Mouse IL-33 Antibody

(14 citations)
(2 Reviews)
  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse IL-33 in direct ELISAs.
  • Source
    Monoclonal Rat IgG2A Clone # 396118
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant mouse IL-33
    Ser109-Ile266
    Accession # Q8BVZ5
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.5 µg/mL
    See below
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Immunocytochemistry
    8-25 µg/mL
    See below
  • Intracellular Staining by Flow Cytometry
    0.25 µg/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Mouse IL‑33 by Western Blot. Western blot shows lysates of mouse splenocytes. PVDF membrane was probed with 0.5 µg/mL of Rat Anti-Mouse IL‑33 Monoclonal Antibody (Catalog # MAB3626) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). Specific bands were detected for IL‑33 at approximately 18 and 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunocytochemistry
IL‑33 in bEnd.3 Mouse Cell Line. IL‑33 was detected in immersion fixed bEnd.3 mouse endothelioma cell line using Rat Anti-Mouse IL‑33 Monoclonal Antibody (Catalog # MAB3626) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red, upper panel, Catalog # NL013) and counterstained with DAPI (blue, lower panel). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Intracellular Staining by Flow Cytometry
Detection of IL‑33 in bEnd.3 Mouse Cell Line by Flow Cytometry. bEnd.3 mouse endothelioma cell line was stained with Rat Anti-Mouse IL‑33 Monoclonal Antibody (Catalog # MAB3626, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-33

IL-33, also known as NF-HEV and DVS 27, is a 30 kDa proinflammatory protein that may also regulate gene transcription (1-3). DVS 27 was identifed as a gene that is upregulated in vasospastic cerebral arteries (1). NF-HEV was described as a nuclear factor that is preferentially expressed in the endothelial cells of high endothelial venules relative to endothelial cells from other tissues (2). IL-33 was identified based on sequence and structural homology with IL-1 family cytokines (3). DVS 27, NF-HEV, and IL-33 share 100% amino acid sequence identity. IL-33 is constitutively expressed in smooth muscle and airway epithelia. It is upregulated in arterial smooth muscle, dermal fibroblasts, and keratinocytes following IL-1 alpha or IL-1 beta stimulation (1, 3). Similar to IL-1, IL-33 can be cleaved in vitro by caspase-1, generating an N-terminal fragment that is slightly shorter than the C-terminal fragment (3, 4). The N-terminal portion of full length IL-33 contains a predicted bipartite nuclear localization sequence and a homeodomain-like helix-turn-helix DNA binding domain. By immunofluorescence, full length IL-33 localizes to the nucleus in HUVECs and transfectants (2). The C-terminal fragment, corresponding to mature IL-33, binds and triggers signaling through mast cell IL-1 R4/ST2L, a longtime orphan receptor involved in the augmentation of Th2 cell responses (3, 5-7). A ternary signaling complex is formed by the subsequent association of IL-33 and ST2L with IL-1R AcP (8). Stimulation of Th2 polarized lymphocytes with mature IL-33 in vitro induces IL-5 and IL-13 secretion (3). In vivo administration of mature IL-33 promotes increased production of IL-5, IL-13, IgE, and IgA, as well as splenomegaly and inflammatory infiltration of mucosal tissues (3). Full length and mature mouse IL-33 share approximately 55% and 90% amino acid (aa) sequence identity with human and rat IL-33, respectively. Mouse IL-33 shares less than 25% aa sequence identity with other IL-1 family proteins.

  • References:
    1. Onda, H. et al. (1999) J. Cereb. Blood Flow Metab. 19:1279.
    2. Baekkevold, E.S. et al. (2003) Am. J. Pathol. 163:69.
    3. Schmitz, J. et al. (2005) Immunity 23:479.
    4. Black, R.A. et al. (1989) J. Biol. Chem. 264:5323.
    5. Xu, D. et al. (1998) J. Exp. Med. 187:787.
    6. Lohning, M. et al. (1998) Proc. Natl. Acad. Sci. USA 95:6930.
    7. Dinarello, C.A. (2005) Immunity 23:461.
    8. Chackerian, A.A. et al. (2007) J. Immunol. 179:2551.
     
  • Long Name:
    Interleukin 33
  • Entrez Gene IDs:
    90865 (Human); 77125 (Mouse)
  • Alternate Names:
    C9orf26; C9orf26chromosome 9 open reading frame 26 (NF-HEV); DKFZp586H0523; DVS27; DVS27-related protein; IL1F11; IL-1F11; IL33; IL-33; interleukin 33; Interleukin-1 family member 11; interleukin-33; NFHEV; NF-HEV; NF-HEVNFEHEV; Nuclear factor from high endothelial venules; RP11-575C20.2
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

14 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Aspirin-Exacerbated Respiratory Disease Involves a Cysteinyl Leukotriene-Driven IL-33-Mediated Mast Cell Activation Pathway.
    Authors: Liu T, Kanaoka Y, Barrett N, Feng C, Garofalo D, Lai J, Buchheit K, Bhattacharya N, Laidlaw T, Katz H, Boyce J
    J Immunol, 2016;195(8):3537-45.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  2. IL-33 is required for disposal of unnecessary cells during ovarian atresia through regulation of autophagy and macrophage migration.
    Authors: Wu J, Carlock C, Zhou C, Nakae S, Hicks J, Adams H, Lou Y
    J Immunol, 2015;194(5):2140-7.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IF
  3. Unique temporal and spatial expression patterns of IL-33 in ovaries during ovulation and estrous cycle are associated with ovarian tissue homeostasis.
    Authors: Carlock C, Wu J, Zhou C, Tatum K, Adams H, Tan F, Lou Y
    J Immunol, 2014;193(1):161-9.
  4. alpha-Galactosylceramide but not phenyl-glycolipids induced NKT cell anergy and IL-33-mediated myeloid-derived suppressor cell accumulation via upregulation of egr2/3.
    Authors: Huang J, Tsai Y, Chang Y, Wu J, Hung J, Lin K, Wong C, Yu A
    J Immunol, 2014;192(4):1972-81.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  5. Neutrophils prime a long-lived effector macrophage phenotype that mediates accelerated helminth expulsion.
    Authors: Chen, Fei, Wu, Wenhui, Millman, Ariel, Craft, Joshua F, Chen, Eunice, Patel, Nirav, Boucher, Jean L, Urban, Joseph F, Kim, Charles, Gause, William
    Nat Immunol, 2014;15(10):938-46.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  6. The alarmin IL-33 promotes regulatory T-cell function in the intestine.
    Authors: Schiering C, Krausgruber T, Chomka A, Frohlich A, Adelmann K, Wohlfert E, Pott J, Griseri T, Bollrath J, Hegazy A, Harrison O, Owens B, Lohning M, Belkaid Y, Fallon P, Powrie F
    Nature, 2014;513(7519):564-8.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  7. IL-33 targeting attenuates intestinal mucositis and enhances effective tumor chemotherapy in mice.
    Authors: Guabiraba R, Besnard A, Menezes G, Secher T, Jabir M, Amaral S, Braun H, Lima-Junior R, Ribeiro R, Cunha F, Teixeira M, Beyaert R, Graham G, Liew F
    Mucosal Immunol, 2014;7(5):1079-93.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  8. Apoptotic cell clearance by bronchial epithelial cells critically influences airway inflammation.
    Authors: Juncadella, Ignacio, Kadl, Alexandr, Sharma, Ashish K, Shim, Yun M, Hochreiter-Hufford, Amelia, Borish, Larry, Ravichandran, Kodi S
    Nature, 2013;493(7433):547-51.
    Species: Mouse
    Sample Type: In Vivo
    Application: Neut
  9. Interleukin-33 and alveolar macrophages contribute to the mechanisms underlying the exacerbation of IgE-mediated airway inflammation and remodelling in mice.
    Authors: Mizutani N, Nabe T, Yoshino S
    Immunology, 2013;139(2):205-18.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  10. Inducible IL-33 expression by mast cells is regulated by a calcium-dependent pathway.
    Authors: Hsu C, Bryce P
    J Immunol, 2012;189(7):3421-9.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  11. IL-33 attenuates EAE by suppressing IL-17 and IFN-gamma production and inducing alternatively activated macrophages.
    Authors: Jiang HR, Milovanovic M, Allan D, Niedbala W, Besnard AG, Fukada SY, Alves-Filho JC, Togbe D, Goodyear CS, Linington C, Xu D, Lukic ML, Liew FY
    Eur. J. Immunol., 2012;42(7):1804-14.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  12. Production and functions of IL-33 in the central nervous system.
    Authors: Yasuoka S, Kawanokuchi J, Parajuli B, Jin S, Doi Y, Noda M, Sonobe Y, Takeuchi H, Mizuno T, Suzumura A
    Brain Res., 2011;1385(0):8-17.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  13. IL-33 is produced by mast cells and regulates IgE-dependent inflammation.
    Authors: Hsu CL, Neilsen CV, Bryce PJ
    PLoS ONE, 2010;5(8):e11944.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  14. Induction of IL-33 expression and activity in central nervous system glia.
    Authors: Hudson CA, Christophi GP, Gruber RC, Wilmore JR, Lawrence DA, Massa PT
    J. Leukoc. Biol., 2008;84(3):631-43.
    Species: Mouse
    Sample Type: Cell Culture Supernates
    Application: ELISA Development
Expand to show all 14 Citations
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Average Rating: 4.5 (Based on 2 reviews)

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We have 2 review tested in 1 application: Western Blot.

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Images Ratings Applications Species Reviewed By Date Details
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 WB Mouse Anonymous 07/22/2016
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Summary

ApplicationWestern Blot
Sample TestedCell Lysates
SpeciesMouse
Western Blot Mouse IL‑33 Antibody MAB3626
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Very Good
 WB Anonymous 10/26/2015
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Western Blot Mouse IL‑33 Antibody MAB3626
Western Blot: Mouse IL‑33 Antibody [MAB3626]

Summary

ApplicationWestern Blot
Sample TestedSputum

Other Experimental Details

Other Experimental DetailsLoaded 5mcg protein/lane. Primary incubated O/N at 4 degrees with rocking.
Specificity: Reasonably specific
Sensitivity: Reasonably sensitive
Buffer: TBS-Tween, 5% non-fat milk
Dilution: 1:1000

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