Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse, Transgenic Mouse

Applications

Validated:

Western Blot, Intracellular Staining by Flow Cytometry, Immunocytochemistry, CyTOF-ready

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Neutralization, Flow Cytometry, Immunocytochemistry, ELISA Development, In vivo assay

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 396118
View all IL-33 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse IL-33
Ser109-Ile266
Accession # Q8BVZ5

Specificity

Detects mouse IL-33 in direct ELISAs.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Mouse IL‑33 Antibody

Detection of Mouse IL-33 antibody by Western Blot.

Detection of Mouse IL‑33 by Western Blot.

Western blot shows lysates of mouse splenocytes. PVDF membrane was probed with 0.5 µg/mL of Rat Anti-Mouse IL-33 Monoclonal Antibody (Catalog # MAB3626) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). Specific bands were detected for IL-33 at approximately 18 and 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
IL‑33 antibody in bEnd by Immunocytochemistry (ICC).3 Mouse Cell Line by Immunocytochemistry (ICC).

IL‑33 in bEnd.3 Mouse Cell Line.

IL-33 was detected in immersion fixed bEnd.3 mouse endothelioma cell line using Rat Anti-Mouse IL-33 Monoclonal Antibody (Catalog # MAB3626) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red, upper panel, Catalog # NL013) and counterstained with DAPI (blue, lower panel). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of IL-33 antibody in bEnd.3 Mouse Cell Line antibody by Flow Cytometry.

Detection of IL‑33 in bEnd.3 Mouse Cell Line by Flow Cytometry.

bEnd.3 mouse endothelioma cell line was stained with Rat Anti-Mouse IL-33 Monoclonal Antibody (Catalog # MAB3626, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Applications for Mouse IL‑33 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed bEnd.3 mouse endothelioma cell line

Intracellular Staining by Flow Cytometry

0.25 µg/106 cells
Sample: bEnd.3 mouse endothelioma cell line fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)

Western Blot

0.5 µg/mL
Sample: Mouse splenocytes

Reviewed Applications

Read 4 reviews rated 4.8 using MAB3626 in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-33

IL-33, also known as NF-HEV and DVS 27, is a 30 kDa proinflammatory protein that may also regulate gene transcription (1-3). DVS 27 was identifed as a gene that is upregulated in vasospastic cerebral arteries (1). NF-HEV was described as a nuclear factor that is preferentially expressed in the endothelial cells of high endothelial venules relative to endothelial cells from other tissues (2). IL-33 was identified based on sequence and structural homology with IL-1 family cytokines (3). DVS 27, NF-HEV, and IL-33 share 100% amino acid sequence identity. IL-33 is constitutively expressed in smooth muscle and airway epithelia. It is upregulated in arterial smooth muscle, dermal fibroblasts, and keratinocytes following IL-1 alpha or IL-1 beta stimulation (1, 3). Similar to IL-1, IL-33 can be cleaved in vitro by caspase-1, generating an N-terminal fragment that is slightly shorter than the C-terminal fragment (3, 4). The N-terminal portion of full length IL-33 contains a predicted bipartite nuclear localization sequence and a homeodomain-like helix-turn-helix DNA binding domain. By immunofluorescence, full length IL-33 localizes to the nucleus in HUVECs and transfectants (2). The C-terminal fragment, corresponding to mature IL-33, binds and triggers signaling through mast cell IL-1 R4/ST2L, a longtime orphan receptor involved in the augmentation of Th2 cell responses (3, 5-7). A ternary signaling complex is formed by the subsequent association of IL-33 and ST2L with IL-1R AcP (8). Stimulation of Th2 polarized lymphocytes with mature IL-33 in vitro induces IL-5 and IL-13 secretion (3). In vivo administration of mature IL-33 promotes increased production of IL-5, IL-13, IgE, and IgA, as well as splenomegaly and inflammatory infiltration of mucosal tissues (3). Full length and mature mouse IL-33 share approximately 55% and 90% amino acid (aa) sequence identity with human and rat IL-33, respectively. Mouse IL-33 shares less than 25% aa sequence identity with other IL-1 family proteins.

References

  1. Onda, H. et al. (1999) J. Cereb. Blood Flow Metab. 19:1279.
  2. Baekkevold, E.S. et al. (2003) Am. J. Pathol. 163:69.
  3. Schmitz, J. et al. (2005) Immunity 23:479.
  4. Black, R.A. et al. (1989) J. Biol. Chem. 264:5323.
  5. Xu, D. et al. (1998) J. Exp. Med. 187:787.
  6. Lohning, M. et al. (1998) Proc. Natl. Acad. Sci. USA 95:6930.
  7. Dinarello, C.A. (2005) Immunity 23:461.
  8. Chackerian, A.A. et al. (2007) J. Immunol. 179:2551.
 

Long Name

Interleukin 33

Alternate Names

C9orf26, DVS27, IL33, NF-HEV

Entrez Gene IDs

90865 (Human); 77125 (Mouse)

Gene Symbol

IL33

UniProt

Q8BVZ5

Additional IL-33 Products

Product Documents for Mouse IL‑33 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse IL‑33 Antibody

For research use only

Citations for Mouse IL‑33 Antibody

Customer Reviews for Mouse IL‑33 Antibody (4)

4.8 out of 5
4 Customer Ratings
5 Stars
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4 Stars
25%
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1 Stars
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Customer Images

Showing  1 - 4 of 4 reviews Showing All
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  • Mouse IL-33 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Retinal Pigment Epithelium cells
    Species: Mouse
    Verified Customer | Posted 02/17/2022
    Mouse IL‑33 Antibody MAB3626
  • Mouse IL-33 Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Lung tissue
    Species: Mouse
    Verified Customer | Posted 09/29/2021
    Mouse IL‑33 Antibody MAB3626
  • Mouse IL-33 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Cell Lysates
    Species: Mouse
    Verified Customer | Posted 07/22/2016
  • Mouse IL‑33 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Sputum
    Verified Customer | Posted 10/26/2015
    Loaded 5mcg protein/lane. Primary incubated O/N at 4 degrees with rocking. <br />Specificity: Reasonably specific<br />Sensitivity: Reasonably sensitive<br />Buffer: TBS-Tween, 5% non-fat milk<br />Dilution: 1:1000
    Mouse IL‑33 Antibody MAB3626

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Protocols

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