Santos E, Borelli P, Fock R
    Cytokine, 2014;69(2):218-25.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  15. Running exercise alleviates trabecular bone loss and osteopenia in hemizygous beta-globin knockout thalassemic mice.
    Authors: Thongchote K, Svasti S, Teerapornpuntakit J, Krishnamra N, Charoenphandhu N
    Am J Physiol Endocrinol Metab, 2014;306(12):E1406-17.
    Species: Mouse
    Sample Types: Serum
  16. Elevating body temperature enhances hematopoiesis and neutrophil recovery after total body irradiation in an IL-1-, IL-17-, and G-CSF-dependent manner.
    Blood, 2012;120(13):2600-9.
    Species: Mouse
    Sample Types: Tissue Homogenates
  17. Tissue-specific opposing functions of the inflammasome adaptor ASC in the regulation of epithelial skin carcinogenesis.
    Authors: Drexler, Stefan K, Bonsignore, Luca, Masin, Mark, Tardivel, Aubry, Jackstadt, Rene, Hermeking, Heiko, Schneider, Pascal, Gross, Olaf, Tschopp, Jurg, Yazdi, Amir S
    Proc Natl Acad Sci U S A, 2012;109(45):18384-9.
    Species: Mouse
    Sample Types: Tissue Homogenates
  18. Role of IL-1alpha and the Nlrp3/caspase-1/IL-1beta axis in cigarette smoke-induced pulmonary inflammation and COPD.
    Authors: Pauwels NS, Bracke KR, Dupont LL, Van Pottelberge GR, Provoost S, Vanden Berghe T, Vandenabeele P, Lambrecht BN, Joos GF, Brusselle GG
    Eur. Respir. J., 2011;38(5):1019-28.
    Species: Mouse
    Sample Types: BALF
  19. Sphingosine regulates the NLRP3-inflammasome and IL-1beta release from macrophages.
    Authors: Luheshi NM, Giles JA, Lopez-Castejon G, Brough D
    Eur. J. Immunol., 2011;42(3):716-25.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  20. Histone Deacetylase Inhibitor LAQ824 Augments Inflammatory Responses in Macrophages through Transcriptional Regulation of IL-10.
    Authors: Wang H, Cheng F
    J. Immunol., 2011;186(0):3986.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  21. Activation of the NLRP3 inflammasome by islet amyloid polypeptide provides a mechanism for enhanced IL-1beta in type 2 diabetes.
    Authors: Masters SL, Dunne A, Subramanian SL, Hull RL, Tannahill GM, Sharp FA, Becker C, Franchi L, Yoshihara E, Chen Z, Mullooly N, Mielke LA, Harris J, Coll RC, Mills KH, Mok KH, Newsholme P, Nunez G, Yodoi J, Kahn SE, Lavelle EC, O'Neill LA
    Nat. Immunol., 2010;11(10):897-904.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  22. Varying susceptibility of pulmonary cytokine production to lipopolysaccharide in mice.
    Authors: Alm AS, Li K, Yang D, Andersson R, Lu Y, Wang X
    Cytokine, 2010;49(3):256-63.
    Species: Mouse
    Sample Types: BALF
  23. Tumor necrosis factor-alpha and interleukin-1 antagonists alleviate inflammatory skin changes associated with epidermal growth factor receptor antibody therapy in mice.
    Authors: Surguladze D, Deevi D, Claros N, Corcoran E, Wang S, Plym MJ, Wu Y, Doody J, Mauro DJ, Witte L, Busam KJ, Pytowski B, Rodeck U, Tonra JR
    Cancer Res., 2009;69(14):5643-7.
    Species: Mouse
    Sample Types: Tissue Homogenates
  24. A MyD88-dependent early IL-17 production protects mice against airway infection with the obligate intracellular pathogen Chlamydia muridarum.
    Authors: Zhang X, Gao L, Lei L, Zhong Y, Dube P, Berton MT, Arulanandam B, Zhang J, Zhong G
    J. Immunol., 2009;183(2):1291-300.
    Species: Mouse
    Sample Types: BALF
  25. Viral infection of the lungs through the eye.
    Authors: Bitko V, Musiyenko A, Barik S
    J. Virol., 2007;81(2):783-90.
    Species: Mouse
    Sample Types: Tissue Homogenates
  26. Absence of IL-1 receptor antagonist impaired wound healing along with aberrant NF-kappaB activation and a reciprocal suppression of TGF-beta signal pathway.
    Authors: Ishida Y, Kondo T, Kimura A, Matsushima K, Mukaida N
    J. Immunol., 2006;176(9):5598-606.
    Species: Mouse
    Sample Types: Tissue Homogenates
  27. IL-1 mediates TNF-induced osteoclastogenesis.
    Authors: Wei S, Kitaura H, Zhou P, Ross FP, Teitelbaum SL
    J. Clin. Invest., 2005;115(2):282-90.
    Species: Mouse
    Sample Types: Cell Lysates
  28. Increased interleukin-10 associated with low IL-6 concentration correlated with greater survival rates in mice infected by rabies virus vaccinated against it and immunomodulated with P. acnes.
    Authors: Megid J, Kaneno R, Nozaki CN, Brito CJ, Almeida MF
    Comp. Immunol. Microbiol. Infect. Dis., 2004;27(6):393-411.
    Species: Mouse
    Sample Types: Serum
  29. T cell-specific ablation of Fas leads to Fas ligand-mediated lymphocyte depletion and inflammatory pulmonary fibrosis.
    Authors: Hao Z, Hampel B, Yagita H, Rajewsky K
    J. Exp. Med., 2004;199(10):1355-65.
    Species: Mouse
    Sample Types: Serum
  30. The protein tyrosine kinase inhibitor AG126 prevents the massive microglial cytokine induction by pneumococcal cell walls.
    Authors: Hanisch UK, Prinz M, Angstwurm K, Hausler KG, Kann O, Kettenmann H, Weber JR
    Eur. J. Immunol., 2001;31(7):2104-15.
    Species: Mouse
    Sample Types: Cell Culture Supernates


No product specific FAQs exist for this product, however you may

View all ELISA FAQs

Supplemental ELISA Products

Reviews for Mouse IL-1 alpha/IL-1F1 Quantikine ELISA Kit

Average Rating: 4.8 (Based on 6 Reviews)

5 Star
4 Star
3 Star
2 Star
1 Star

Have you used Mouse IL-1 alpha/IL-1F1 Quantikine ELISA Kit?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:

Mouse IL-1 alpha/IL-1F1 Quantikine ELISA Kit
By Anonymous on 12/22/2021
Sample Tested: bone marrow

5 times dilution

Mouse IL-1 alpha/IL-1F1 Quantikine ELISA Kit
By Anonymous on 12/21/2021
Sample Tested: moues cell line conditioned media

Mouse IL-1 alpha/IL-1F1 Quantikine ELISA Kit
By Anonymous on 05/21/2021
Sample Tested: Bone marrow lavage,EDTA Plasma

Mouse IL-1 alpha/IL-1F1 Quantikine ELISA Kit
By Anonymous on 08/06/2018
Sample Tested: Serum

Mouse IL-1 alpha/IL-1F1 Quantikine ELISA Kit
By Anonymous on 04/11/2018
Sample Tested: Adipocytes,Pancreatic cancer cells

Mouse IL-1 alpha/IL-1F1 Quantikine ELISA Kit
By Anonymous on 03/30/2018
Sample Tested: mouse pancreatic cell line