Mouse IL-17 RB PE-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB10402P-100
FAB10402P-025
Detection of IL‑17 RB in CHO Chinese Hamster Cell Line Transfected with Mouse IL-17 RB by Flow Cytometry.
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Mouse IL-17 RB PE-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse IL-17 RB in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) IL-17 RB, rhIL-17 R,  or recombinant mouse IL-17 is observed.
Source
Monoclonal Rat IgG1 Clone # 752101
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse IL-17 RB
Arg18-Gly286
Accession # Q9JIP3
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of IL‑17 RB in CHO Chinese Hamster Cell Line Transfected with Mouse IL-17 RB by Flow Cytometry. View Larger

Detection of IL‑17 RB in CHO Chinese Hamster Cell Line Transfected with Mouse IL-17 RB by Flow Cytometry. CHO Chinese hamster ovary cell line transfected with mouse IL-17 RB was stained with Rat Anti-Mouse IL‑17 RB PE‑conjugated Monoclonal Antibody (Catalog # FAB10402P, filled histogram) or isotype control antibody (Catalog # IC005P, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IL-17 RB

The Interleukin 17 (IL-17) family of cytokines, comprising six members (IL-17, IL-17B through IL-17F), are structurally related proteins with a conserved cysteine-knot structure. These proinflammatory cytokines can induce local cytokine production and are involved in the regulation of the immune response. The cognate receptors activated by some of these cytokines have been identified (1, 2). Interleukin-17B Receptor (IL-17 RB), also known as IL-17 Rh1, IL-17E R and EVI27, represents the second receptor of the IL-17 family cytokines to be recognized (2‑4). Mouse IL-17 RB cDNA encodes a 499 amino acid (aa) type I membrane protein with a putative 17 aa signal peptide, a 269 aa extracellular domain, a 21 aa transmembrane domain and a 192 aa cytoplasmic tail. As a result of alternative splicing, a secreted variant of IL-17 RB also exists (5). Human and mouse IL‑17 RB share 76% aa sequence identity. IL-17 RB is approximately 20% identical to the human and mouse IL‑17 R. However, the receptors share many conserved cysteine residues within their extracellular domains as well as additional conserved elements within their cytoplasmic domains. At least three additional type I transmembrane receptors with homology to IL‑17 R, including IL-17 RL (IL-17 RC), IL-17 RD, and IL-17 RE, have been reported (2, 6). By Northern blot analysis, mouse IL-17 RB is highly expressed in liver and testes and is expressed at lower levels in kidney and lung. It is also expressed in some hematopoietic cell lines, including selected T cell, B cell, and myeloid cell lines (2‑4). The expression of IL-17 RB is significantly upregulated under inflammatory conditions (7). IL-17 RB binds strongly to IL-17E and weakly to IL-17B. It does not bind IL-17 or IL-17F. Activation of IL-17 RB by its ligands results in the activation of nuclear factor kappa-B (2‑4).

References
  1. Aggarwal, S. and A.L. Gurney (2002) J. Leukoc. Biol. 71:1.
  2. Moseley, T.A. et al. (2003) Cytokine & Growth Factor Rev. 14:155.
  3. Shi, Y. et al. (2000) J. Biol. Chem. 275:19167.
  4. Lee, J. et al. (2001) J. Biol. Chem. 276:1660.
  5. Tian, E. et al. (2000) Oncogene 19:2098.
  6. Haudenschild, D. et al. (2002) J. Biol. Chem. 277:4309.
  7. Hurst, S.D. et al. (2002) J. Immunol. 169:443.
Long Name
Interleukin 17B Receptor
Entrez Gene IDs
55540 (Human); 50905 (Mouse)
Alternate Names
CRL4IL-17 receptor homolog 1; cytokine receptor CRL4; Cytokine receptor-like 4; Evi27; EVI27IL-17B receptor; IL-17 RB; IL-17 receptor B; IL-17 RH1; IL17BRinterleukin 17B receptor; IL-17ER; IL17RB; IL-17RB; IL17Rh1; IL-17Rh1; IL17RH1MGC5245; interleukin 17 receptor B; interleukin 17 receptor homolog 1; interleukin-17 receptor B; Interleukin-17B receptor

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