Detection of IL‑23 in RAW 264.7 Mouse Cell Line by Flow Cytometry.
RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse IL‑23 APC‑conjugated Monoclonal Antibody (Catalog # IC18871A, filled histogram) or isotype control antibody (Catalog # IC006A, open histogram). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Interleukin-23 is a heterodimeric, secreted cytokine that
belongs to the IL-12 family of molecules.
Mouse IL-23 is composed of two disulfide-linked chains, p40, a 40-42 kDa,
313 amino acid (aa) glycosylated protein that also contributes to the formation
of IL-12, and p19, a 19-21 kDa, 175 aa polypeptide that also contributes to the
formation of IL-39. It has been found to be principally expressed by activated
macrophages and dendritic cells, where it acts on naïve CD4+ T cells and
promotes the development of T-helper 17 type cells. It is also reported to
drive the formation of osteoblasts from mesenchymal stem cells. The receptor complex for IL-23 is composed of
two chains, IL-23R and IL-12Rb1. Over
the amino acid ranges cited for this product, mouse p19 shares 74% and 88% aa
sequence identity with human and rat p19 respectively, while mouse p40 shares
66% and 93% aa sequence identity with human and rat p40, respectively.
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