Mouse IL-13 DuoSet ELISA

Catalog # Availability Size / Price Qty
DY413
DY413-05
Ancillary Products Available
Mouse IL-13 ELISA Standard Curve
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Product Details
Procedure
Citations (87)
FAQs
Supplemental Products
Reviews (1)

Mouse IL-13 DuoSet ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Volume Required
100 µL
Sufficient Materials
For five or fifteen 96-well plates*
Specificity
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant mouse IL-13. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Kit Content

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.

The components listed above may be purchased separately:

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or 0.05% Tween® 20 in PBS, pH 7.2-7.4

Reagent Diluent: (Catalog # DY995), or 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990)

Plate Sealers: ELISA Plate Sealers (Catalog # DY992)

Product Datasheets

Preparation and Storage

Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-13

IL-13 (Interleukin-13) plays a key role in the pathogenesis of allergy, cancer, and tissue fibrosis. It is secreted by several helper T cell subsets, NK cells, mast cells, eosinophils, basophils, and visceral smooth muscle cells. IL-13 suppresses the production of proinflammatory cytokines and other cytotoxic substances by macrophages, fibroblasts, and endothelial cells. It promotes B cell activation, immunoglobulin class switching to IgE, and the upregulation of CD23/Fc epsilon RII. IL-13 signals through a receptor complex containing IL-13 R alpha 1 and IL-4 R alpha. This complex also functions as the type 2 IL-4 receptor. IL-13 R alpha 2 binds IL-13 and prevents IL-13 from signaling through the IL-13 R alpha 1/IL-4 R alpha complex. IL-13-bound IL-13 R alpha 2 can directly promote tumor cell invasiveness and the development of tissue fibrosis.

Long Name:
Interleukin 13
Entrez Gene IDs:
3596 (Human); 16163 (Mouse); 116553 (Rat); 574325 (Primate)
Alternate Names:
ALRHMGC116789; BHR1interleukin-13; IL13; IL-13; IL-13MGC116788; interleukin 13; MGC116786; NC30; P600

Assay Procedure

GENERAL ELISA PROTOCOL

Plate Preparation

  1. Dilute the Capture Antibody to the working concentration in PBS without carrier protein. Immediately coat a 96-well microplate with 100 μL per well of the diluted Capture Antibody. Seal the plate and incubate overnight at room temperature.
  2. Aspirate each well and wash with Wash Buffer, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (400 μL) using a squirt bottle, manifold dispenser, or autowasher. Complete removal of liquid at each step is essential for good performance. After the last wash, remove any remaining Wash Buffer by aspirating or by inverting the plate and blotting it against clean paper towels.
  3. Block plates by adding 300 μL Reagent Diluent to each well. Incubate at room temperature for a minimum of 1 hour.
  4. Repeat the aspiration/wash as in step 2. The plates are now ready for sample addition.

Assay Procedure

  1. Add 100 μL of sample or standards in Reagent Diluent, or an appropriate diluent, per well. Cover with an adhesive strip and incubate 2 hours at room temperature.
  2. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  3. Add 100 μL of the Detection Antibody, diluted in Reagent Diluent, to each well. Cover with a new adhesive strip and incubate 2 hours at room temperature.
  4. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  5. Add 100 μL of the working dilution of Streptavidin-HRP to each well. Cover the plate and incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  6. Repeat the aspiration/wash as in step 2.
  7. Add 100 μL of Substrate Solution to each well. Incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  8. Add 50 μL of Stop Solution to each well. Gently tap the plate to ensure thorough mixing.
  9. Determine the optical density of each well immediately, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. If wavelength correction is not available, subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.

Citations for Mouse IL-13 DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

87 Citations: Showing 1 - 10
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  1. Repeated Allergen Exposure in A/J Mice Causes Steroid-Insensitive Asthma via a Defect in Glucocorticoid Receptor Bioavailability
    Authors: MF Serra, AC Cotias, CRR Pão, JB Daleprane, PB Jurgilas, GC Couto, EA Anjos-Valo, RSB Cordeiro, VF Carvalho, PMR Silva, MA Martins
    J. Immunol., 2018;0(0):.
    Species: Mouse
    Sample Types: Tissue Lysates
  2. Increases in helminth-induced IL-21 protein levels disappear upon sample freezing
    Authors: A Ariyaratne, EK Szabo, J Bowron, CAM Finney
    Cytokine, 2018;108(0):179-181.
    Species: Mouse
    Sample Types: Tissue Homogenates
  3. Analogous corticosteroids, 9A and EK100, derived from solid-state-cultured mycelium of Antrodia camphorata inhibit proinflammatory cytokine expression in macrophages
    Authors: ST Kao, YH Kuo, SD Wang, HJ Hong, LJ Lin
    Cytokine, 2018;108(0):136-144.
    Species: Mouse
    Sample Types: BALF
  4. IL-33/ST2 axis mediates hyperplasia of intrarenal urothelium in obstructive renal injury
    Authors: WY Chen, JL Yang, YH Wu, LC Li, RF Li, YT Chang, LH Dai, WC Wang, YJ Chang
    Exp. Mol. Med., 2018;50(4):36.
    Species: Mouse
    Sample Types: Serum
  5. TPL-2 restricts Ccl24-dependent immunity to Heligmosomoides polygyrus
    Authors: Y Kannan, LJ Entwistle, VS Pelly, J Perez-Llor, AW Walker, SC Ley, MS Wilson
    PLoS Pathog., 2017;13(7):e1006536.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  6. IGF1R deficiency attenuates acute inflammatory response in a bleomycin-induced lung injury mouse model
    Authors: S Piñeiro-He, IP López, E Alfaro-Arn, R Torrens, M Iñiguez, L Alvarez-Er, C Ruíz-Martí, JG Pichel
    Sci Rep, 2017;7(1):4290.
    Species: Mouse
    Sample Types: Tissue Homogenates
  7. Characterization of the acute inflammatory profile and resolution of airway inflammation after Igf1r-gene targeting in a murine model of HDM-induced asthma
    Authors: S Piñeiro-He, E Alfaro-Arn, JA Gregory, R Torrens, C Ruíz-Martí, M Adner, IP López, JG Pichel
    PLoS ONE, 2017;12(12):e0190159.
    Species: Mouse
    Sample Types: Tissue Homogenates
  8. Experimental atopic dermatitis depends on IL-33R signaling via MyD88 in dendritic cells
    Authors: C Li, I Maillet, C Mackowiak, C Viala, F Di Padova, M Li, D Togbe, V Quesniaux, Y Lai, B Ryffel
    Cell Death Dis, 2017;8(4):e2735.
    Species: Mouse
    Sample Types: Tissue Homogenates
  9. Schistosoma japonicum infection downregulates house dust mite-induced allergic airway inflammation in mice
    Authors: S Qiu, X Fan, Y Yang, P Dong, W Zhou, Y Xu, Y Zhou, F Guo, Y Zheng, JQ Yang
    PLoS ONE, 2017;12(6):e0179565.
    Species: Mouse
    Sample Types: BALF
  10. IRF5 distinguishes severe asthma in humans and drives Th1 phenotype and airway hyperreactivity in mice
    Authors: TB Oriss, M Raundhal, C Morse, RE Huff, S Das, R Hannum, MC Gauthier, KL Scholl, K Chakrabort, SM Nouraie, SE Wenzel, P Ray, A Ray
    JCI Insight, 2017;2(10):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  11. Menin Controls the Memory Th2 Cell Function by Maintaining the Epigenetic Integrity of Th2 Cells
    Authors: A Onodera, M Kiuchi, K Kokubo, M Kato, T Ogino, S Horiuchi, U Kanai, K Hirahara, T Nakayama
    J. Immunol., 2017;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  12. Somatic extracts of Marshallagia marshalli downregulate the Th2 associated immune responses in ovalbumin-induced airway inflammation in BALB/c mice
    Authors: S Parande Sh, A Ebrahimby, A Dousty, M Maleki, A Movassaghi, H Borji, A Haghparast
    Parasit Vectors, 2017;10(1):233.
    Species: Mouse
    Sample Types: BALF
  13. Water-soluble chitosan inhibits nerve growth factor and attenuates allergic inflammation in mite allergen-induced allergic rhinitis
    Authors: PC Chen, MH Hsieh, P Wen-ShuoKu, HF Kao, CL Hsu, JY Wang
    J. Allergy Clin. Immunol., 2017;0(0):.
    Species: Mouse
    Sample Types: NLF
  14. Protective immune responses against Schistosoma mansoni infection by immunization with functionally active gut-derived cysteine peptidases alone and in combination with glyceraldehyde 3-phosphate dehydrogenase
    Authors: H Tallima, J Dvo?ák, S Kareem, M Abou El Da, N Abdel Aziz, JP Dalton, R El Ridi
    PLoS Negl Trop Dis, 2017;11(3):e0005443.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  15. Host DNA released by NETosis promotes rhinovirus-induced type-2 allergic asthma exacerbation
    Authors: M Toussaint, DJ Jackson, D Swieboda, A Guedán, TD Tsouroukts, YM Ching, C Radermecke, H Makrinioti, J Aniscenko, MR Edwards, R Solari, F Farnir, V Papayannop, F Bureau, T Marichal, SL Johnston
    Nat. Med., 2017;23(6):681-691.
    Species: Mouse
    Sample Types: Tissue Homogenates
  16. Mitochondrial CaMKII inhibition in airway epithelium protects against allergic asthma
    Authors: SC Sebag, OM Koval, JD Paschke, CJ Winters, OA Jaffer, R Dworski, FS Sutterwala, ME Anderson, IM Grumbach
    JCI Insight, 2017;2(3):e88297.
    Species: Mouse
    Sample Types: Tissue Homogenates
  17. Attenuated airway hyperresponsiveness and mucus secretion in HDM exposed Igf1r-deficient mice
    Authors: S Piñeiro-He, JA Gregory, IP López, R Torrens, C Ruíz-Martí, M Adner, JG Pichel
    Allergy, 2017;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  18. Acid-Sensing Ion Channel 1a Contributes to Airway Hyperreactivity in Mice
    PLoS ONE, 2016;11(11):e0166089.
    Species: Mouse
    Sample Types: BALF
  19. Advax, a Delta Inulin Microparticle, Potentiates In-built Adjuvant Property of Co-administered Vaccines
    EBioMedicine, 2016;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  20. Editor's Highlight: Subvisible Aggregates of Immunogenic Proteins Promote a Th1-Type Response
    Authors: Jeremy P Derrick
    Toxicol. Sci., 2016;153(2):258-70.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  21. Repeated Activation of Lung Invariant NKT Cells Results in Chronic Obstructive Pulmonary Disease-Like Symptoms.
    Authors: Tsao C, Tsao P, Chen Y, Chuang Y
    PLoS ONE, 2016;11(1):e0147710.
    Species: Mouse
    Sample Types: BALF
  22. Impact of Histone H1 on the Progression of Allergic Rhinitis and Its Suppression by Neutralizing Antibody in Mice
    Authors: T Nakano, R Kamei, T Fujimura, Y Takaoka, A Hori, CY Lai, KC Chiang, Y Shimada, N Ohmori, T Goto, K Ono, CL Chen, S Goto, S Kawamoto
    PLoS ONE, 2016;11(4):e0153630.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  23. Brazilian red propolis improves cutaneous wound healing suppressing inflammation-associated transcription factor NF?B
    Authors: Flavia Regina Sobreira Corrêa
    Biomed. Pharmacother, 2016;86(0):162-171.
    Species: Mouse
    Sample Types: Plasma
  24. DUOX1 mediates persistent epithelial EGFR activation, mucous cell metaplasia, and airway remodeling during allergic asthma
    JCI Insight, 2016;1(18):e88811.
    Species: Mouse
    Sample Types: BALF
  25. Notch regulates Th17 differentiation and controls trafficking of IL-17 and metabolic regulators within Th17 cells in a context-dependent manner
    Sci Rep, 2016;6(0):39117.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  26. Exposure to Aedes aegypti Bites Induces a Mixed-Type Allergic Response following Salivary Antigens Challenge in Mice
    PLoS ONE, 2016;11(5):e0155454.
    Species: Mouse
    Sample Types: BALF
  27. IL-22 Restrains Tapeworm-Mediated Protection against Experimental Colitis via Regulation of IL-25 Expression
    Authors: JL Reyes, MR Fernando, F Lopes, G Leung, NL Mancini, CE Matisz, A Wang, DM McKay
    PLoS Pathog, 2016;12(4):e1005481.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  28. IL-27 Is Essential for Suppression of Experimental Allergic Asthma by the TLR7/8 Agonist R848 (Resiquimod)
    Authors: Gesine Hansen
    J. Immunol., 2016;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  29. Adenovirus-mediated Foxp3 expression in lung epithelial cells reduces airway inflammation in ovalbumin and cockroach-induced asthma model
    Exp Mol Med, 2016;48(9):e259.
    Species: Mouse
    Sample Types: Serum
  30. Perinatal Activation of the Interleukin-33 Pathway Promotes Type 2 Immunity in the Developing Lung
    Authors: Ismé M de Kleer
    Immunity, 2016;45(6):1285-1298.
    Species: Mouse
    Sample Types: Tissue Homogenates
  31. Paired Ig-like Receptor B Inhibits IL-13-Driven Eosinophil Accumulation and Activation in the Esophagus
    Authors: Netali Ben Baruch
    J Immunol, 2016;0(0):.
    Species: Mouse
    Sample Types: BALF
  32. Allergen-Induced IL-6 Regulates IL-9/IL-17A Balance in CD4+ T Cells in Allergic Airway Inflammation
    J Immunol, 2016;197(7):2653-64.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  33. IRAK-M promotes alternative macrophage activation and fibroproliferation in bleomycin-induced lung injury.
    Authors: Ballinger M, Newstead M, Zeng X, Bhan U, Mo X, Kunkel S, Moore B, Flavell R, Christman J, Standiford T
    J Immunol, 2015;194(4):1894-904.
  34. Hydroxypropyl-beta-cyclodextrin spikes local inflammation that induces Th2 cell and T follicular helper cell responses to the coadministered antigen.
    Authors: Onishi M, Ozasa K, Kobiyama K, Ohata K, Kitano M, Taniguchi K, Homma T, Kobayashi M, Sato A, Katakai Y, Yasutomi Y, Wijaya E, Igarashi Y, Nakatsu N, Ise W, Inoue T, Yamada H, Vandenbon A, Standley D, Kurosaki T, Coban C, Aoshi T, Kuroda E, Ishii K
    J Immunol, 2015;194(6):2673-82.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  35. CD252 regulates mast cell mediated, CD1d-restricted NKT-cell activation in mice.
    Authors: Gonzalez Roldan N, Orinska Z, Ewers H, Bulfone-Paus S
    Eur J Immunol, 2015;46(2):432-9.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  36. Differential Immunometabolic Phenotype in Th1 and Th2 Dominant Mouse Strains in Response to High-Fat Feeding.
    Authors: Jovicic N, Jeftic I, Jovanovic I, Radosavljevic G, Arsenijevic N, Lukic M, Pejnovic N
    PLoS ONE, 2015;10(7):e0134089.
    Species: Mouse
    Sample Types: Tissue Homogenates
  37. Superior Suppressive Capacity of Skin Tregs Compared with Lung Tregs in a Model of Epicutaneous Priming.
    Authors: Mahapatra S, Albrecht M, Baru A, Sparwasser T, Herrick C, Dittrich A
    J Invest Dermatol, 2015;135(10):2418-26.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  38. Mice deficient in the St3gal3 gene product alpha2,3 sialyltransferase (ST3Gal-III) exhibit enhanced allergic eosinophilic airway inflammation.
    Authors: Kiwamoto T, Brummet M, Wu F, Motari M, Smith D, Schnaar R, Zhu Z, Bochner B
    J Allergy Clin Immunol, 2014;133(1):240-7.e1-3.
    Species: Mouse
    Sample Types: BALF
  39. IL-25 and type 2 innate lymphoid cells induce pulmonary fibrosis.
    Authors: Hams, Emily, Armstrong, Michelle, Barlow, Jillian, Saunders, Sean P, Schwartz, Christia, Cooke, Gordon, Fahy, Ruairi J, Crotty, Thomas B, Hirani, Nikhil, Flynn, Robin J, Voehringer, David, McKenzie, Andrew N, Donnelly, Seamas C, Fallon, Padraic
    Proc Natl Acad Sci U S A, 2014;111(1):367-72.
    Species: Mouse
    Sample Types: Tissue Homogenates
  40. Delineating the role of histamine-1- and -4-receptors in a mouse model of Th2-dependent antigen-specific skin inflammation.
    Authors: Mahapatra S, Albrecht M, Behrens B, Jirmo A, Behrens G, Hartwig C, Neumann D, Raap U, Bahre H, Herrick C, Dittrich A
    PLoS ONE, 2014;9(2):e87296.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  41. Cyclooxygenase inhibition abrogates aeroallergen-induced immune tolerance by suppressing prostaglandin I2 receptor signaling.
    Authors: Zhou W, Goleniewska K, Zhang J, Dulek D, Toki S, Lotz M, Newcomb D, Boswell M, Polosukhin V, Milne G, Wu P, Moore M, FitzGerald G, Peebles R
    J Allergy Clin Immunol, 2014;134(3):698-705.e5.
    Species: Mouse
    Sample Types: BALF
  42. Foxp3(+) regulatory T cells delay expulsion of intestinal nematodes by suppression of IL-9-driven mast cell activation in BALB/c but not in C57BL/6 mice.
    Authors: Blankenhaus B, Reitz M, Brenz Y, Eschbach M, Hartmann W, Haben I, Sparwasser T, Huehn J, Kuhl A, Feyerabend T, Rodewald H, Breloer M
    PLoS Pathog, 2014;10(2):e1003913.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  43. Retinoic acid prevents mesenteric lymph node dendritic cells from inducing IL-13-producing inflammatory Th2 cells.
    Authors: Yokota-Nakatsuma A, Takeuchi H, Ohoka Y, Kato C, Song S, Hoshino T, Yagita H, Ohteki T, Iwata M
    Mucosal Immunol, 2014;7(4):786-801.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  44. The activating protein 1 transcription factor basic leucine zipper transcription factor, ATF-like (BATF), regulates lymphocyte- and mast cell-driven immune responses in the setting of allergic asthma.
    Authors: Ubel C, Sopel N, Graser A, Hildner K, Reinhardt C, Zimmermann T, Rieker R, Maier A, Neurath M, Murphy K, Finotto S
    J Allergy Clin Immunol, 2014;133(1):198-206.e1-9.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  45. Unique macrophages different from M1/M2 macrophages inhibit T cell mitogenesis while upregulating Th17 polarization.
    Authors: Tatano Y, Shimizu T, Tomioka H
    Sci Rep, 2014;4(0):4146.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  46. Lifetime-dependent effects of bisphenol A on asthma development in an experimental mouse model.
    Authors: Petzold, Susanne, Averbeck, Marco, Simon, Jan C, Lehmann, Irina, Polte, Tobias
    PLoS ONE, 2014;9(6):e100468.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  47. Epithelial NF-kappaB orchestrates house dust mite-induced airway inflammation, hyperresponsiveness, and fibrotic remodeling.
    Authors: Tully J, Hoffman S, Lahue K, Nolin J, Anathy V, Lundblad L, Daphtary N, Aliyeva M, Black K, Dixon A, Poynter M, Irvin C, Janssen-Heininger Y
    J Immunol, 2013;191(12):5811-21.
    Species: Mouse
    Sample Types: Tissue Homogenates
  48. T1/ST2 promotes T helper 2 cell activation and polyfunctionality in bronchopulmonary mycosis.
    Authors: Piehler D, Grahnert A, Eschke M, Richter T, Kohler G, Stenzel W, Alber G
    Mucosal Immunol, 2013;6(2):405-14.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  49. Nanogel-based PspA intranasal vaccine prevents invasive disease and nasal colonization by Streptococcus pneumoniae.
    Authors: Kong, Il Gyu, Sato, Ayuko, Yuki, Yoshikaz, Nochi, Tomonori, Takahashi, Haruko, Sawada, Shinichi, Mejima, Mio, Kurokawa, Shiho, Okada, Kazunari, Sato, Shintaro, Briles, David E, Kunisawa, Jun, Inoue, Yusuke, Yamamoto, Masafumi, Akiyoshi, Kazunari, Kiyono, Hiroshi
    Infect Immun, 2013;81(5):1625-34.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  50. Endogenous retinoids in the pathogenesis of alopecia areata.
    Authors: Duncan F, Silva K, Johnson C, King B, Szatkiewicz J, Kamdar S, Ong D, Napoli J, Wang J, King L, Whiting D, McElwee K, Sundberg J, Everts H
    J Invest Dermatol, 2013;133(2):334-43.
    Species: Mouse
    Sample Types: Tissue Homogenates
  51. Interleukin-33-dependent innate lymphoid cells mediate hepatic fibrosis.
    Authors: McHedlidze T, Waldner M, Zopf S, Walker J, Rankin A, Schuchmann M, Voehringer D, McKenzie A, Neurath M, Pflanz S, Wirtz S
    Immunity, 2013;39(2):357-71.
    Species: Mouse
    Sample Types: Serum
  52. Control of allergen-induced inflammation and hyperresponsiveness by the metalloproteinase ADAMTS-12.
    Authors: Paulissen G, El Hour M, Rocks N, Gueders M, Bureau F, Foidart J, Lopez-Otin C, Noel A, Cataldo D
    J Immunol, 2012;189(8):4135-43.
    Species: Mouse
    Sample Types: Tissue Homogenates
  53. Interleukin-1alpha controls allergic sensitization to inhaled house dust mite via the epithelial release of GM-CSF and IL-33.
    J. Exp. Med., 2012;209(8):1505-17.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  54. Epithelial cell-derived IL-25, but not Th17 cell-derived IL-17 or IL-17F, Is crucial for murine asthma.
    Authors: Suzukawa M, Morita H, Nambu A, Arae K, Shimura E, Shibui A, Yamaguchi S, Suzukawa K, Nakanishi W, Oboki K, Kajiwara N, Ohno T, Ishii A, Korner H, Cua D, Suto H, Yoshimoto T, Iwakura Y, Yamasoba T, Ohta K, Sudo K, Saito H, Okumura K, Broide D, Matsumoto K, Nakae S
    J Immunol, 2012;189(7):3641-52.
    Species: Mouse
    Sample Types: BALF
  55. Immune Modulation Mediated by Cryptococcal Laccase Promotes Pulmonary Growth and Brain Dissemination of Virulent Cryptococcus neoformans in Mice.
    Authors: Qiu Y, Davis M, Dayrit J, Hadd Z, Meister D, Osterholzer J, Williamson P, Olszewski M
    PLoS ONE, 2012;7(10):e47853.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  56. Alveolar Macrophages Play a Key Role in Cockroach-Induced Allergic Inflammation via TNF-alpha Pathway.
    Authors: Kim J, Sohn J, Choi J, Lee J, Hong C, Lee J, Park J
    PLoS ONE, 2012;7(10):e47971.
    Species: Mouse
    Sample Types: Tissue Homogenates
  57. T cell-derived Act1 is necessary for IL-25-mediated Th2 responses and allergic airway inflammation.
    Authors: Swaidani S, Bulek K, Kang Z
    J. Immunol., 2011;187(6):3155-64.
    Species: Mouse
    Sample Types: BALF
  58. Impaired basophil induction leads to an age-dependent innate defect in type 2 immunity during helminth infection in mice.
    Authors: Nel HJ, Hams E, Saunders SP
    J. Immunol., 2011;186(8):4631-9.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  59. High expression of IL-22 suppresses antigen-induced immune responses and eosinophilic airway inflammation via an IL-10-associated mechanism.
    Authors: Nakagome K, Imamura M, Kawahata K
    J. Immunol., 2011;187(10):5077-89.
    Species: Mouse
    Sample Types: BALF
  60. The Therapeutic Potential of the Humoral Pattern Recognition Molecule PTX3 in Chronic Lung Infection Caused by Pseudomonas aeruginosa.
    Authors: Moalli F, Paroni M, Veliz Rodriguez T, Riva F, Polentarutti N, Bottazzi B, Valentino S, Mantero S, Nebuloni M, Mantovani A, Bragonzi A, Garlanda C
    J. Immunol., 2011;186(9):5425-34.
    Species: Mouse
    Sample Types: Tissue Homogenates
  61. IL-33-responsive lineage- CD25+ CD44(hi) lymphoid cells mediate innate type 2 immunity and allergic inflammation in the lungs.
    Authors: Bartemes KR, Iijima K, Kobayashi T, Kephart GM, McKenzie AN, Kita H
    J. Immunol., 2011;188(3):1503-13.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  62. Mucosal delivery of human papillomavirus pseudovirus-encapsidated plasmids improves the potency of DNA vaccination.
    Authors: Graham BS, Kines RC, Corbett KS, Nicewonger J, Roberts JN, Schiller JT, Buck CB
    Mucosal Immunol, 2010;3(5):475-86.
    Species: Mouse
    Sample Types: BALF
  63. Alpha-Galactosylceramide-induced airway eosinophilia is mediated through the activation of NKT cells.
    Authors: Chuang YH, Wang TC, Jen HY, Yu AL, Chiang BL
    J. Immunol., 2010;186(8):4687-92.
    Species: Mouse
    Sample Types: BALF
  64. Th17 cells are the dominant T cell subtype primed by Shigella flexneri mediating protective immunity.
    Authors: Sellge G, Magalhaes JG, Konradt C, Fritz JH, Salgado-Pabon W, Eberl G, Bandeira A, Di Santo JP, Sansonetti PJ, Phalipon A
    J. Immunol., 2010;184(4):2076-85.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  65. A critical role for C5L2 in the pathogenesis of experimental allergic asthma.
    Authors: Zhang X, Schmudde I, Laumonnier Y, Pandey MK, Clark JR, Konig P, Gerard NP, Gerard C, Wills-Karp M, Kohl J
    J. Immunol., 2010;185(11):6741-52.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  66. Elevated asymmetric dimethylarginine alters lung function and induces collagen deposition in mice.
    Authors: Wells SM, Buford MC, Migliaccio CT, Holian A
    Am. J. Respir. Cell Mol. Biol., 2009;40(2):179-88.
    Species: Mouse
    Sample Types: BALF
  67. Resolution of allergic inflammation and airway hyperreactivity is dependent upon disruption of the T1/ST2-IL-33 pathway.
    Authors: Kearley J, Buckland KF, Mathie SA, Lloyd CM
    Am. J. Respir. Crit. Care Med., 2009;179(9):772-81.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  68. Effects of umbelliferone in a murine model of allergic airway inflammation.
    Authors: Vasconcelos JF, Teixeira MM, Barbosa-Filho JM, Agra MF, Nunes XP, Giulietti AM, Ribeiro-Dos-Santos R, Soares MB
    Eur. J. Pharmacol., 2009;609(1):126-31.
    Species: Mouse
    Sample Types: BALF
  69. Identification of cardiac troponin I sequence motifs leading to heart failure by induction of myocardial inflammation and fibrosis.
    Authors: Kaya Z, Goser S, Buss SJ, Leuschner F, Ottl R, Li J, Volkers M, Zittrich S, Pfitzer G, Rose NR, Katus HA
    Circulation, 2008;118(20):2063-72.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  70. Identification of functional roles for both IL-17RB and IL-17RA in mediating IL-25-induced activities.
    Authors: Rickel EA, Siegel LA, Yoon BR, Rottman JB, Kugler DG, Swart DA, Anders PM, Tocker JE, Comeau MR, Budelsky AL
    J. Immunol., 2008;181(6):4299-310.
    Species: Mouse
    Sample Types: BALF
  71. Surfactant protein D alters allergic lung responses in mice and human subjects.
    Authors: Brandt EB, Mingler MK, Stevenson MD, Wang N, Khurana Hershey GK, Whitsett JA, Rothenberg ME
    J. Allergy Clin. Immunol., 2008;121(5):1140-1147.e2.
    Species: Mouse
    Sample Types: BALF
  72. Transforming growth factor-beta regulates house dust mite-induced allergic airway inflammation but not airway remodeling.
    Authors: Fattouh R, Midence NG, Arias K, Johnson JR, Walker TD, Goncharova S, Souza KP, Gregory RC, Lonning S, Gauldie J, Jordana M
    Am. J. Respir. Crit. Care Med., 2008;177(6):593-603.
    Species: Mouse
    Sample Types: BALF
  73. Induction of IL-33 expression and activity in central nervous system glia.
    Authors: Hudson CA, Christophi GP, Gruber RC, Wilmore JR, Lawrence DA, Massa PT
    J. Leukoc. Biol., 2008;84(3):631-43.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  74. Distinct roles for IL-13 and IL-4 via IL-13 receptor alpha1 and the type II IL-4 receptor in asthma pathogenesis.
    Authors: Munitz A, Brandt EB, Mingler M, Finkelman FD, Rothenberg ME
    Proc. Natl. Acad. Sci. U.S.A., 2008;105(20):7240-5.
    Species: Mouse
    Sample Types: BALF
  75. An alternative pathway of NF-kappaB activation results in maturation and T cell priming activity of dendritic cells overexpressing a mutated IkappaBalpha.
    Authors: Moore F, Buonocore S, Aksoy E, Ouled-Haddou N, Goriely S, Lazarova E, Paulart F, Heirman C, Vaeremans E, Thielemans K, Goldman M, Flamand V
    J. Immunol., 2007;178(3):1301-11.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  76. TIM-1 and TIM-3 enhancement of Th2 cytokine production by mast cells.
    Authors: Nakae S, Iikura M, Suto H, Akiba H, Umetsu DT, DeKruyff RH, Saito H, Galli SJ
    Blood, 2007;110(7):2565-8.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  77. Dendritic cell expression of OX40 ligand acts as a costimulatory, not polarizing, signal for optimal Th2 priming and memory induction in vivo.
    Authors: Jenkins SJ, Perona-Wright G, Worsley AG, Ishii N, MacDonald AS
    J. Immunol., 2007;179(6):3515-23.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  78. IL-21 is produced by NKT cells and modulates NKT cell activation and cytokine production.
    Authors: Coquet JM, Kyparissoudis K, Pellicci DG, Besra G, Berzins SP, Smyth MJ, Godfrey DI
    J. Immunol., 2007;178(5):2827-34.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  79. Therapeutic targeting of CC ligand 21 or CC chemokine receptor 7 abrogates pulmonary fibrosis induced by the adoptive transfer of human pulmonary fibroblasts to immunodeficient mice.
    Authors: Pierce EM, Carpenter K, Jakubzick C, Kunkel SL, Flaherty KR, Martinez FJ, Hogaboam CM
    Am. J. Pathol., 2007;170(4):1152-64.
    Species: Mouse
    Sample Types: Tissue Homogenates
  80. Pulmonary distribution, regulation, and functional role of Trk receptors in a murine model of asthma.
    Authors: Nassenstein C, Dawbarn D, Pollock K, Allen SJ, Erpenbeck VJ, Spies E, Krug N, Braun A
    J. Allergy Clin. Immunol., 2006;118(3):597-605.
    Species: Mouse
    Sample Types: BALF
  81. A central regulatory role for eosinophils and the eotaxin/CCR3 axis in chronic experimental allergic airway inflammation.
    Authors: Fulkerson PC, Fischetti CA, McBride ML, Hassman LM, Hogan SP, Rothenberg ME
    Proc. Natl. Acad. Sci. U.S.A., 2006;103(44):16418-23.
    Species: Mouse
    Sample Types: Tissue Homogenates
  82. Interleukin-17 is a negative regulator of established allergic asthma.
    Authors: Schnyder-Candrian S, Togbe D, Couillin I, Mercier I, Brombacher F, Quesniaux V, Fossiez F, Ryffel B, Schnyder B
    J. Exp. Med., 2006;203(12):2715-25.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  83. Inhibition of angiogenesis by interleukin-4 gene therapy in rat adjuvant-induced arthritis.
    Authors: Haas CS, Amin MA, Allen BB, Ruth JH, Haines GK, Woods JM, Koch AE
    Arthritis Rheum., 2006;54(8):2402-14.
    Species: Rat
    Sample Types: Tissue Homogenates
  84. Induced trefoil factor family 1 expression by trans-differentiating Clara cells in a murine asthma model.
    Authors: Kouznetsova I, Chwieralski CE, Balder R, Hinz M, Braun A, Krug N, Hoffmann W
    Am. J. Respir. Cell Mol. Biol., 2006;36(3):286-95.
    Species: Mouse
    Sample Types: BALF
  85. Induction of prolonged infiltration of T lymphocytes and transient T lymphocyte-dependent collagen deposition in mouse lungs following adenoviral gene transfer of CCL18.
    Authors: Luzina IG, Papadimitriou JC, Anderson R, Pochetuhen K, Atamas SP
    Arthritis Rheum., 2006;54(8):2643-55.
    Species: Mouse
    Sample Types: BALF
  86. Hierarchical suppression of asthma-like responses by mucosal tolerance.
    Authors: Keller AC, Mucida D, Gomes E, Faquim-Mauro E, Faria AM, Rodriguez D, Russo M
    J. Allergy Clin. Immunol., 2006;117(2):283-90.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  87. Leukemia inhibitory factor is linked to regulatory transplantation tolerance.
    Authors: Metcalfe SM, Watson TJ, Shurey S, Adams E, Green CJ
    Transplantation, 2005;79(6):726-30.
    Species: Mouse
    Sample Types: Cell Culture Supernates

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Mouse IL-13 DuoSet ELISA
By Anonymous on 01/12/2018
Sample Tested: mouse bronchial alveolar fluid