Mouse IL-17/IL-17A PE-conjugated Antibody
Mouse IL-17/IL-17A PE-conjugated Antibody Summary
Accession # Q62386
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of IL‑17/IL‑17A in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes stimulated to induce Th17 cells were stained with Rat Anti-Mouse CD4 APC-conjugated Monoclonal Antibody (Catalog # FAB554A) and either (A) Rat Anti-Mouse IL-17/IL-17A PE-conjugated Monoclonal Antibody (Catalog # IC7211P) or (B) Rat IgG2BPhycoerythrin Isotype Control (Catalog # IC013P). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Interleukin 17 (IL-17), also known as IL-17A and CTLA-8, is a T cell-expressed pleiotropic cytokine that exhibits a high degree of homology to a protein encoded by the ORF13 gene of herpes virus Saimiri. cDNA clones encoding IL-17 have been isolated from activated rat, mouse and human T cells. Mouse IL-17 cDNA encodes a 158 amino acid (aa) residue precursor protein with a 26 amino acid residue signal peptide that is cleaved to yield the 132 aa residue mature IL-17. Both recombinant and natural IL-17 have been shown to exist as disulfide linked homodimers and IL-17 is typically found as a heterodimer with IL-17F. At the amino acid level, mouse IL-17 shows 57%, 61%, and 87% sequence identity with herpes virus, human, and rat IL-17, respectively. An IL-17 specific mouse cell surface receptor (IL-17 R) has been cloned. While the expression of IL-17 mRNA is restricted to activated alpha beta TCR+CD4-CD8- T cells, the expression of mouse IL-17 R mRNA has been detected in virtually all cells and tissues tested. IL-17 has multiple biological effects on a variety of cells including the induction of IL-6 and IL-8 production by fibroblasts, the enhancement of surface expression of ICAM-1 on fibroblasts, and the activation of NF-kappa B and costimulation of proliferation by T cells.
No product specific FAQs exist for this product, however you mayView all Antibody FAQs
Reviews for Mouse IL-17/IL-17A PE-conjugated Antibody
There are currently no reviews for this product. Be the first to review Mouse IL-17/IL-17A PE-conjugated Antibody and earn rewards!
Have you used Mouse IL-17/IL-17A PE-conjugated Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image