Mouse IL-23 APC-conjugated Antibody

IC18871A has been discontinued. View all IL-23 products.
  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse IL-23 in flow cytometry.
  • Source
    Monoclonal Rat IgG2A Clone # 320244
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant mouse IL-23 p19 and recombinant mouse IL-12/IL-23 p40
    Leu20-Ala196 (Il-23 p19) & Met23-Ser335 (IL-12/IL-23 p40)
    Accession # Q9EQ14 (Il-23 p19) & P43432 (IL-12/IL-23 p40)
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Allophycocyanin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Intracellular Staining by Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Intracellular Staining by Flow Cytometry
Detection of IL‑23 in RAW 264.7 Mouse Cell Line by Flow Cytometry. RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse IL‑23 APC‑conjugated Monoclonal Antibody (Catalog # IC18871A, filled histogram) or isotype control antibody (Catalog # IC006A, open histogram). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: IL-23
Interleukin-23 is a heterodimeric, secreted cytokine that belongs to the IL-12 family of molecules. Mouse IL-23 is composed of two disulfide-linked chains, p40, a 40-42 kDa, 313 amino acid (aa) glycosylated protein that also contributes to the formation of IL-12, and p19, a 19-21 kDa, 175 aa polypeptide that also contributes to the formation of IL-39. It has been found to be principally expressed by activated macrophages and dendritic cells, where it acts on naïve CD4+ T cells and promotes the development of T-helper 17 type cells. It is also reported to drive the formation of osteoblasts from mesenchymal stem cells. The receptor complex for IL-23 is composed of two chains, IL-23R and IL-12Rb1. Over the amino acid ranges cited for this product, mouse p19 shares 74% and 88% aa sequence identity with human and rat p19 respectively, while mouse p40 shares 66% and 93% aa sequence identity with human and rat p40, respectively.
  • Long Name:
    Interleukin 23
  • Entrez Gene IDs:
    51561 (Human); 83430 (Mouse)
  • Alternate Names:
    IL23; IL-23; IL23A; IL-23A; IL-23-A; IL-23p19; IL23P19P19; interleukin 23 p19 subunit; interleukin 23, alpha subunit p19; interleukin-23 subunit alpha; Interleukin-23 subunit p19; JKA3 induced upon T-cell activation; MGC79388; SGRF; SGRFIL-23 subunit alpha
Related Research Areas

Have you used Mouse IL-23 APC-conjugated Antibody?

Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Order Details

Contact Information

Calculators

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷
R&D Systems Guarantee

Monoclonal
Antibody
Panels For
Screening

Learn More
Monoclonal Antibodies

Feedback

Customer Information