Mouse IL-17C Antibody
Mouse IL-17C Antibody Summary
Accession # NP_665833
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of IL‑17C in Tramp‑C1 Mouse Cell Line by Flow Cytometry. Tramp‑C1 mouse prostate cancer cell line was stained with Rat Anti-Mouse IL‑17C Monoclonal Antibody (Catalog # MAB23061, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG F(ab')2Secondary Antibody (Catalog # F0105B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
The Interleukin 17 (IL-17) family proteins, comprising six members (IL-17, IL-17B through IL-17F), are secreted, structurally related proteins that share a conserved cysteine-knot fold near the C-terminus, but have considerable sequence divergence at the N-terminus (1, 2). With the exception of IL-17B, which exists as a non‑covalently linked dimer, all IL-17 family members are disulfide-linked dimers (3). IL-17 family proteins are pro-inflammatory cytokines that induce local cytokine production and are involved in the regulation of immune functions (1, 2). Two receptors (IL-17 R, and IL-17B R), which are activated by IL-17 family members have been identified. In addition, at least three additional orphan type I transmembrane receptors with homology to IL-17 R, including IL-17 RL (IL-17 RC), IL-17 RD, and
IL‑17 RE, have also been reported (1‑4). The functions of IL-17 RC, D, and E are not known.
Mouse IL-17C cDNA encodes a 194 amino acid (aa) residues protein with a putative 14 aa signal peptide (5). Although there are no potential N-linked glycosylation sites, it is reportedly glycosylated (6). IL-17C shares from 15%‑30% aa sequence identity with other IL-17 family members. Mouse and human IL-17C share 83% aa sequence identity. IL-17C has a very restricted expression pattern and was detected as a rare expressed sequence tag (EST) in an adult prostate and fetal kidney libraries (2). IL-17C has been shown to stimulate the release of TNF-alpha and IL-1 beta from the monocytic cell line THP-1, a property it shares with IL-17B (6, 7). Human IL‑17C is active on mouse cells (5). The receptor of IL-17C has not yet been identified. The IL-17C preparations from R&D Systems have been found to bind immobilized recombinant mouse IL-17B R/Fc in a functional ELISA.
- Aggarwal, S. and A.L. Gurney (2002) J. Leukoc. Biol. 71:1.
- Moseley, T.A. et al. (2003) Cytokine & Growth Factor Rev. 14:155.
- Hymowitz, S.G. et al. (2001) EMBO J. 20:5332.
- Haudenschild, D. et al. (2002) J. Biol. Chem. 277:4309.
- Hurst, S.D. et al. (2002) J. Immunology 169:443.
- Li, H. et al. (2000) Proc. Natl. Acad. Sci. USA 97:773.
- Shi, Y. et al. (2000) J. Biol. Chem. 275:19167.
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