Mouse IL-23 Quantikine ELISA Kit

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  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (50 ug), Serum (50 ug), Heparin Plasma (50 ug)
  • Sensitivity
    4.17 pg/mL
  • Assay Range
    15.6 - 1,000 pg/mL (Cell Culture Supernates, Serum, Heparin Plasma)
  • Specificity
    Natural and recombinant mouse IL-23. This assay does not recognize free p19 or p40 subunits.
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    Interference observed with 1 or more available related molecules.
Product Summary
The Quantikine Mouse IL-23 immunoassay is a 4.5 hour solid-phase ELISA designed to measure IL-23 in cell culture supernates, mouse serum, and plasma. It contains Sf 21-expressed recombinant mouse IL-23 and antibodies raised against the recombinant p19 and p40 subunits. This immunoassay has been shown to accurately quantitate the recombinant mouse IL-23. Results obtained using natural mouse IL-23 showed dose response curves that were parallel to the standard curves obtained using the Quantikine mouse kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse IL-23.

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum, Heparin Plasma
Intra-Assay Precision Inter-Assay Precision
Standard Deviation3.725.8422.73.697.1418.6


The recovery of mouse IL-23 spiked to three levels throughout the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Supernates (n=4) 103 99-108
Heparin Plasma (n=4) 99 88-117
Serum (n=4) 95 90-101
To assess the linearity of the assay, samples spiked with high concentrations of mouse IL-23 in each matrix were diluted with Calibrator Diluent and assayed.
Mouse IL-23 Quantikine ELISA Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: IL-23
IL-23 (Interleukin-23) is a disulfide-linked cytokine composed of a p19 subunit that is unique to IL-23 and a p40 subunit that is shared with IL-12. It is produced by activated macrophages, microglia, and monocyte-derived dendritic cells in response to pathogens. IL-23 induces the earliest recruitment of neutrophils to the site of infection and promotes the development and maintenance of Th17 cells. It also enhances the development of Th17 mediated autoimmunity and tumor progression. IL-23 signals through a receptor complex consisting of IL-12 R beta 1 and IL-23 R. This complex is expressed in mouse Th1 and Th2 cells, bone marrow dendritic cells, activated macrophages and CD4+ CD45Rb(low) memory T cells.
    • Long Name
      Interleukin 23
    • Entrez Gene IDs
      51561 (Human); 83430 (Mouse);
    • Alternate Names
      IL23; IL-23; IL23A; IL-23A; IL-23-A; IL-23p19; IL23P19P19; interleukin 23 p19 subunit; interleukin 23, alpha subunit p19; interleukin-23 subunit alpha; Interleukin-23 subunit p19; JKA3 induced upon T-cell activation; MGC79388; SGRF; SGRFIL-23 subunit alpha;
    Related Research Areas
    Assay Procedure
    Refer to the product for complete assay procedure.

    Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
    1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
    2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

    3. 50 µL Assay Diluent
    4.   Add 50 µL of Assay Diluent to each well.

    5. 50 µL Standard, Control, or Sample
    6.   Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
    7.   Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.

    8. 100 µL Conjugate
    9.   Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
    10.   Aspirate and wash 5 times.

    11. 100 µL Substrate Solution
    12.   Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.

    13. 100 µL Stop Solution
    14.   Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    Showing Results 1 - 10 of 10
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    Sample Type
    1. Endogenous n-3 polyunsaturated fatty acids protect against imiquimod-induced psoriasis-like inflammation via the IL-17/IL-23 axis.
      Authors: Qin S, Wen J, Bai X, Chen T, Zheng R, Zhou G, Ma J, Feng J, Zhong B, Li Y
      Mol Med Rep, 2014;9(6):2097-104.
      Species: Mouse
      Sample Type: Serum
    2. Steady-state neutrophil homeostasis is dependent on TLR4/TRIF signaling.
      Authors: Bugl S, Wirths S, Radsak M, Schild H, Stein P, Andre M, Muller M, Malenke E, Wiesner T, Marklin M, Frick J, Handgretinger R, Rammensee H, Kanz L, Kopp H
      Blood, 2013;121(5):723-33.
      Species: Mouse
      Sample Type: Plasma
    3. A protective role for human IL-10-expressing CD4+ T cells in colitis.
      J. Immunol., 2012;189(3):1243-52.
      Species: Mouse
      Sample Type: Tissue Homogenates
    4. Elevating body temperature enhances hematopoiesis and neutrophil recovery after total body irradiation in an IL-1-, IL-17-, and G-CSF-dependent manner.
      Blood, 2012;120(13):2600-9.
      Species: Mouse
      Sample Type: tissue lysates
    5. Human mesenchymal stem/stromal cells cultured as spheroids are self-activated to produce prostaglandin E2 that directs stimulated macrophages into an anti-inflammatory phenotype.
      Authors: Ylostalo J, Bartosh T, Coble K, Prockop D
      Stem Cells, 2012;30(10):2283-96.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    6. Influenza A inhibits Th17-mediated host defense against bacterial pneumonia in mice.
      Authors: Kudva A, Scheller EV, Robinson KM
      J. Immunol., 2011;186(3):1666-74.
      Species: Mouse
      Sample Type: BALF
    7. Co-adjuvant effects of retinoic acid and IL-15 induce inflammatory immunity to dietary antigens.
      Authors: DePaolo RW, Abadie V, Tang F
      Nature, 2011;471(7337):220-4.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    8. Morphine Inhibits Murine Dendritic Cell IL-23 Production by Modulating Toll-like Receptor 2 and Nod2 Signaling.
      Authors: Wang J, Ma J, Charboneau R
      J. Biol. Chem., 2011;286(12):10225-32.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    9. Morphine disrupts interleukin-23 (IL-23)/IL-17-mediated pulmonary mucosal host defense against Streptococcus pneumoniae infection.
      Authors: Ma J, Wang J, Wan J, Charboneau R, Chang Y, Barke RA, Roy S
      Infect. Immun., 2010;78(2):830-7.
      Species: Mouse
      Sample Type: BALF
    10. CXCL12 (SDF-1alpha) suppresses ongoing experimental autoimmune encephalomyelitis by selecting antigen-specific regulatory T cells.
      Authors: Meiron M, Zohar Y, Anunu R, Wildbaum G, Karin N
      J. Exp. Med., 2008;205(11):2643-55.
      Species: Mouse
      Sample Type: Cell Culture Supernates
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