Mouse IL-4 DuoSet ELISA

Catalog # Availability Size / Price Qty
DY404
DY404-05
Ancillary Products Available
Mouse IL-4 ELISA Standard Curve
1 Image
Product Details
Procedure
Citations (101)
FAQs
Supplemental Products
Reviews (5)

Mouse IL-4 DuoSet ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Volume Required
100 µL
Sufficient Materials
For five or fifteen 96-well plates*
Specificity
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant mouse IL-4. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Kit Content

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.

The components listed above may be purchased separately:

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or 0.05% Tween® 20 in PBS, pH 7.2-7.4

Reagent Diluent: (Catalog # DY995), or 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990)

Plate Sealers: ELISA Plate Sealers (Catalog # DY992)

Data Example

Mouse IL-4 ELISA Standard Curve

Product Datasheets

Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-4

IL-4 (Interleukin-4) is a cytokine that is primarily expressed by Th2-biased CD4+ T cells, mast cells, basophils, and eosinophils. It promotes cell proliferation, survival, and immunoglobulin class switch to IgG4 and IgE in human B cells, acquisition of the Th2 phenotype by naïve CD4+ T cells, priming and chemotaxis of mast cells, eosinophils, and basophils, and the proliferation and activation of epithelial cells. IL-4 plays a dominant role in the development of allergic inflammation and asthma. The type I receptor for IL-4, which is expressed on hematopoietic cells, is a heterodimer of the ligand binding IL-4 R? and the common ? chain (a shared subunit of the receptors for IL-2, -7, -9, -15, and -21). The type II receptor on nonhematopoietic cells consists of IL-4 R? and IL-13 R?1. The type II receptor also transduces IL-13 mediated signals.

Long Name:
Interleukin 4
Entrez Gene IDs:
3565 (Human); 16189 (Mouse); 287287 (Rat); 397225 (Porcine); 280824 (Bovine); 403785 (Canine); 574281 (Primate); 100302454 (Rabbit)
Alternate Names:
B cell growth factor 1; BCDF; B-cell stimulatory factor 1; BCGF1; BCGF-1; binetrakin; BSF1; BSF-1; IL4; IL-4; IL-4B_cell stimulatory factor 1; IL4E12; interleukin 4; interleukin-4; Lymphocyte stimulatory factor 1; MGC79402; Pitrakinra

Assay Procedure

GENERAL ELISA PROTOCOL

Plate Preparation

  1. Dilute the Capture Antibody to the working concentration in PBS without carrier protein. Immediately coat a 96-well microplate with 100 μL per well of the diluted Capture Antibody. Seal the plate and incubate overnight at room temperature.
  2. Aspirate each well and wash with Wash Buffer, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (400 μL) using a squirt bottle, manifold dispenser, or autowasher. Complete removal of liquid at each step is essential for good performance. After the last wash, remove any remaining Wash Buffer by aspirating or by inverting the plate and blotting it against clean paper towels.
  3. Block plates by adding 300 μL Reagent Diluent to each well. Incubate at room temperature for a minimum of 1 hour.
  4. Repeat the aspiration/wash as in step 2. The plates are now ready for sample addition.

Assay Procedure

  1. Add 100 μL of sample or standards in Reagent Diluent, or an appropriate diluent, per well. Cover with an adhesive strip and incubate 2 hours at room temperature.
  2. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  3. Add 100 μL of the Detection Antibody, diluted in Reagent Diluent, to each well. Cover with a new adhesive strip and incubate 2 hours at room temperature.
  4. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  5. Add 100 μL of the working dilution of Streptavidin-HRP to each well. Cover the plate and incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  6. Repeat the aspiration/wash as in step 2.
  7. Add 100 μL of Substrate Solution to each well. Incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  8. Add 50 μL of Stop Solution to each well. Gently tap the plate to ensure thorough mixing.
  9. Determine the optical density of each well immediately, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. If wavelength correction is not available, subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.

Citations for Mouse IL-4 DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

101 Citations: Showing 1 - 10
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  1. Strain-Specific Differences in House Dust Mite (Dermatophagoides farinae)-Induced Mouse Models of Allergic Rhinitis
    Authors: KI Lee, JS Bae, EH Kim, JH Kim, L Lyu, YJ Chung, JH Mo
    Clin Exp Otorhinolaryngol, 2020;0(0):.
    Species: Mouse
    Sample Types: cell culture supernatant
  2. Strongyloides venezuelensis-infection alters the profile of cytokines and liver inflammation in mice co-infected with Schistosoma mansoni
    Authors: MC de Rezende, JMP Moreira, LLM Fernandes, VF Rodrigues, D Negrão-Cor
    Cytokine, 2019;127(0):154931.
    Species: Mouse
    Sample Types: Tissue Homogenates
  3. SOCS2 modulates adipose tissue inflammation and expansion in mice
    Authors: CH Val, MC de Oliveir, DR Lacerda, A Barroso, NV Batista, Z Menezes-Ga, DRR de Assis, AT Cramer, F Brant, MM Teixeira, D Glória Sou, AM Ferreira, FS Machado
    J. Nutr. Biochem., 2019;76(0):108304.
    Species: Mouse
    Sample Types: Tissue Homogenates
  4. Non-hematopoietic STAT6 induces epithelial tight junction dysfunction and promotes intestinal inflammation and tumorigenesis
    Authors: Y Lin, B Li, X Yang, T Liu, T Shi, B Deng, Y Zhang, L Jia, Z Jiang, R He
    Mucosal Immunol, 2019;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  5. Anti-leishmanial activity of Brevinin 2R and its Lauric acid conjugate type against L. major: In vitro mechanism of actions and in vivo treatment potentials
    Authors: F Zahedifard, H Lee, JH No, M Salimi, N Seyed, A Asoodeh, S Rafati
    PLoS Negl Trop Dis, 2019;13(2):e0007217.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  6. Single-cell RNA-Seq analysis identifies a noncoding interleukin 4 (IL-4) RNA that post-transcriptionally up-regulates IL-4 production in T helper cells
    Authors: W Yin, Y Song, X Chang
    J. Biol. Chem., 2019;294(1):290-298.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  7. T cell microvilli constitute immunological synaptosomes that carry messages to antigen-presenting cells
    Authors: HR Kim, Y Mun, KS Lee, YJ Park, JS Park, JH Park, BN Jeon, CH Kim, Y Jun, YM Hyun, M Kim, SM Lee, CS Park, SH Im, CD Jun
    Nat Commun, 2018;9(1):3630.
    Species: Mouse
    Sample Types: Cell Lysates
  8. Repeated Allergen Exposure in A/J Mice Causes Steroid-Insensitive Asthma via a Defect in Glucocorticoid Receptor Bioavailability
    Authors: MF Serra, AC Cotias, CRR Pão, JB Daleprane, PB Jurgilas, GC Couto, EA Anjos-Valo, RSB Cordeiro, VF Carvalho, PMR Silva, MA Martins
    J. Immunol., 2018;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  9. Canonical PI3K? signaling in myeloid cells restricts Trypanosoma cruzi infection and dampens chagasic myocarditis
    Authors: MC Silva, M Davoli-Fer, TS Medina, R Sesti-Cost, GK Silva, CD Lopes, LE Cardozo, FN Gava, K Lyroni, FC Dias, AF Frade, M Baron, HI Nakaya, F Figueiredo, JC Alves-Filh, FQ Cunha, C Tsatsanis, C Chevillard, E Cunha-Neto, E Hirsch, JS Silva, TM Cunha
    Nat Commun, 2018;9(1):1513.
    Species: Mouse
    Sample Types: Serum
  10. Low dose of chlorine exposure exacerbates nasal and pulmonary allergic inflammation in mice
    Authors: IS de Genaro, FM de Almeida, DC Hizume-Kun, HT Moriya, RA Silva, JCG Cruz, RB Lopes, RF Righetti, R de Paula V, M Saiki, MA Martins, IFLC Tibério, FM Arantes-Co, BM Saraiva-Ro
    Sci Rep, 2018;8(1):12636.
    Species: Mouse
    Sample Types: Tissue Homogenates
  11. PrtA immunization fails to protect against pulmonary and invasive infection by Streptococcus pneumoniae
    Authors: CF Hsu, CH Hsiao, SF Tseng, JR Chen, YJ Liao, SJ Chen, CS Lin, HK Sytwu, YP Chuang
    Respir. Res., 2018;19(1):187.
    Species: Mouse
    Sample Types: Whole Cells
  12. Age-related cognitive impairment is associated with long-term neuroinflammation and oxidative stress in a mouse model of episodic systemic inflammation
    Authors: JC d'Avila, LD Siqueira, A Mazeraud, EP Azevedo, D Foguel, HC Castro-Far, T Sharshar, F Chrétien, FA Bozza
    J Neuroinflammation, 2018;15(1):28.
    Species: Mouse
    Sample Types: Tissue Homogenates
  13. Basophils are dispensable for the establishment of protective adaptive immunity against primary and challenge infection with the intestinal helminth parasite Strongyloides ratti
    Authors: M Reitz, ML Brunn, D Voehringer, M Breloer
    PLoS Negl Trop Dis, 2018;12(11):e0006992.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  14. The Plant Proteinase Inhibitor CrataBL Plays a Role in Controlling Asthma Response in Mice
    Authors: ASS Bortolozzo, APD Rodrigues, FM Arantes-Co, BM Saraiva-Ro, FCR de Souza, TR Brüggemann, MV de Brito, RDS Ferreira, MTDS Correia, PMG Paiva, CM Prado, EA Leick, MLV Oliva, MA Martins, VC Ruiz-Schut, RF Righetti, IFLC Tibério
    Biomed Res Int, 2018;2018(0):9274817.
    Species: Mouse
    Sample Types: Tissue Homogenates
  15. Brief Exposure of Skin to Near-Infrared Laser Modulates Mast Cell Function and Augments the Immune Response
    Authors: Y Kimizuka, W Katagiri, JJ Locascio, A Shigeta, Y Sasaki, M Shibata, K Morse, RF Sîrbulescu, M Miyatake, P Reeves, M Suematsu, J Gelfand, T Brauns, MC Poznansky, K Tsukada, S Kashiwagi
    J. Immunol., 2018;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  16. A novel multi-peptide subunit vaccine admixed with AddaVax adjuvant produces significant immunogenicity and protection against Proteus mirabilis urinary tract infection in mice model
    Authors: E Choubini, M Habibi, A Khorshidi, A Ghasemi, MR Asadi Kara, S Bouzari
    Mol. Immunol., 2018;96(0):88-97.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Standard
  17. Human Neutrophil Peptide 1 as immunotherapeutic agent against Leishmania infected BALB/c mice
    Authors: Z Abdossamad, N Seyed, F Zahedifard, T Taheri, Y Taslimi, H Montakhab-, A Badirzadeh, M Vasei, S Gharibzade, S Rafati
    PLoS Negl Trop Dis, 2017;11(12):e0006123.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  18. Erythroid Suppressor Cells Compromise Neonatal Immune Response against Bordetella pertussis
    Authors: G Dunsmore, N Bozorgmehr, C Delyea, P Koleva, A Namdar, S Elahi
    J. Immunol., 2017;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  19. Semiconductor diode laser device adjuvanting intradermal vaccine
    Authors: Y Kimizuka, JJ Callahan, Z Huang, K Morse, W Katagiri, A Shigeta, R Bronson, S Takeuchi, Y Shimaoka, MP Chan, Y Zeng, B Li, H Chen, RY Tan, C Dwyer, T Mulley, P Leblanc, C Goudie, J Gelfand, K Tsukada, T Brauns, MC Poznansky, D Bean, S Kashiwagi
    Vaccine, 2017;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  20. Therapeutic effect of human adipose-derived stem cells and their secretome in experimental diabetic pain
    Authors: AT Brini, G Amodeo, LM Ferreira, A Milani, S Niada, G Moschetti, S Franchi, E Borsani, LF Rodella, AE Panerai, P Sacerdote
    Sci Rep, 2017;7(1):9904.
    Species: Mouse
    Sample Types: Tissue Homogenates
  21. Inducing maternal inflammation promotes leptin production in offspring but does not improve allergic symptoms in a mouse model of allergic rhinitis
    Authors: A Imai, K Satoi, E Fujimoto, K Sato
    Heliyon, 2017;3(6):e00327.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  22. Mitochondrial CaMKII inhibition in airway epithelium protects against allergic asthma
    Authors: SC Sebag, OM Koval, JD Paschke, CJ Winters, OA Jaffer, R Dworski, FS Sutterwala, ME Anderson, IM Grumbach
    JCI Insight, 2017;2(3):e88297.
    Species: Mouse
    Sample Types: Tissue Homogenates
  23. Host DNA released by NETosis promotes rhinovirus-induced type-2 allergic asthma exacerbation
    Authors: M Toussaint, DJ Jackson, D Swieboda, A Guedán, TD Tsouroukts, YM Ching, C Radermecke, H Makrinioti, J Aniscenko, MR Edwards, R Solari, F Farnir, V Papayannop, F Bureau, T Marichal, SL Johnston
    Nat. Med., 2017;23(6):681-691.
    Species: Mouse
    Sample Types: Tissue Homogenates
  24. Suppression of colitis by adoptive transfer of helminth antigen-treated dendritic cells requires interleukin-4 receptor-� signaling
    Authors: CE Matisz, B Faz-L¢pez, E Thomson, A Al Rajabi, F Lopes, LI Terrazas, A Wang, KA Sharkey, DM McKay
    Sci Rep, 2017;7(0):40631.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  25. Experimental atopic dermatitis depends on IL-33R signaling via MyD88 in dendritic cells
    Authors: C Li, I Maillet, C Mackowiak, C Viala, F Di Padova, M Li, D Togbe, V Quesniaux, Y Lai, B Ryffel
    Cell Death Dis, 2017;8(4):e2735.
    Species: Mouse
    Sample Types: Tissue Homogenates
  26. Water-soluble chitosan inhibits nerve growth factor and attenuates allergic inflammation in mite allergen-induced allergic rhinitis
    Authors: PC Chen, MH Hsieh, P Wen-ShuoKu, HF Kao, CL Hsu, JY Wang
    J. Allergy Clin. Immunol., 2017;0(0):.
    Species: Mouse
    Sample Types: NLF
  27. Somatic extracts of Marshallagia marshalli downregulate the Th2 associated immune responses in ovalbumin-induced airway inflammation in BALB/c mice
    Authors: S Parande Sh, A Ebrahimby, A Dousty, M Maleki, A Movassaghi, H Borji, A Haghparast
    Parasit Vectors, 2017;10(1):233.
    Species: Mouse
    Sample Types: BALF
  28. Allergen-Induced IL-6 Regulates IL-9/IL-17A Balance in CD4+ T Cells in Allergic Airway Inflammation
    J Immunol, 2016;197(7):2653-64.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  29. IL-22 Restrains Tapeworm-Mediated Protection against Experimental Colitis via Regulation of IL-25 Expression
    Authors: JL Reyes, MR Fernando, F Lopes, G Leung, NL Mancini, CE Matisz, A Wang, DM McKay
    PLoS Pathog, 2016;12(4):e1005481.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  30. Editor's Highlight: Subvisible Aggregates of Immunogenic Proteins Promote a Th1-Type Response
    Authors: Jeremy P Derrick
    Toxicol. Sci., 2016;153(2):258-70.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  31. Testosterone-Mediated Endocrine Function and TH1/TH2 Cytokine Balance after Prenatal Exposure to Perfluorooctane Sulfonate: By Sex Status
    Int J Mol Sci, 2016;17(9):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  32. Repeated Activation of Lung Invariant NKT Cells Results in Chronic Obstructive Pulmonary Disease-Like Symptoms.
    Authors: Tsao C, Tsao P, Chen Y, Chuang Y
    PLoS ONE, 2016;11(1):e0147710.
    Species: Mouse
    Sample Types: BALF
  33. Alternatively Activated Mononuclear Phagocytes from the Skin Site of Infection and the Impact of IL-4R? Signalling on CD4+T Cell Survival in Draining Lymph Nodes after Repeated Exposure to Schistosoma mansoni Cercariae
    PLoS Negl Trop Dis, 2016;10(8):e0004911.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  34. Comparison of potential protection conferred by three immunization strategies (protein/protein, DNA/DNA, and DNA/protein) against Brucella infection using Omp2b in BALB/c Mice
    Authors: Saeid Bouzari
    Vet. Microbiol., 2016;197(0):47-52.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  35. The soluble guanylyl cyclase activator BAY 60-2770 inhibits murine allergic airways inflammation and human eosinophil chemotaxis
    Authors: Edson Antunes
    Pulm Pharmacol Ther, 2016;0(0):.
    Species: Mouse
    Sample Types: BALF
  36. Impact of Histone H1 on the Progression of Allergic Rhinitis and Its Suppression by Neutralizing Antibody in Mice
    Authors: T Nakano, R Kamei, T Fujimura, Y Takaoka, A Hori, CY Lai, KC Chiang, Y Shimada, N Ohmori, T Goto, K Ono, CL Chen, S Goto, S Kawamoto
    PLoS ONE, 2016;11(4):e0153630.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  37. Acid-Sensing Ion Channel 1a Contributes to Airway Hyperreactivity in Mice
    PLoS ONE, 2016;11(11):e0166089.
    Species: Mouse
    Sample Types: BALF
  38. CD4+ T cell hyporesponsiveness after repeated exposure to Schistosoma mansoni larvae is dependent upon interleukin-10.
    Authors: Prendergast C, Sanin D, Cook P, Mountford A
    Infect Immun, 2015;83(4):1418-30.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  39. Prostaglandin E2 Production and T Cell Function in Mouse Adenovirus Type 1 Infection following Allogeneic Bone Marrow Transplantation.
    Authors: McCarthy M, Procario M, Wilke C, Moore B, Weinberg J
    PLoS ONE, 2015;10(9):e0139235.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  40. Superior Suppressive Capacity of Skin Tregs Compared with Lung Tregs in a Model of Epicutaneous Priming.
    Authors: Mahapatra S, Albrecht M, Baru A, Sparwasser T, Herrick C, Dittrich A
    J Invest Dermatol, 2015;135(10):2418-26.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  41. CD252 regulates mast cell mediated, CD1d-restricted NKT-cell activation in mice.
    Authors: Gonzalez Roldan N, Orinska Z, Ewers H, Bulfone-Paus S
    Eur J Immunol, 2015;46(2):432-9.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  42. Intestinal helminths regulate lethal acute graft-versus-host disease and preserve the graft-versus-tumor effect in mice.
    Authors: Li Y, Chen H, Bannick N, Henry M, Holm A, Metwali A, Urban J, Rothman P, Weiner G, Blazar B, Elliott D, Ince M
    J Immunol, 2015;194(3):1011-20.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  43. SLy2 controls the antibody response to pneumococcal vaccine through an IL-5Ralpha-dependent mechanism in B-1 cells.
    Authors: Schmitt F, Schall D, Bucher K, Schindler T, Hector A, Biedermann T, Zemlin M, Hartl D, Beer-Hammer S
    Eur J Immunol, 2015;45(1):60-70.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  44. Signaling via tumor necrosis factor receptor 1 but not Toll-like receptor 2 contributes significantly to hydrosalpinx development following Chlamydia muridarum infection.
    Authors: Dong X, Liu Y, Chang X, Lei L, Zhong G
    Infect Immun, 2014;82(5):1833-9.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  45. A ThPOK-LRF transcriptional node maintains the integrity and effector potential of post-thymic CD4+ T cells.
    Authors: Vacchio M, Wang L, Bouladoux N, Carpenter A, Xiong Y, Williams L, Wohlfert E, Song K, Belkaid Y, Love P, Bosselut R
    Nat Immunol, 2014;15(10):947-56.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  46. Lifetime-dependent effects of bisphenol A on asthma development in an experimental mouse model.
    Authors: Petzold, Susanne, Averbeck, Marco, Simon, Jan C, Lehmann, Irina, Polte, Tobias
    PLoS ONE, 2014;9(6):e100468.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  47. IL-17 contributes to neutrophil recruitment but not to control of viral replication during acute mouse adenovirus type 1 respiratory infection.
    Authors: McCarthy M, Zhu L, Procario M, Weinberg J
    Virology, 2014;456(0):259-67.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  48. The glycosylated Rv1860 protein of mycobacterium tuberculosis inhibits dendritic cell mediated TH1 and TH17 polarization of T cells and abrogates protective immunity conferred by BCG.
    Authors: Satchidanandam V, Kumar N, Jumani R, Challu V, Elangovan S, Khan N
    PLoS Pathog, 2014;10(6):e1004176.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  49. IL-25 and type 2 innate lymphoid cells induce pulmonary fibrosis.
    Authors: Hams, Emily, Armstrong, Michelle, Barlow, Jillian, Saunders, Sean P, Schwartz, Christia, Cooke, Gordon, Fahy, Ruairi J, Crotty, Thomas B, Hirani, Nikhil, Flynn, Robin J, Voehringer, David, McKenzie, Andrew N, Donnelly, Seamas C, Fallon, Padraic
    Proc Natl Acad Sci U S A, 2014;111(1):367-72.
    Species: Transgenic Rat
    Sample Types: Tissue Homogenates
  50. Foxp3(+) regulatory T cells delay expulsion of intestinal nematodes by suppression of IL-9-driven mast cell activation in BALB/c but not in C57BL/6 mice.
    Authors: Blankenhaus B, Reitz M, Brenz Y, Eschbach M, Hartmann W, Haben I, Sparwasser T, Huehn J, Kuhl A, Feyerabend T, Rodewald H, Breloer M
    PLoS Pathog, 2014;10(2):e1003913.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  51. An iron-acquisition-deficient mutant of Corynebacterium pseudotuberculosis efficiently protects mice against challenge.
    Authors: Ribeiro D, Rocha F, Leite K, Soares S, Silva A, Portela R, Meyer R, Miyoshi A, Oliveira S, Azevedo V, Dorella F
    Vet Res, 2014;45(0):28.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  52. Delineating the role of histamine-1- and -4-receptors in a mouse model of Th2-dependent antigen-specific skin inflammation.
    Authors: Mahapatra S, Albrecht M, Behrens B, Jirmo A, Behrens G, Hartwig C, Neumann D, Raap U, Bahre H, Herrick C, Dittrich A
    PLoS ONE, 2014;9(2):e87296.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  53. Genetic deletion of the HIF-1alpha isoform I.1 in T cells enhances antibacterial immunity and improves survival in a murine peritonitis model.
    Authors: Georgiev P, Belikoff B, Hatfield S, Ohta A, Sitkovsky M, Lukashev D
    Eur J Immunol, 2013;43(3):655-66.
    Species: Mouse
    Sample Types: Serum
  54. Nanogel-based PspA intranasal vaccine prevents invasive disease and nasal colonization by Streptococcus pneumoniae.
    Authors: Kong, Il Gyu, Sato, Ayuko, Yuki, Yoshikaz, Nochi, Tomonori, Takahashi, Haruko, Sawada, Shinichi, Mejima, Mio, Kurokawa, Shiho, Okada, Kazunari, Sato, Shintaro, Briles, David E, Kunisawa, Jun, Inoue, Yusuke, Yamamoto, Masafumi, Akiyoshi, Kazunari, Kiyono, Hiroshi
    Infect Immun, 2013;81(5):1625-34.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  55. Bifidobacterium longum alleviates dextran sulfate sodium-induced colitis by suppressing IL-17A response: involvement of intestinal epithelial costimulatory molecules.
    Authors: Miyauchi, Eiji, Ogita, Tasuku, Miyamoto, Junki, Kawamoto, Seiji, Morita, Hidetosh, Ohno, Hiroshi, Suzuki, Takuya, Tanabe, Soichi
    PLoS ONE, 2013;8(11):e79735.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  56. Rac2-deficiency leads to exacerbated and protracted colitis in response to Citrobacter rodentium infection.
    Authors: Fattouh R, Guo C, Lam G, Gareau M, Ngan B, Glogauer M, Muise A, Brumell J
    PLoS ONE, 2013;8(4):e61629.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  57. Immune Modulation Mediated by Cryptococcal Laccase Promotes Pulmonary Growth and Brain Dissemination of Virulent Cryptococcus neoformans in Mice.
    Authors: Qiu Y, Davis M, Dayrit J, Hadd Z, Meister D, Osterholzer J, Williamson P, Olszewski M
    PLoS ONE, 2012;7(10):e47853.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  58. Control of allergen-induced inflammation and hyperresponsiveness by the metalloproteinase ADAMTS-12.
    Authors: Paulissen G, El Hour M, Rocks N, Gueders M, Bureau F, Foidart J, Lopez-Otin C, Noel A, Cataldo D
    J Immunol, 2012;189(8):4135-43.
    Species: Mouse
    Sample Types: Tissue Homogenates
  59. Role of Toll interleukin-1 receptor (IL-1R) 8, a negative regulator of IL-1R/Toll-like receptor signaling, in resistance to acute Pseudomonas aeruginosa lung infection.
    Authors: Veliz Rodriguez T, Moalli F, Polentarutti N, Paroni M, Bonavita E, Anselmo A, Nebuloni M, Mantero S, Jaillon S, Bragonzi A, Mantovani A, Riva F, Garlanda C
    Infect. Immun., 2012;80(1):100-9.
    Species: Mouse
    Sample Types: Serum
  60. Schistosome-derived omega-1 drives Th2 polarization by suppressing protein synthesis following internalization by the mannose receptor.
    J. Exp. Med., 2012;209(10):1753-67, S1.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  61. TLR9 and MyD88 are crucial for the development of protective immunity to malaria.
    Authors: Gowda NM, Wu X, Gowda DC
    J. Immunol., 2012;188(10):5073-85.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  62. Api6/AIM/Sp{alpha}/CD5L Overexpression in Alveolar Type II Epithelial Cells Induces Spontaneous Lung Adenocarcinoma.
    Authors: Li Y, Qu P, Wu L, Li B, Du H, Yan C
    Cancer Res., 2011;71(16):5488-99.
    Species: Mouse
    Sample Types: Serum
  63. The Therapeutic Potential of the Humoral Pattern Recognition Molecule PTX3 in Chronic Lung Infection Caused by Pseudomonas aeruginosa.
    Authors: Moalli F, Paroni M, Veliz Rodriguez T, Riva F, Polentarutti N, Bottazzi B, Valentino S, Mantero S, Nebuloni M, Mantovani A, Bragonzi A, Garlanda C
    J. Immunol., 2011;186(9):5425-34.
    Species: Mouse
    Sample Types: Tissue Homogenates
  64. IL-33-responsive lineage- CD25+ CD44(hi) lymphoid cells mediate innate type 2 immunity and allergic inflammation in the lungs.
    Authors: Bartemes KR, Iijima K, Kobayashi T, Kephart GM, McKenzie AN, Kita H
    J. Immunol., 2011;188(3):1503-13.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  65. T cell-derived Act1 is necessary for IL-25-mediated Th2 responses and allergic airway inflammation.
    Authors: Swaidani S, Bulek K, Kang Z
    J. Immunol., 2011;187(6):3155-64.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  66. Alpha-Galactosylceramide-induced airway eosinophilia is mediated through the activation of NKT cells.
    Authors: Chuang YH, Wang TC, Jen HY, Yu AL, Chiang BL
    J. Immunol., 2010;186(8):4687-92.
    Species: Mouse
    Sample Types: BALF
  67. Th17 cells are the dominant T cell subtype primed by Shigella flexneri mediating protective immunity.
    Authors: Sellge G, Magalhaes JG, Konradt C, Fritz JH, Salgado-Pabon W, Eberl G, Bandeira A, Di Santo JP, Sansonetti PJ, Phalipon A
    J. Immunol., 2010;184(4):2076-85.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  68. A critical role for C5L2 in the pathogenesis of experimental allergic asthma.
    Authors: Zhang X, Schmudde I, Laumonnier Y, Pandey MK, Clark JR, Konig P, Gerard NP, Gerard C, Wills-Karp M, Kohl J
    J. Immunol., 2010;185(11):6741-52.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  69. Mucosal delivery of human papillomavirus pseudovirus-encapsidated plasmids improves the potency of DNA vaccination.
    Authors: Graham BS, Kines RC, Corbett KS, Nicewonger J, Roberts JN, Schiller JT, Buck CB
    Mucosal Immunol, 2010;3(5):475-86.
    Species: Mouse
    Sample Types: BALF
  70. Effects of umbelliferone in a murine model of allergic airway inflammation.
    Authors: Vasconcelos JF, Teixeira MM, Barbosa-Filho JM, Agra MF, Nunes XP, Giulietti AM, Ribeiro-Dos-Santos R, Soares MB
    Eur. J. Pharmacol., 2009;609(1):126-31.
    Species: Mouse
    Sample Types: BALF
  71. A chlamydial type III-secreted effector protein (Tarp) is predominantly recognized by antibodies from humans infected with Chlamydia trachomatis and induces protective immunity against upper genital tract pathologies in mice.
    Authors: Wang J, Chen L, Chen F, Zhang X, Zhang Y, Baseman J, Perdue S, Yeh IT, Shain R, Holland M, Bailey R, Mabey D, Yu P, Zhong G
    Vaccine, 2009;27(22):2967-80.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  72. The Decoy Receptor 3 (DcR3, TNFRSF6B) suppresses Th17 immune responses and is abundant in human cerebrospinal fluid.
    Authors: Mueller AM, Pedre X, Killian S, David M, Steinbrecher A
    J. Neuroimmunol., 2009;209(1):57-64.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  73. Mucosal immunity in mice induced by orally administered transgenic rice.
    Authors: Zhang X, Yuan Z, Duan Q, Zhu H, Yu H, Wang Q
    Vaccine, 2009;27(10):1596-600.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  74. Central role of MyD88-dependent dendritic cell maturation and proinflammatory cytokine production to control Brucella abortus infection.
    Authors: Macedo GC, Magnani DM, Carvalho NB, Bruna-Romero O, Gazzinelli RT, Oliveira SC
    J. Immunol., 2008;180(2):1080-7.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  75. Transforming growth factor-beta regulates house dust mite-induced allergic airway inflammation but not airway remodeling.
    Authors: Fattouh R, Midence NG, Arias K, Johnson JR, Walker TD, Goncharova S, Souza KP, Gregory RC, Lonning S, Gauldie J, Jordana M
    Am. J. Respir. Crit. Care Med., 2008;177(6):593-603.
    Species: Mouse
    Sample Types: BALF
  76. Identification of cardiac troponin I sequence motifs leading to heart failure by induction of myocardial inflammation and fibrosis.
    Authors: Kaya Z, Goser S, Buss SJ, Leuschner F, Ottl R, Li J, Volkers M, Zittrich S, Pfitzer G, Rose NR, Katus HA
    Circulation, 2008;118(20):2063-72.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  77. Amelioration of experimental autoimmune encephalomyelitis in IL-4Ralpha-/- mice implicates compensatory up-regulation of Th2-type cytokines.
    Authors: Gaupp S, Cannella B, Raine CS
    Am. J. Pathol., 2008;173(1):119-29.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  78. Adenosine A2A receptor activation inhibits T helper 1 and T helper 2 cell development and effector function.
    Authors: Csoka B, Himer L, Selmeczy Z, Vizi ES, Pacher P, Ledent C, Deitch EA, Spolarics Z, Nemeth ZH, Hasko G
    FASEB J., 2008;22(10):3491-9.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  79. Streptococcus equi antigens adsorbed onto surface modified poly-epsilon-caprolactone microspheres induce humoral and cellular specific immune responses.
    Authors: Florindo HF, Pandit S, Goncalves LM, Alpar HO, Almeida AJ
    Vaccine, 2008;26(33):4168-77.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  80. VAMP-8 segregates mast cell-preformed mediator exocytosis from cytokine trafficking pathways.
    Authors: Tiwari N, Wang CC, Brochetta C, Ke G, Vita F, Qi Z, Rivera J, Soranzo MR, Zabucchi G, Hong W, Blank U
    Blood, 2008;111(7):3665-74.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  81. Lipoxin A4 stable analogs reduce allergic airway responses via mechanisms distinct from CysLT1 receptor antagonism.
    Authors: Levy BD, Lukacs NW, Berlin AA, Schmidt B, Guilford WJ, Serhan CN, Parkinson JF
    FASEB J., 2007;21(14):3877-84.
    Species: Mouse
    Sample Types: Tissue Homogenates
  82. Nod1-mediated innate immune recognition of peptidoglycan contributes to the onset of adaptive immunity.
    Authors: Fritz JH, Le Bourhis L, Sellge G, Magalhaes JG, Fsihi H, Kufer TA, Collins C, Viala J, Ferrero RL, Girardin SE, Philpott DJ
    Immunity, 2007;26(4):445-59.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  83. Lactoferrin enhanced efficacy of the BCG vaccine to generate host protective responses against challenge with virulent Mycobacterium tuberculosis.
    Authors: Hwang SA, Wilk KM, Budnicka M, Olsen M, Bangale YA, Hunter RL, Kruzel ML, Actor JK
    Vaccine, 2007;25(37):6730-43.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  84. The related adaptors, adaptor in lymphocytes of unknown function X and Rlk/Itk-binding protein, have nonredundant functions in lymphocytes.
    Authors: Perchonock CE, Pajerowski AG, Nguyen C, Shapiro MJ, Shapiro VS
    J. Immunol., 2007;179(3):1768-75.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  85. Differing effects of clarithromycin and azithromycin on cytokine production by murine dendritic cells.
    Authors: Sugiyama K, Shirai R, Mukae H, Ishimoto H, Nagata T, Sakamoto N, Ishii H, Nakayama S, Yanagihara K, Mizuta Y, Kohno S
    Clin. Exp. Immunol., 2007;147(3):540-6.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  86. CD40L disruption enhances Abeta vaccine-mediated reduction of cerebral amyloidosis while minimizing cerebral amyloid angiopathy and inflammation.
    Authors: Obregon D, Hou H, Bai Y, Nikolic WV, Mori T, Luo D, Zeng J, Ehrhart J, Fernandez F, Morgan D, Giunta B, Town T, Tan J
    Neurobiol. Dis., 2007;29(2):336-53.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  87. An alternative pathway of NF-kappaB activation results in maturation and T cell priming activity of dendritic cells overexpressing a mutated IkappaBalpha.
    Authors: Moore F, Buonocore S, Aksoy E, Ouled-Haddou N, Goriely S, Lazarova E, Paulart F, Heirman C, Vaeremans E, Thielemans K, Goldman M, Flamand V
    J. Immunol., 2007;178(3):1301-11.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  88. Pulmonary distribution, regulation, and functional role of Trk receptors in a murine model of asthma.
    Authors: Nassenstein C, Dawbarn D, Pollock K, Allen SJ, Erpenbeck VJ, Spies E, Krug N, Braun A
    J. Allergy Clin. Immunol., 2006;118(3):597-605.
    Species: Mouse
    Sample Types: BALF
  89. Hierarchical suppression of asthma-like responses by mucosal tolerance.
    Authors: Keller AC, Mucida D, Gomes E, Faquim-Mauro E, Faria AM, Rodriguez D, Russo M
    J. Allergy Clin. Immunol., 2006;117(2):283-90.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  90. Immunity induced with a Salmonella enterica serovar Enteritidis live vaccine is regulated by Th1-cell-dependent cellular and humoral effector mechanisms in susceptible BALB/c mice.
    Authors: Lehmann J, Springer S, Werner CE, Lindner T, Bellmann S, Straubinger RK, Selbitz HJ, Alber G
    Vaccine, 2006;24(22):4779-93.
    Species: Mouse
    Sample Types: Serum
  91. Exogenous IL-12 suppresses experimental autoimmune encephalomyelitis (EAE) by tuning IL-10 and IL-5 levels in an IFN-gamma-dependent way.
    Authors: Berghmans N, Dillen C, Heremans H
    J. Neuroimmunol., 2006;176(1):63-75.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  92. The effects of dehydroepiandrosterone (DHEA) on in vitro spleen cell proliferation and cytokine production.
    Authors: Powell JM, Sonnenfeld G
    J. Interferon Cytokine Res., 2006;26(1):34-9.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  93. Comparative immunomodulatory properties of a chitosan-MDP adjuvant combination following intranasal or intramuscular immunisation.
    Authors: Moschos SA, Bramwell VW, Somavarapu S, Alpar HO
    Vaccine, 2005;23(16):1923-30.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  94. Subcellular fractions of Brucella ovis distinctively induce the production of interleukin-2, interleukin-4, and interferon-gamma in mice.
    Authors: Salas-Tellez E, Nunez del Arco A, Tenorio V, Diaz-Aparicio E, de la Garza M, Suarez-Guemes F
    Can. J. Vet. Res., 2005;69(1):53-7.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  95. Leukemia inhibitory factor is linked to regulatory transplantation tolerance.
    Authors: Metcalfe SM, Watson TJ, Shurey S, Adams E, Green CJ
    Transplantation, 2005;79(6):726-30.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  96. The IgE adjuvant effect of particles: characterisation of the primary cellular response in the draining lymph node.
    Authors: Nygaard UC, Ormstad H, Aase A, Lovik M
    Toxicology, 2005;206(2):181-93.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  97. Leukaemia inhibitory factor (LIF) is functionally linked to axotrophin and both LIF and axotrophin are linked to regulatory immune tolerance.
    Authors: Metcalfe SM, Muthukumarana PA, Thompson HL, Haendel MA, Lyons GE
    FEBS Lett., 2005;579(3):609-14.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  98. Active hexose correlated compound enhances the immune function of mice in the hindlimb-unloading model of spaceflight conditions.
    Authors: Aviles H, Belay T, Vance M, Sun B, Sonnenfeld G
    J. Appl. Physiol., 2004;97(4):1437-44.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  99. Comparison of immunogenicities of recombinant Plasmodium vivax merozoite surface protein 1 19- and 42-kiloDalton fragments expressed in Escherichia coli.
    Authors: Sachdeva S, Ahmad G, Malhotra P, Mukherjee P, Chauhan VS
    Infect. Immun., 2004;72(10):5775-82.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  100. Transcutaneous immunization with cholera toxin B subunit adjuvant suppresses IgE antibody responses via selective induction of Th1 immune responses.
    Authors: Anjuere F, George-Chandy A, Audant F, Rousseau D, Holmgren J, Czerkinsky C
    J. Immunol., 2003;170(3):1586-92.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  101. Intracellular thiols contribute to Th2 function via a positive role in IL-4 production.
    Authors: Monick MM, Samavati L, Butler NS, Mohning M, Powers LS, Yarovinsky T, Spitz DR, Hunninghake GW
    J. Immunol., 2003;171(10):5107-15.
    Species: Mouse
    Sample Types: Cell Culture Supernates

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Mouse IL-4 DuoSet ELISA
By Anonymous on 07/23/2020
Application: Sample Tested: Serum

Mouse IL-4 DuoSet ELISA
By Anonymous on 06/18/2020
Application: Sample Tested: Mouse spleenocytes

We used this kit to detect the IL-4 production from mice immunized by our vaccine candidate, the kit really works well and convenient, and cheaper compared to other kits. We highly recommended it


Mouse IL-4 DuoSet ELISA
By Anonymous on 11/13/2019
Application: Sample Tested: Serum

Mouse IL-4 DuoSet ELISA
By Anonymous on 06/03/2016
Application: Sample Tested: Serum

I have used DuoSet ELISAs for years and appreciate the simplicity and ease of use for the kits. We don't perform ELISAs at the same rate we used to, but when we needed to do a quick IL-4 test of some sera. I purchased this kit, the accessory kit and was able to do all I needed quickly. I recommend DuoSet ELISA kits to everyone.


Mouse IL-4 DuoSet ELISA, 15 Plate
By Anonymous on 10/26/2015
Application: Sample Tested: In vitro derived lymphocytes

Specificity: Specific
Sensitivity: Sensitive
Buffer: 1% BSA in PBS, pH 7.2 - 7.4, filtered
Dilution: 1/180