Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Western Blot, Intracellular Staining by Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 312724
View all IL-17D Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse IL‑17D
Ala25-Arg205
Accession # NP_665836

Specificity

Detects mouse IL-17D in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant human IL-17D, recombinant mouse (rm) IL-17, rmIL-17B, rmIL-17C, rmIL-17E, or rmIL-17F is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Mouse IL‑17D Antibody

Detection of IL‑17D antibody in Mouse Splenocytes antibody by Flow Cytometry.

Detection of IL‑17D in Mouse Splenocytes by Flow Cytometry.

Mouse splenocytes were stained with Rat Anti-Mouse IL-17D Monoclonal Antibody (Catalog # MAB2274, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG F(ab')2Secondary Antibody (Catalog # F0113). To facilitate intracellular staining, cells were fixed with para-formaldehyde and permeabilized with saponin.
Detection of IL-17D by Flow Cytometry

Detection of IL-17D by Flow Cytometry

Xylanase improved the pathogen resistance of Nile tilapia by regulating IL-17D-Reg3 gamma signaling pathway. A Experiment design. Nile tilapia was fed with soybean meal-based diet with or without 3000 U/kg xylanase for 8 weeks (n = 3 tanks, 30 fish per tank). B Survival rate of Nile tilapia against A. hydrophila infection of SM and SMC group (n = 3 tanks, 10 fish per tank). C The significantly upregulated and downregulated genes related to immunity between SM and SMC group (n = 4). D The differently KEGG pathway between SM and SMC group (n = 4). E The gene expression in il-17 signaling pathway (n = 6). F The gene expression of il-17d in the intestine and the concentration of IL-17D in serum (n = 6). G The gene expression of reg3g in the intestine and the concentration of Reg3 gamma in serum (n = 6). il-17d was silenced in fish fed with SMC diet. H Survival rate of Nile tilapia against A. hydrophila infection of SM, SMC, and SMC + il-17d silenced fish (n = 3 tanks, 10 fish per tank). I The gene expression of il-17d in the intestine (n = 6). J the concentration of IL-17D in serum. K The gene expression of reg3g in the intestine (n = 6). Data was expressed as mean ± SEM. SM, fish fed with soybean meal-based diet; SMC, fish fed with soybean meal-based diet supplemented with 3000 U/kg xylanase; SMC + dsRNA, il-17d silenced fish fed with soybean meal-based diet supplemented with 3000 U/kg xylanase. The significant differences between two group were presented at P < 0.05 (*) Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/39434145), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of IL-17D by Flow Cytometry

Detection of IL-17D by Flow Cytometry

Butyrate increased the IL-17D production in intestinal epithelial cells in vivo and in vitro. A, B, C Flow cytometry gating shows IL-17D analysis in viable intestine cells, lymphocytes cells, and epithelial cells from SM and SB fish, D the gene expression of il-17d in intestinal epithelial cells from SM and SB fish, the effects of sodium butyrate on the expression of Eil-17d, and F reg3g in primary intestinal epithelial cells. Data was expressed as mean ± SEM (n = 3). SM, fish fed with soybean meal-based diet; SB, fish fed with soybean meal-based diet supplemented with 40 mmol/kg sodium butyrate. The significant differences between two group were presented at P < 0.05 (*) based on Student’s test. Statistically significant results were expressed by lowercase letters (a, b, c) based on ANOVA with Tukey test Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/39434145), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of IL-17D by Flow Cytometry

Detection of IL-17D by Flow Cytometry

Butyrate increased the IL-17D production in intestinal epithelial cells in vivo and in vitro. A, B, C Flow cytometry gating shows IL-17D analysis in viable intestine cells, lymphocytes cells, and epithelial cells from SM and SB fish, D the gene expression of il-17d in intestinal epithelial cells from SM and SB fish, the effects of sodium butyrate on the expression of Eil-17d, and F reg3g in primary intestinal epithelial cells. Data was expressed as mean ± SEM (n = 3). SM, fish fed with soybean meal-based diet; SB, fish fed with soybean meal-based diet supplemented with 40 mmol/kg sodium butyrate. The significant differences between two group were presented at P < 0.05 (*) based on Student’s test. Statistically significant results were expressed by lowercase letters (a, b, c) based on ANOVA with Tukey test Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/39434145), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Mouse IL‑17D Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Intracellular Staining by Flow Cytometry

2.5 µg/106 cells
Sample: Mouse splenocytes fixed with paraformaldehyde and permeabilized with saponin

Western Blot

1 µg/mL
Sample: Recombinant Mouse IL‑17D (Catalog # 2274-ML)

Reviewed Applications

Read 2 reviews rated 4.5 using MAB2274 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-17D

The Interleukin 17 (IL-17) family proteins, comprising six members (IL-17, IL-17B through IL-17F), are secreted, structurally related proteins that share a conserved cysteine-knot fold near the C-terminus, but have considerable sequence divergence at the N‑terminus (1, 2, 6). With the exception of IL-17B, which exists as a non‑covalently linked dimer, all IL-17 family members are disulfide-linked dimers (3). IL-17 family proteins are pro-inflammatory cytokines that induce local cytokine production and are involved in the regulation of immune functions (1, 2, 6). Two receptors (IL-17 R, and IL-17B R), which are activated by IL-17 family members, have been identified. In addition, at least three additional orphan type I transmembrane receptors with homology to IL-17 R, including IL-17 RL (IL-17 RC), IL-17 RD, and IL-17 RE, have also been reported (1‑6). Mouse IL-17D is synthesized as a 205 amino acid (aa) precursor that contains a putative 24 aa signal peptide and a 181 aa mature segment. The mature region contains two potential N-linked glycosylation sites and eight cysteines, four of which are involved in the formation of a modified cysteine-knot motif (5). The molecule is reported to exist as a 53 kDa disulfide-linked homodimer (2, 5). Given that its predicted homodimeric molecular weight is 40 kDa, the molecule is presumably glycosylated. In the mature region, mouse IL-17D is 88% aa identical to human IL-17D. There is less than 30% aa identity between mouse IL-17D and other members of the mouse IL-17 family. IL-17D is expressed in skeletal muscle, adipose tissue, fetal liver, and heart, plus resting CD4+ T cells and CD19+ B cells (1). R&D Systems has shown IL-17D binding to a mouse IL-17 R/Fc construct in a functional ELISA. IL-17D is known to induce the production of IL-8, IL-6 and GM-CSF (5).

References

  1. Aggarwal, S. and A.L. Gurney (2002) J. Leukoc. Biol. 71:1.
  2. Moseley, T.A. et al. (2003) Cytokine & Growth Factor Rev. 14:155.
  3. Hymowitz, S.G. et al. (2001) EMBO J. 20:5332.
  4. Haudenschild, D. et al. (2002) J. Biol. Chem. 277:4309.
  5. Starnes, T. et al. (2002) J. Immunol. 169:642.
  6. Kolls, J.K. and A. Linden (2004) Immunity 21:467.

Long Name

Interleukin 17D

Alternate Names

IL17D

Entrez Gene IDs

53342 (Human); 239114 (Mouse)

Gene Symbol

IL17D

UniProt

NP_665836

Additional IL-17D Products

Product Documents for Mouse IL‑17D Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse IL‑17D Antibody

For research use only

Related Research Areas

Helper T (Th) Cells

Citations for Mouse IL‑17D Antibody

Customer Reviews for Mouse IL‑17D Antibody (2)

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  • Mouse IL-17D Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Recombinant protein
    Species: Mouse
    Verified Customer | Posted 05/15/2022
    Mouse IL‑17D Antibody MAB2274
  • Mouse IL-17D Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Recombinant protein
    Species: Mouse
    Verified Customer | Posted 05/21/2016
    Mouse IL‑17D Antibody MAB2274

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Associated Pathways

IL-17 Family Signaling Pathways IL-17 Family Signaling Pathway Thumbnail