Mouse IL-1 alpha/IL-1F1 Quantikine ELISA Kit

(22 citations)   
  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (50 uL), Serum (10 uL)
  • Sensitivity
    2.5 pg/mL
  • Assay Range
    4.7 - 300 pg/mL (Cell Culture Supernates, Serum)
  • Specificity
    Natural and recombinant mouse IL-1 alpha
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    Interference observed with 1 or more available related molecules.
Product Summary
The Quantikine Mouse IL-1 alpha Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse IL-1 alpha levels in cell culture supernates and serum. It contains E. coli-expressed recombinant mouse IL-1 alpha and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant mouse IL-1 alpha accurately. Results obtained using natural mouse IL-1 alpha showed dose response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse IL-1 alpha.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum
Intra-Assay Precision Inter-Assay Precision
Sample123123
n202020202020
Mean17321561833162
Standard Deviation1.513.90.91.27.9
CV%8.83.12.553.64.9

Recovery

The recovery of mouse IL-1 alpha spiked to three levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Supernates (n=5) 102 92-109
Serum (n=6) 94 88-103
Linearity
To assess the linearity of the assay, five or more samples spiked with various concentrations of mouse IL-1 alpha in each matrix were diluted with Calibrator Diluent and then assayed.
Mouse IL-1 alpha/IL-1F1 Quantikine ELISA Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: IL-1 alpha/IL-1F1
IL-1 alpha (Interleukin-1 alpha) is a cytokine that is induced during microbial exposure and promotes the release of multiple inflammatory mediators and acute phase proteins. It also supports hematopoietic stem cell expansion, promotes neutrophil mobilization from the bone marrow, and promotes osteoclast formation. IL-1 alpha signals through a receptor complex composed of IL-1 RI and IL-1 RAcP. It also binds the decoy receptor IL-1 RII which associates with IL-1 RAcP but does not transduce signaling.
    • Long Name
      Interleukin 1 alpha
    • Entrez Gene IDs
      3552 (Human); 16175 (Mouse); 24493 (Rat); 397094 (Porcine);
    • Alternate Names
      Hematopoietin-1; IL1 alpha; IL-1 alpha; IL1; IL1A; IL-1A; IL1-ALPHA; IL-1F1; IL1F1hematopoietin-1; interleukin 1, alpha; interleukin-1 alpha; preinterleukin 1 alpha; pro-interleukin-1-alpha;
    Related Research Areas
    Assay Procedure
    Refer to the product for complete assay procedure.

    Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
    1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
    2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

    3. 50 µL Assay Diluent
    4.   Add 50 µL of Assay Diluent to each well.

    5. 50 µL Standard, Control, or Sample
    6.   Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
    7.   Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.

    8. 100 µL Conjugate
    9.   Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
    10.   Aspirate and wash 5 times.

    11. 100 µL Substrate Solution
    12.   Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

    13. 100 µL Stop Solution
    14.   Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
    Citations:

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    22 Citations: Showing 1 - 10
    Filter your results:

    Species
    Sample Type
    1. Specific calpain inhibition protects kidney against inflammaging
      Authors: G Hanouna, L Mesnard, S Vandermeer, J Perez, S Placier, JP Haymann, F Campagne, J Moroch, A Bataille, L Baud, E Letavernie
      Sci Rep, 2017;7(1):8016.
      Species: Mouse
      Sample Type: Peritoneal Fluid
    2. Kruppel-like factor 6 promotes macrophage-mediated inflammation by suppressing B cell leukemia/lymphoma 6 expression
      J Biol Chem, 2016;0(0):.
      Species: Mouse
      Sample Type: Tissue Homogenates
    3. RIP3-mediated necrotic cell death accelerates systematic inflammation and mortality.
      Authors: Meng L, Jin W, Wang X
      Proc Natl Acad Sci U S A, 2015;112(35):11007-12.
    4. The senescence-associated secretory phenotype promotes benign prostatic hyperplasia.
      Authors: Vital P, Castro P, Tsang S, Ittmann M
      Am J Pathol, 2014;184(3):721-31.
      Species: Mouse
      Sample Type: Tissue Homogenates
    5. Running exercise alleviates trabecular bone loss and osteopenia in hemizygous beta-globin knockout thalassemic mice.
      Authors: Thongchote K, Svasti S, Teerapornpuntakit J, Krishnamra N, Charoenphandhu N
      Am J Physiol Endocrinol Metab, 2014;306(12):E1406-17.
      Species: Mouse
      Sample Type: Serum
    6. The effects of protein malnutrition on the TNF-RI and NF-kappaB expression via the TNF-alpha signaling pathway.
      Authors: de Oliveira D, Hastreiter A, Mello A, de Oliveira Beltran J, Oliveira Santos E, Borelli P, Fock R
      Cytokine, 2014;69(2):218-25.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    7. Pivotal role of phospholipase D1 in tumor necrosis factor-alpha-mediated inflammation and scar formation after myocardial ischemia and reperfusion in mice.
      Authors: Schonberger T, Jurgens T, Muller J, Armbruster N, Niermann C, Gorressen S, Sommer J, Tian H, di Paolo G, Scheller J, Fischer J, Gawaz M, Elvers M
      Am J Pathol, 2014;184(9):2450-64.
      Species: Mouse
      Sample Type: Plasma
    8. Tissue-specific opposing functions of the inflammasome adaptor ASC in the regulation of epithelial skin carcinogenesis.
      Authors: Drexler, Stefan K, Bonsignore, Luca, Masin, Mark, Tardivel, Aubry, Jackstadt, Rene, Hermeking, Heiko, Schneider, Pascal, Gross, Olaf, Tschopp, Jurg, Yazdi, Amir S
      Proc Natl Acad Sci U S A, 2012;109(45):18384-9.
      Species: Mouse
      Sample Type: Tissue Homogenates
    9. Elevating body temperature enhances hematopoiesis and neutrophil recovery after total body irradiation in an IL-1-, IL-17-, and G-CSF-dependent manner.
      Blood, 2012;120(13):2600-9.
      Species: Mouse
      Sample Type: Tissue Homogenates
    10. Sphingosine regulates the NLRP3-inflammasome and IL-1beta release from macrophages.
      Authors: Luheshi NM, Giles JA, Lopez-Castejon G, Brough D
      Eur. J. Immunol., 2011;42(3):716-25.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    11. Histone Deacetylase Inhibitor LAQ824 Augments Inflammatory Responses in Macrophages through Transcriptional Regulation of IL-10.
      Authors: Wang H, Cheng F
      J. Immunol., 2011;186(0):3986.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    12. Role of IL-1alpha and the Nlrp3/caspase-1/IL-1beta axis in cigarette smoke-induced pulmonary inflammation and COPD.
      Authors: Pauwels NS, Bracke KR, Dupont LL, Van Pottelberge GR, Provoost S, Vanden Berghe T, Vandenabeele P, Lambrecht BN, Joos GF, Brusselle GG
      Eur. Respir. J., 2011;38(5):1019-28.
      Species: Mouse
      Sample Type: BALF
    13. Activation of the NLRP3 inflammasome by islet amyloid polypeptide provides a mechanism for enhanced IL-1beta in type 2 diabetes.
      Authors: Masters SL, Dunne A, Subramanian SL, Hull RL, Tannahill GM, Sharp FA, Becker C, Franchi L, Yoshihara E, Chen Z, Mullooly N, Mielke LA, Harris J, Coll RC, Mills KH, Mok KH, Newsholme P, Nunez G, Yodoi J, Kahn SE, Lavelle EC, O'Neill LA
      Nat. Immunol., 2010;11(10):897-904.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    14. Varying susceptibility of pulmonary cytokine production to lipopolysaccharide in mice.
      Authors: Alm AS, Li K, Yang D, Andersson R, Lu Y, Wang X
      Cytokine, 2010;49(3):256-63.
      Species: Mouse
      Sample Type: BALF
    15. A MyD88-dependent early IL-17 production protects mice against airway infection with the obligate intracellular pathogen Chlamydia muridarum.
      Authors: Zhang X, Gao L, Lei L, Zhong Y, Dube P, Berton MT, Arulanandam B, Zhang J, Zhong G
      J. Immunol., 2009;183(2):1291-300.
      Species: Mouse
      Sample Type: BALF
    16. Tumor necrosis factor-alpha and interleukin-1 antagonists alleviate inflammatory skin changes associated with epidermal growth factor receptor antibody therapy in mice.
      Authors: Surguladze D, Deevi D, Claros N, Corcoran E, Wang S, Plym MJ, Wu Y, Doody J, Mauro DJ, Witte L, Busam KJ, Pytowski B, Rodeck U, Tonra JR
      Cancer Res., 2009;69(14):5643-7.
      Species: Mouse
      Sample Type: Tissue Homogenates
    17. Viral infection of the lungs through the eye.
      Authors: Bitko V, Musiyenko A, Barik S
      J. Virol., 2007;81(2):783-90.
      Species: Mouse
      Sample Type: Tissue Homogenates
    18. Absence of IL-1 receptor antagonist impaired wound healing along with aberrant NF-kappaB activation and a reciprocal suppression of TGF-beta signal pathway.
      Authors: Ishida Y, Kondo T, Kimura A, Matsushima K, Mukaida N
      J. Immunol., 2006;176(9):5598-606.
      Species: Mouse
      Sample Type: Tissue Homogenates
    19. IL-1 mediates TNF-induced osteoclastogenesis.
      Authors: Wei S, Kitaura H, Zhou P, Ross FP, Teitelbaum SL
      J. Clin. Invest., 2005;115(2):282-90.
      Species: Mouse
      Sample Type: Cell Lysates
    20. T cell-specific ablation of Fas leads to Fas ligand-mediated lymphocyte depletion and inflammatory pulmonary fibrosis.
      Authors: Hao Z, Hampel B, Yagita H, Rajewsky K
      J. Exp. Med., 2004;199(10):1355-65.
      Species: Mouse
      Sample Type: Serum
    21. Increased interleukin-10 associated with low IL-6 concentration correlated with greater survival rates in mice infected by rabies virus vaccinated against it and immunomodulated with P. acnes.
      Authors: Megid J, Kaneno R, Nozaki CN, Brito CJ, Almeida MF
      Comp. Immunol. Microbiol. Infect. Dis., 2004;27(6):393-411.
      Species: Mouse
      Sample Type: Serum
    22. The protein tyrosine kinase inhibitor AG126 prevents the massive microglial cytokine induction by pneumococcal cell walls.
      Authors: Hanisch UK, Prinz M, Angstwurm K, Hausler KG, Kann O, Kettenmann H, Weber JR
      Eur. J. Immunol., 2001;31(7):2104-15.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    Expand to show all 22 Citations
    Supplemental ELISA Products
    Description Application Cat# Citations Images  

    Quantikine ELISA Wash Buffer 1

    ELISA WA126 6
    View Sizes & Prices
    Catalog# Size Price Stock Quantity
    Loading Information....

    Have you used Mouse IL-1 alpha/IL-1F1 Quantikine ELISA Kit?

    Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

    Order Details

    Contact Information

    R&D Systems Guarantee

    Associated Pathways

    Customers Who Viewed
    This Item Also Viewed

    Feedback

    Customer Information