Mouse IL-17/IL-17A Alexa Fluor® 647-conjugated Antibody

Catalog # Availability Size / Price Qty
IC7211R-025
Detection of IL‑17/IL‑17A in Mouse Splenocytes Stimulated to Induce Th17 Cells by Flow Cytometry.
2 Images
Product Details
FAQs
Supplemental Products
Reviews

Mouse IL-17/IL-17A Alexa Fluor® 647-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse IL‑17/IL‑17A in direct ELISAs.
Source
Monoclonal Rat IgG2B Clone # 881309
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant mouse IL‑17/IL‑17A
Thr22-Ala158
Accession # Q62386
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 647

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
5 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Intracellular Staining by Flow Cytometry Detection of IL‑17/IL‑17A in Mouse Splenocytes Stimulated to Induce Th17 Cells by Flow Cytometry. View Larger

Detection of IL‑17/IL‑17A in Mouse Splenocytes Stimulated to Induce Th17 Cells by Flow Cytometry. Mouse splenocytes stimulated to induce Th17 cells were stained with Rat Anti-Mouse CD4 PE‑conjugated Monoclonal Antibody (Catalog # FAB554P) and either (A) Rat Anti-Mouse IL‑17/IL‑17A Alexa Fluor® 647‑conjugated Monoclonal Antibody (Catalog # IC7211R) or (B) Rat IgG2B Alexa Fluor 647 Isotype Control (Catalog # IC013R). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Intracellular Staining by Flow Cytometry Detection of IL‑17/IL‑17A in Mouse Splenocytes Stimulated to Induce Th17 Cells by Flow Cytometry. View Larger

Detection of IL‑17/IL‑17A in Mouse Splenocytes Stimulated to Induce Th17 Cells by Flow Cytometry. Mouse splenocytes stimulated to induce Th17 cells were stained with Anti-Mouse IL‑17/IL‑17A Phycoerythrin‑conjugated Monoclonal Antibody (Competitor) and Rat Anti-Mouse CD4 APC‑conjugated Monoclonal Antibody (Catalog # FAB554A). Quadrant markers were set based on control antibody staining (Catalog # IC013P). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IL-17/IL-17A

Interleukin 17 (IL-17), also known as IL-17A and CTLA-8, is a T cell-expressed pleiotropic cytokine that exhibits a high degree of homology to a protein encoded by the ORF13 gene of herpes virus Saimiri. cDNA clones encoding IL-17 have been isolated from activated rat, mouse and human T cells. Mouse IL-17 cDNA encodes a 158 amino acid (aa) residue precursor protein with a 26 amino acid residue signal peptide that is cleaved to yield the 132 aa residue mature  IL-17. Both recombinant and natural IL-17 have been shown to exist as disulfide linked homodimers and IL-17 is typically found as a heterodimer with IL-17F. At the amino acid level, mouse IL-17 shows 57%, 61%, and 87% sequence identity with herpes virus, human, and rat IL-17, respectively. An IL-17 specific mouse cell surface receptor (IL-17 R) has been cloned. While the expression of IL-17 mRNA is restricted to activated alpha beta TCR+CD4-CD8- T cells, the expression of mouse IL-17 R mRNA has been detected in virtually all cells and tissues tested. IL-17 has multiple biological effects on a variety of cells including the induction of IL-6 and IL-8 production by fibroblasts, the enhancement of surface expression of ICAM-1 on fibroblasts, and the activation of NF-kappa B and costimulation of proliferation by T cells.

Long Name
Interleukin 17
Entrez Gene IDs
3605 (Human); 16171 (Mouse); 301289 (Rat); 481837 (Canine)
Alternate Names
CTLA-8; CTLA8cytotoxic T-lymphocyte-associated serine esterase 8; Cytotoxic T-lymphocyte-associated antigen 8; IL17; IL-17; IL17A; IL-17A; IL-17Acytotoxic T-lymphocyte-associated protein 8; IL-17CTLA-8; IL17interleukin-17A; interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8); interleukin 17A

Product Datasheets

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reviews for Mouse IL-17/IL-17A Alexa Fluor® 647-conjugated Antibody

There are currently no reviews for this product. Be the first to review Mouse IL-17/IL-17A Alexa Fluor® 647-conjugated Antibody and earn rewards!

Have you used Mouse IL-17/IL-17A Alexa Fluor® 647-conjugated Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review