Mouse IL-17D Antibody Summary
Ala25-Arg205
Accession # NP_665836
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of IL‑17D in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse IL-17D Monoclonal Antibody (Catalog # MAB2274, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG F(ab')2Secondary Antibody (Catalog # F0113). To facilitate intracellular staining, cells were fixed with para-formaldehyde and permeabilized with saponin.
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Detection of IL-17D by Flow Cytometry Xylanase improved the pathogen resistance of Nile tilapia by regulating IL-17D-Reg3 gamma signaling pathway. A Experiment design. Nile tilapia was fed with soybean meal-based diet with or without 3000 U/kg xylanase for 8 weeks (n = 3 tanks, 30 fish per tank). B Survival rate of Nile tilapia against A. hydrophila infection of SM and SMC group (n = 3 tanks, 10 fish per tank). C The significantly upregulated and downregulated genes related to immunity between SM and SMC group (n = 4). D The differently KEGG pathway between SM and SMC group (n = 4). E The gene expression in il-17 signaling pathway (n = 6). F The gene expression of il-17d in the intestine and the concentration of IL-17D in serum (n = 6). G The gene expression of reg3g in the intestine and the concentration of Reg3 gamma in serum (n = 6). il-17d was silenced in fish fed with SMC diet. H Survival rate of Nile tilapia against A. hydrophila infection of SM, SMC, and SMC + il-17d silenced fish (n = 3 tanks, 10 fish per tank). I The gene expression of il-17d in the intestine (n = 6). J the concentration of IL-17D in serum. K The gene expression of reg3g in the intestine (n = 6). Data was expressed as mean ± SEM. SM, fish fed with soybean meal-based diet; SMC, fish fed with soybean meal-based diet supplemented with 3000 U/kg xylanase; SMC + dsRNA, il-17d silenced fish fed with soybean meal-based diet supplemented with 3000 U/kg xylanase. The significant differences between two group were presented at P < 0.05 (*) Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/39434145), licensed under a CC-BY license. Not internally tested by R&D Systems.
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Detection of IL-17D by Flow Cytometry Butyrate increased the IL-17D production in intestinal epithelial cells in vivo and in vitro. A, B, C Flow cytometry gating shows IL-17D analysis in viable intestine cells, lymphocytes cells, and epithelial cells from SM and SB fish, D the gene expression of il-17d in intestinal epithelial cells from SM and SB fish, the effects of sodium butyrate on the expression of Eil-17d, and F reg3g in primary intestinal epithelial cells. Data was expressed as mean ± SEM (n = 3). SM, fish fed with soybean meal-based diet; SB, fish fed with soybean meal-based diet supplemented with 40 mmol/kg sodium butyrate. The significant differences between two group were presented at P < 0.05 (*) based on Student’s test. Statistically significant results were expressed by lowercase letters (a, b, c) based on ANOVA with Tukey test Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/39434145), licensed under a CC-BY license. Not internally tested by R&D Systems.
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Detection of IL-17D by Flow Cytometry Butyrate increased the IL-17D production in intestinal epithelial cells in vivo and in vitro. A, B, C Flow cytometry gating shows IL-17D analysis in viable intestine cells, lymphocytes cells, and epithelial cells from SM and SB fish, D the gene expression of il-17d in intestinal epithelial cells from SM and SB fish, the effects of sodium butyrate on the expression of Eil-17d, and F reg3g in primary intestinal epithelial cells. Data was expressed as mean ± SEM (n = 3). SM, fish fed with soybean meal-based diet; SB, fish fed with soybean meal-based diet supplemented with 40 mmol/kg sodium butyrate. The significant differences between two group were presented at P < 0.05 (*) based on Student’s test. Statistically significant results were expressed by lowercase letters (a, b, c) based on ANOVA with Tukey test Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/39434145), licensed under a CC-BY license. Not internally tested by R&D Systems.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-17D
The Interleukin 17 (IL-17) family proteins, comprising six members (IL-17, IL-17B through IL-17F), are secreted, structurally related proteins that share a conserved cysteine-knot fold near the C-terminus, but have considerable sequence divergence at the N‑terminus (1, 2, 6). With the exception of IL-17B, which exists as a non‑covalently linked dimer, all IL-17 family members are disulfide-linked dimers (3). IL-17 family proteins are pro-inflammatory cytokines that induce local cytokine production and are involved in the regulation of immune functions (1, 2, 6). Two receptors (IL-17 R, and IL-17B R), which are activated by IL-17 family members, have been identified. In addition, at least three additional orphan type I transmembrane receptors with homology to IL-17 R, including IL-17 RL (IL-17 RC), IL-17 RD, and IL-17 RE, have also been reported (1‑6). Mouse IL-17D is synthesized as a 205 amino acid (aa) precursor that contains a putative 24 aa signal peptide and a 181 aa mature segment. The mature region contains two potential N-linked glycosylation sites and eight cysteines, four of which are involved in the formation of a modified cysteine-knot motif (5). The molecule is reported to exist as a 53 kDa disulfide-linked homodimer (2, 5). Given that its predicted homodimeric molecular weight is 40 kDa, the molecule is presumably glycosylated. In the mature region, mouse IL-17D is 88% aa identical to human IL-17D. There is less than 30% aa identity between mouse IL-17D and other members of the mouse IL-17 family. IL-17D is expressed in skeletal muscle, adipose tissue, fetal liver, and heart, plus resting CD4+ T cells and CD19+ B cells (1). R&D Systems has shown IL-17D binding to a mouse IL-17 R/Fc construct in a functional ELISA. IL-17D is known to induce the production of IL-8, IL-6 and GM-CSF (5).
- Aggarwal, S. and A.L. Gurney (2002) J. Leukoc. Biol. 71:1.
- Moseley, T.A. et al. (2003) Cytokine & Growth Factor Rev. 14:155.
- Hymowitz, S.G. et al. (2001) EMBO J. 20:5332.
- Haudenschild, D. et al. (2002) J. Biol. Chem. 277:4309.
- Starnes, T. et al. (2002) J. Immunol. 169:642.
- Kolls, J.K. and A. Linden (2004) Immunity 21:467.
Product Datasheets
Citation for Mouse IL-17D Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Interleukin-17D and Nrf2 mediate initial innate immune cell recruitment and restrict MCMV infection
Authors: R Seelige, R Saddawi-Ko, NM Adams, G Picarda, JC Sun, CA Benedict, JD Bui
Sci Rep, 2018-09-12;8(1):13670.
Species: Mouse
Sample Types: Peritoneal Lavage Fluid
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