Mouse IL-36 alpha /IL-1F6 Antibody

Catalog # Availability Size / Price Qty
MAB2297
MAB2297-SP
Product Details
Citations (1)
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Mouse IL-36 alpha /IL-1F6 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse IL‑36 alpha /IL‑1F6 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant mouse (rm) IL-1F5 and no cross-reactivity with recombinant human (rh) rhIL-1 alpha, rhIL-1 beta, rmIL-1F8, rhIL-1F6, 7, 9, 10, or rhIL-18 is observed.
Source
Monoclonal Rat IgG2A Clone # 275339
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant mouse IL‑36 alpha /IL‑1F6
Met1-His160
Accession # Q9JLA2
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse IL‑36 alpha /IL‑1F6 (Catalog # 2297-ML)
Blockade of Receptor-ligand Interaction
In a functional ELISA, 0.5-2 µg/mL of this antibody will block 50% of the binding of 5 μg/mL of Recombinant Mouse IL-1 Rrp2/IL-1 R6 Fc Chimera (Catalog # 2354-RP) to immobilized Recombinant Mouse IL‑36 alpha /IL‑1F6 (Catalog # 2297-ML) coated at 1 µg/mL (100 µL/well). At 10 μg/mL, this antibody will block >90% of the binding.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-36 alpha/IL-1F6

Mouse interleukin 1 family member #6 [IL-1F6; also named FIL-1 epsilon (epsilon)] is a member of the IL-1 family of proteins (1‑3). IL-1 family members include IL-1 beta, IL‑1 alpha, IL-1ra, IL-18 and IL-1F5 through F10 (4). All family members show a 12 beta -strand, beta -trefoil configuration, and all family members are believed to have arisen from a common ancestral gene that has undergone multiple duplications (4). IL-1F6 is synthesized as a 160 amino acid (aa) protein that contains no signal sequence, no prosegment and no potential N-linked glycosylation site(s) (2, 5). It appears to be actively secreted (1). When found in cell lysates, it presents as an 18 kDa monomer (2). Mouse to human, full length IL-1F6 has 54% aa identity. Within the family, IL-1F6 is 29% aa identical to IL-1ra, and 32%, 32%, 34%, 41%, and 28% aa identical to IL-1 beta, IL-1F5, F8, F9, and F10, respectively. Cells reported to express IL-1F6 include monocytes, B cells and T cells (1, 4). Notably, IL-1F6 is the only novel IL-1 family member found to be expressed on T-cells. The receptor for IL-1F6 is reported to be a combination of IL-1 Rrp2 and IL-1 RAcP (6). Recombinant IL‑1F6, along with IL-1F8 and IL-1F9, has been shown to act as an agonist by activating the pathway involving NF-kappa B and MAPK in an IL-1 Rrp2 dependent manner. This suggests that IL‑1F6 may signal in similar fashion to IL-1 and IL-18 in having a binding receptor which upon ligation, recruits a second receptor as a signaling component, forming an active heterodimeric receptor complex.

References
  1. Smith, D.E. et al. (2000) J. Biol. Chem. 275:1169.
  2. Kumar, S. et al. (2000) J. Biol. Chem. 275:10308.
  3. Nicklin, M.J.H. et al. (2002) Genomics. 79:718.
  4. Dunn, E. et al. (2001) Trends Immunol. 22:533.
  5. Debets, R. et al. (2001) J. Immunol. 167:1440.
  6. Towne, J.E. et al. (2004) J. Biol. Chem. 279:13677.
Long Name
Interleukin 36 alpha/Interleukin 1 Family 6
Entrez Gene IDs
27179 (Human); 54448 (Mouse)
Alternate Names
FIL1 epsilon; FIL1; FIL1(EPSILON); FIL1E; IL-1 epsilon; IL1(EPSILON); IL1E; IL-1E; IL-1F6 (FIL-1-epsilon); IL1F6; IL-1F6; IL36 alpha; IL-36 alpha; IL36A; interleukin 1 family, member 6 (epsilon); interleukin 1, epsilon; Interleukin 36, Alpha; Interleukin-1 epsilon; interleukin-1 family member 6; Interleukin-36 Alpha

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Citation for Mouse IL-36 alpha /IL-1F6 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. IL-36alpha exerts pro-inflammatory effects in the lungs of mice.
    Authors: Ramadas R, Ewart S, Iwakura Y, Medoff B, Levine A
    PLoS ONE, 2012-09-20;7(0):e45784.
    Species: Mouse
    Sample Types: Recombinant Protein
    Applications: Western Blot

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