Mouse IL-12/IL-35 p35 Antibody

MAB6688 4 Citations Datasheet / COA / SDS

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MAB6688
MAB6688-SP

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Citations (4)

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Mouse IL-12/IL-35 p35 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse IL-12/IL-35 p35 in direct ELISAs. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse (rm) IL-12 p35+p40 heterodimer or recombinant rat IL-12 p35 is observed. No cross-reactivity with recombinant human IL-12 p35, rmIL-12 p40, rmIL-23 p19+p40 heterodimer, recombinant canine IL-12 p35+p40 heterodimer, or recombinant feline IL-12 p35+p40 heterodimer is observed.

Source

Monoclonal Rat IgG_2A Clone # 45806

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived mouse IL-12/IL-35 p35
Arg23-Ala215
Accession # NP_032377

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Immunohistochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunohistochemistry

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IL‑12/IL-35 p35 in Mouse Spleen. IL-12/IL-35 p35 was detected in immersion fixed frozen sections of mouse spleen using Rat Anti-Mouse IL-12/IL-35 p35 Monoclonal Antibody (Catalog # MAB6688) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hemotoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-12/IL-35 p35

Interleukin 12, also known as Natural Killer Cell Stimulatory Factor (NKSF) or Cytotoxic Lymphocyte Maturation Factor (CLMF), is a heterodimeric pleiotropic cytokine made up of a 40 kDa (p40) subunit and a 35 kDa (p35) subunit. IL‑12 is produced by macrophages and B lymphocytes and has been shown to have multiple effects on T cells and Natural Killer (NK) cells. Some of these IL‑12 activities include the induction of IFN‑ gamma and TNF in resting and activated T and NK cells; the enhancement of cytotoxic activity of resting NK and T cells, the stimulation of resting T cell proliferation in the presence of a comitogen; and the enhancement of NK cell proliferation. IL‑12 is a key mediator of cellular-immunity and induces the differentiation of Th1 cells from precursor T helper cells. Based on its activities, it has been suggested that IL‑12 may have therapeutic potential as a vaccine adjuvant that promotes cellular-immunity and as an anti-tumor and anti-viral agent. Human and mouse IL‑12 share 70% and 60% amino acid sequence identity in their p40 and p35 subunits, respectively.

Long Name

Interleukin 12 p35

Entrez Gene IDs

3592 (Human); 16159 (Mouse); 84405 (Rat)

Alternate Names

IL-12/IL-35 p35; IL-12A; IL35p35

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Mouse IL-12/IL-35 p35 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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IL35 modulation altered survival, cytokine environment and histopathological consequences during malaria infection in mice
Authors: RO Bello, MA Abdullah, R Abd Majid, VK Chin, MF Abd Rachma, ZO Ibraheem, MK Hussain, MG Magaji, R Basir
Malar. J., 2019;18(1):434.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P
Interleukin-12p35 Knock Out Aggravates Doxorubicin-Induced Cardiac Injury and Dysfunction by Aggravating the Inflammatory Response, Oxidative Stress, Apoptosis and Autophagy in Mice
Authors: J Ye, Y Huang, B Que, C Chang, W Liu, H Hu, L Liu, Y Shi, Y Wang, M Wang, T Zeng, W Zhen, Y Xu, L Shi, J Liu, H Jiang, D Ye, Y Lin, J Wan, Q Ji
EBioMedicine, 2018;35(0):29-39.
Species: Human
Sample Types: Whole Tissue
Applications: IHC
Lentiviral delivery of novel fusion protein IL12/FasTI for cancer immune/gene therapy
Authors: X Yang, X Yu, Y Wei
PLoS ONE, 2018;13(7):e0201100.
Species: Human
Sample Types: Whole Cells
Applications: ICC
Prevention of autoimmune diabetes by ectopic pancreatic beta-cell expression of interleukin-35.
Authors: Bettini M, Castellaw AH, Lennon GP, Burton AR, Vignali DA
Diabetes, 2012;61(6):1519-26.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC