Mouse IL-23 Quantikine ELISA Kit

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M2300
Mouse IL-23 ELISA Standard Curve
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Product Details
Procedure
Citations (20)
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Mouse IL-23 Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (50 uL), Heparin Plasma (50 uL)
Sensitivity
4.17 pg/mL
Assay Range
15.6 - 1,000 pg/mL (Cell Culture Supernates, Serum, Heparin Plasma)
Specificity
Natural and recombinant mouse IL-23. This assay does not recognize free p19 or p40 subunits.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
Interference observed with 1 or more available related molecules.

Product Summary

The Quantikine Mouse IL-23 immunoassay is a 4.5 hour solid-phase ELISA designed to measure IL-23 in cell culture supernates, mouse serum, and plasma. It contains Sf 21-expressed recombinant mouse IL-23 and antibodies raised against the recombinant p19 and p40 subunits. This immunoassay has been shown to accurately quantitate the recombinant mouse IL-23. Results obtained using natural mouse IL-23 showed dose response curves that were parallel to the standard curves obtained using the Quantikine mouse kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse IL-23.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean 49.3 107 344 50.4 116 333
Standard Deviation 3.72 5.84 22.7 3.69 7.14 18.6
CV% 7.5 5.5 6.6 7.3 6.2 5.6

Recovery

The recovery of mouse IL-23 spiked to three levels throughout the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Supernates (n=4) 103 99-108
Heparin Plasma (n=4) 99 88-117
Serum (n=4) 95 90-101

Linearity

To assess the linearity of the assay, samples spiked with high concentrations of mouse IL-23 in each matrix were diluted with Calibrator Diluent and assayed.
Mouse IL-23 ELISA Linearity

Data Examples

Mouse IL-23 ELISA Standard Curve

Product Datasheets

Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-23

IL-23 (Interleukin-23) is a disulfide-linked cytokine composed of a p19 subunit that is unique to IL-23 and a p40 subunit that is shared with IL-12. It is produced by activated macrophages, microglia, and monocyte-derived dendritic cells in response to pathogens. IL-23 induces the earliest recruitment of neutrophils to the site of infection and promotes the development and maintenance of Th17 cells. It also enhances the development of Th17 mediated autoimmunity and tumor progression. IL-23 signals through a receptor complex consisting of IL-12 R beta 1 and IL-23 R. This complex is expressed in mouse Th1 and Th2 cells, bone marrow dendritic cells, activated macrophages and CD4+ CD45Rb(low) memory T cells.

Long Name:
Interleukin 23
Entrez Gene IDs:
51561 (Human); 83430 (Mouse); 155140 (Rat)
Alternate Names:
IL-23 p19/IL-12 p40; IL23; IL-23; IL-23A; IL-23-A; IL-23p19; IL-23p19/IL-12p40; IL23P19P19; interleukin 23 p19 subunit; interleukin 23, alpha subunit p19; interleukin-23 subunit alpha; Interleukin-23 subunit p19; JKA3 induced upon T-cell activation; MGC79388; SGRF; SGRFIL-23 subunit alpha
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 50 µL Assay Diluent
  4.   Add 50 µL of Assay Diluent to each well.

  5. 50 µL Standard, Control, or Sample
  6.   Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
  7.   Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.

  8. 100 µL Conjugate
  9.   Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
  10.   Aspirate and wash 5 times.

  11. 100 µL Substrate Solution
  12.   Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.

  13. 100 µL Stop Solution
  14.   Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Mouse IL-23 Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

20 Citations: Showing 1 - 10
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  1. Exacerbated Imiquimod-Induced Psoriasis-Like Skin Inflammation in IRF5-Deficient Mice
    Authors: M Nakao, T Miyagaki, M Sugaya, S Sato
    Int J Mol Sci, 2020;21(10):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  2. Fenoldopam mesylate for treating psoriasis: A new indication for an old drug
    Authors: S Doppalapud, A Jain, W Khan, AJ Domb
    Int J Pharm, 2019;0(0):118726.
    Species: Mouse
    Sample Types: Tissue Homogenates
  3. Lactobacillus delbrueckii subsp. bulgaricus 2038 and Streptococcus thermophilus 1131 Induce the Expression of the REG3 Family in the Small Intestine of Mice via the Stimulation of Dendritic Cells and Type 3 Innate Lymphoid Cells
    Authors: K Kobayashi, Y Honme, T Sashihara
    Nutrients, 2019;11(12):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  4. Mesenchymal Stem Cells Alleviate Moderate-to-Severe Psoriasis by Reducing the Production of Type I Interferon (IFN-I) by Plasmacytoid Dendritic Cells (pDCs)
    Authors: M Chen, J Peng, Q Xie, N Xiao, X Su, H Mei, Y Lu, J Zhou, Y Dai, S Wang, C Li, G Lin, L Cheng
    Stem Cells Int, 2019;2019(0):6961052.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  5. Preventative effects of the partial RANKL peptide MHP1-AcN in a mouse model of imiquimod-induced psoriasis
    Authors: N Ju, M Shimamura, H Hayashi, Y Ikeda, S Yoshida, A Nakamura, R Morishita, H Rakugi, H Nakagami
    Sci Rep, 2019;9(1):15434.
    Species: Mouse
    Sample Types: Serum
  6. Modulation of Gut Microbiota Composition by Serotonin Signaling Influences Intestinal Immune Response and Susceptibility to Colitis
    Authors: YH Kwon, H Wang, E Denou, JE Ghia, L Rossi, ME Fontes, SP Bernier, MS Shajib, S Banskota, SM Collins, MG Surette, WI Khan
    Cell Mol Gastroenterol Hepatol, 2019;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  7. Tumoral NOX4 recruits M2 tumor-associated macrophages via ROS/PI3K signaling-dependent various cytokine production to promote NSCLC growth
    Authors: J Zhang, H Li, Q Wu, Y Chen, Y Deng, Z Yang, L Zhang, B Liu
    Redox Biol, 2019;22(0):101116.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  8. RIG-I antiviral signaling drives interleukin-23 production and psoriasis-like skin disease
    Authors: H Zhu, F Lou, Q Yin, Y Gao, Y Sun, J Bai, Z Xu, Z Liu, W Cai, F Ke, L Zhang, H Zhou, H Wang, G Wang, X Chen, H Zhang, Z Wang, F Ginhoux, C Lu, B Su, H Wang
    EMBO Mol Med, 2017;9(5):589-604.
    Species: Mouse
    Sample Types: Tissue Homogenates
  9. Depletion of Foxp3+ regulatory T cells increases severity of mechanical allodynia and significantly alters systemic cytokine levels following peripheral nerve injury.
    Authors: Lees J, Duffy S, Perera C, Moalem-Taylor G
    Cytokine, 2015;71(2):207-14.
    Species: Mouse
    Sample Types: Serum
  10. Endogenous n-3 polyunsaturated fatty acids protect against imiquimod-induced psoriasis-like inflammation via the IL-17/IL-23 axis.
    Authors: Qin S, Wen J, Bai X, Chen T, Zheng R, Zhou G, Ma J, Feng J, Zhong B, Li Y
    Mol Med Rep, 2014;9(6):2097-104.
    Species: Mouse
    Sample Types: Serum
  11. Steady-state neutrophil homeostasis is dependent on TLR4/TRIF signaling.
    Authors: Bugl S, Wirths S, Radsak M, Schild H, Stein P, Andre M, Muller M, Malenke E, Wiesner T, Marklin M, Frick J, Handgretinger R, Rammensee H, Kanz L, Kopp H
    Blood, 2013;121(5):723-33.
    Species: Mouse
    Sample Types: Plasma
  12. A protective role for human IL-10-expressing CD4+ T cells in colitis.
    J. Immunol., 2012;189(3):1243-52.
    Species: Mouse
    Sample Types: Tissue Homogenates
  13. Human mesenchymal stem/stromal cells cultured as spheroids are self-activated to produce prostaglandin E2 that directs stimulated macrophages into an anti-inflammatory phenotype.
    Authors: Ylostalo J, Bartosh T, Coble K, Prockop D
    Stem Cells, 2012;30(10):2283-96.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  14. Elevating body temperature enhances hematopoiesis and neutrophil recovery after total body irradiation in an IL-1-, IL-17-, and G-CSF-dependent manner.
    Blood, 2012;120(13):2600-9.
    Species: Mouse
    Sample Types: Tissue Homogenates
  15. Influenza A inhibits Th17-mediated host defense against bacterial pneumonia in mice.
    Authors: Kudva A, Scheller EV, Robinson KM
    J. Immunol., 2011;186(3):1666-74.
    Species: Mouse
    Sample Types: BALF
  16. Co-adjuvant effects of retinoic acid and IL-15 induce inflammatory immunity to dietary antigens.
    Authors: DePaolo RW, Abadie V, Tang F
    Nature, 2011;471(7337):220-4.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  17. Morphine Inhibits Murine Dendritic Cell IL-23 Production by Modulating Toll-like Receptor 2 and Nod2 Signaling.
    Authors: Wang J, Ma J, Charboneau R
    J. Biol. Chem., 2011;286(12):10225-32.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  18. Morphine disrupts interleukin-23 (IL-23)/IL-17-mediated pulmonary mucosal host defense against Streptococcus pneumoniae infection.
    Authors: Ma J, Wang J, Wan J, Charboneau R, Chang Y, Barke RA, Roy S
    Infect. Immun., 2010;78(2):830-7.
    Species: Mouse
    Sample Types: BALF
  19. CXCL12 (SDF-1alpha) suppresses ongoing experimental autoimmune encephalomyelitis by selecting antigen-specific regulatory T cells.
    Authors: Meiron M, Zohar Y, Anunu R, Wildbaum G, Karin N
    J. Exp. Med., 2008;205(11):2643-55.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  20. Central Role of IL-23 and IL-17 Producing Eosinophils as Immunomodulatory Effector Cells in Acute Pulmonary Aspergillosis and Allergic Asthma.
    Authors: Guerra E, Lee C, Specht C, Yadav B, Huang H, Akalin A, Huh J, Mueller C, Levitz S
    PLoS Pathog, 0;13(1):e1006175.
    Species: Mouse
    Sample Types: BALF

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