Mouse IL-23 PE-conjugated Antibody

Catalog # Availability Size / Price Qty
IC18871P
Detection of IL‑23 in RAW 264.7 Mouse Cell Line by Flow Cytometry.
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Mouse IL-23 PE-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse IL-23 in flow cytometry.
Source
Monoclonal Rat IgG2A Clone # 320244
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant mouse IL-23 p19 and recombinant mouse IL-12/IL-23 p40
Leu20-Ala196 (Il-23 p19) & Met23-Ser335 (IL-12/IL-23 p40)
Accession # Q9EQ14 (Il-23 p19) & P43432 (IL-12/IL-23 p40)
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry Detection of IL‑23 antibody in RAW 264.7 Mouse Cell Line antibody by Flow Cytometry. View Larger

Detection of IL‑23 in RAW 264.7 Mouse Cell Line by Flow Cytometry. RAW 264.7 mouse monocyte/macrophage cell line untreated (open histogram) or treated with LPS (filled histogram) was stained with Rat Anti-Mouse IL-23 PE-conjugated Monoclonal Antibody (Catalog # IC18871P, filled histogram) or isotype control antibody (Catalog # IC006P, data not shown). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: IL-23

Interleukin 23 (IL-23) is a heterodimeric cytokine composed of two disulfide-linked subunits, a p19 subunit that is unique to IL-23, and a p40 subunit that is shared with IL-12 (1-5). The p19 subunit has homology to the p35 subunit of IL-12, as well as to other single chain cytokines such as IL-6 and IL-11. The p40 subunit is homologous to the extracellular domains of the hematopoietic cytokine receptors. Mouse p19 cDNA encodes a 196 amino acid residue (aa) precursor protein with a putative 19 aa signal peptide and 177 aa mature protein. Human and mouse p19 share 70% aa sequence identity. Although p19 is expressed by activated macrophages, dendritic cells, T cells, and endothelial cells, only activated macrophages and dendritic cells express p40 concurrently to produce IL-23. The functional IL-23 receptor complex consists of two receptor subunits, the IL-12 receptor beta 1 subunit (IL-12 R beta 1) and the IL-23-specific receptor subunit (IL-23 R). IL-23 has biological activities that are similar to, but distinct from IL-12. Both IL-12 and IL-23 induce proliferation and IFN-gamma production by human T cells. While IL-12 acts on both naïve and memory human T cells, the effects of IL-23 is restricted to memory T cells. In mouse, IL-23 but not IL-12, has also been shown to induce memory T cells to secret IL-17, a potent pro-inflammatory cytokine. IL-12 and IL-23 can induce IL-12 production from mouse splenic DC of both the CD8- and CD8+ subtypes, however only IL-23 can act directly on CD8+ DC to mediate immunogenic presentation of poorly immunogenic tumor/self peptide.

References
  1. Oppmann, B. et al. (2000) Immunity 13:715.
  2. Lankford, C.S. and D.M. Frucht (2003) J. Leukoc. Biol. 73:49.
  3. Parham, C. et al. (2002) J. Immunol. 168:5699.
  4. Belladonna, M.L. et al. (2002) J. Immunol. 168:5448.
  5. Aggarwal, S. et al. (2003) J. Biol. Chem. 278:1910.
Long Name
Interleukin 23
Entrez Gene IDs
51561 (Human); 83430 (Mouse); 155140 (Rat); 102128555 (Cynomolgus Monkey)
Alternate Names
IL-23 p19/IL-12 p40; IL23; IL-23; IL-23A; IL-23-A; IL-23p19; IL-23p19/IL-12p40; IL23P19P19; interleukin 23 p19 subunit; interleukin 23, alpha subunit p19; interleukin-23 subunit alpha; Interleukin-23 subunit p19; JKA3 induced upon T-cell activation; MGC79388; SGRF; SGRFIL-23 subunit alpha

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Citation for Mouse IL-23 PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Exacerbated Imiquimod-Induced Psoriasis-Like Skin Inflammation in IRF5-Deficient Mice
    Authors: M Nakao, T Miyagaki, M Sugaya, S Sato
    Int J Mol Sci, 2020-05-23;21(10):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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