Mouse IL-15R alpha APC-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB5511A-100
Mouse IL-15R alpha  APC-conjugated Antibody in Data
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Mouse IL-15R alpha APC-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse IL-15 R alpha in direct ELISAs.
Source
Monoclonal Rat IgG2B Clone # 888220
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse IL-15 R alpha
Gly33-Lys205
Accession # Q60819
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of IL-15 Ra antibody in C57BL/6 Mouse Bone Marrow Cells antibody by Flow Cytometry. View Larger

Detection of IL‑15 R alpha in C57BL/6 Mouse Bone Marrow Cells by Flow Cytometry. C57BL/6 mouse bone marrow cells treated with 20 ng/mL Recombinant Mouse GM-CSF (Catalog # 415-ML) for 5 days, 1 µg/mL LPS on day 6, and harvested on day 7 were stained with Rat Anti-Mouse IL-15 Ra APC-conjugated Monoclonal Antibody (Catalog # FAB5511A, filled histogram) or isotype control antibody (Catalog # IC013A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IL-15R alpha

Interleukin 15 Receptor alpha (IL-15 R alpha ) is a high affinity receptor that specifically binds IL-15 with high affinity and associates as a heterotrimer with the IL-2 receptors beta and gamma subunits to initiate signal transduction. IL-15 R alpha is expressed on a wide variety of T cells and B cells as well as non-lymphoid cells. IL‑15 R alpha is a 58-60 kDa protein that shares structural similarities to the IL-2 R alpha protein. IL-15 R alpha and IL-2 R alpha genes also share similar intron-exon organization and are closely linked on human chromosome 10p14-p15. Human IL-15 R alpha shares 45% amino acid (aa) homology with the mouse form of the receptor. Signaling of IL-15 can occur in one of three ways; through the heterotrimeric complex of IL-15 R alpha, IL-2 R beta and IL-2 R gamma c, through the heterodimeric complex of IL-2 receptors beta and gamma common, and through a novel 60-65 kDa IL-15 RX subunit found on mast cells. The binding of IL-15 to IL-15 R alpha has been reported to antagonize the TNF‑ alpha ‑mediated apoptosis in fibroblasts by competing with TNFRI for TRAF2 binding.

Long Name
Interleukin 15 Receptor alpha
Entrez Gene IDs
3601 (Human); 16169 (Mouse); 102121571 (Cynomolgus Monkey)
Alternate Names
CD215 antigen; CD215; IL15 R alpha; IL-15 R alpha; IL-15 receptor subunit alpha; IL-15R alpha; IL15RA; IL-15Ra; IL-15R-alpha; interleukin 15 receptor, alpha; interleukin-15 receptor subunit alpha; MGC104179

Product Datasheets

Citation for Mouse IL-15R alpha APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Reprogramming the lung microenvironment by inhaled immunotherapy fosters immune destruction of tumor
    Authors: Valentino Le Noci
    Oncoimmunology, 2016;5(11):e1234571.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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