Mouse IL-4 ELISpot Kit

Catalog # Availability Size / Price Qty
XEL404
EL404

15% Off Limited-time Savings on Your Reagents, Assays, and Kits
Now through September 27th, you can save 15% on Bio-Techne reagents, assays, and kits (including Novus, Tocris, and R&D Systems). Use promotion code SAVE15 at checkout on orders over $1,000. See terms and conditions for offer details.

R&D Systems ELISpot and FluoroSpot Kits
1 Image
Product Details
Citations (10)
FAQs
Supplemental Products
Reviews

Mouse IL-4 ELISpot Kit Summary

Assay Type
Quantitative Sandwich ELISA
Format
96-well PVDF-backed microplate
Assay Length
3 hours 15 mins to 4 hours 30 mins*
Sample Type
Whole Cells
Specificity
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.


PRODUCT SUMMARY
ELISpot kits are highly sensitive, microplate-based assays for the detection of cytokine secreting cells. This kit is designed for the detection and enumeration of mouse IL-4. Complete ELISpot kits are ready-to-run and require no assay development or refinement.

This ELISpot assay employs a capture antibody specific for mouse IL-4, pre-coated onto a PVDF-backed microplate. Appropriately stimulated cells are pipetted directly into the wells and the immobilized antibody in the immediate vicinity of the secreting cells binds secreted mouse IL-4. Following wash steps and incubation with a biotinylated detection antibody, alkaline-phosphatase conjugated to streptavidin is added. Unbound enzyme is subsequently removed by washing and a substrate solution (BCIP/NBT) is added. A blue-black colored precipitate forms at the sites of cytokine localization and appears as spots, with each individual spot representing an individual mouse IL-4 secreting cell. The spots can be counted with an automated ELISpot reader system or manually using a stereomicroscope.


PRODUCT FEATURES

  • Detect and quantitate individual cells secreting mouse IL-4
  • High sensitivity - ELISpot assays can measure responses with frequencies well below 1 in 100,000 cells
  • No in vitro expansion of cells required
  • High-throughput - ELISpot assays use only a small number of primary cells


KIT CONTENTS

  • Mouse IL-4 Microplate
  • Biotinylated Detection Antibody
  • Streptavidin conjugated to Alkaline Phosphatase
  • Dilution Buffers
  • Wash Buffer Concentrate
  • BCIP/NBT Chromogen
  • Mouse IL-4 Positive Control

OTHER REAGENTS REQUIRED

  • Pipettes and pipette tips
  • Deionized or distilled water
  • Squirt bottle, manifold dispenser, or automated microplate washer
  • 500 mL graduated cylinder
  • 37 °C CO2 incubator
  • Sterile culture media
  • Dissection microscope or an automated ELISpot reader

Product Datasheets

You must select a language.

x

Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-4

IL-4 (Interleukin-4) is a cytokine that is primarily expressed by Th2-biased CD4+ T cells, mast cells, basophils, and eosinophils. It promotes cell proliferation, survival, and immunoglobulin class switch to IgG4 and IgE in human B cells, acquisition of the Th2 phenotype by naïve CD4+ T cells, priming and chemotaxis of mast cells, eosinophils, and basophils, and the proliferation and activation of epithelial cells. IL-4 plays a dominant role in the development of allergic inflammation and asthma. The type I receptor for IL-4, which is expressed on hematopoietic cells, is a heterodimer of the ligand binding IL-4 R? and the common ? chain (a shared subunit of the receptors for IL-2, -7, -9, -15, and -21). The type II receptor on nonhematopoietic cells consists of IL-4 R? and IL-13 R?1. The type II receptor also transduces IL-13 mediated signals.

Long Name:
Interleukin 4
Entrez Gene IDs:
3565 (Human); 16189 (Mouse); 287287 (Rat); 397225 (Porcine); 280824 (Bovine); 403785 (Canine); 574281 (Primate); 100302454 (Rabbit)
Alternate Names:
B cell growth factor 1; BCDF; B-cell stimulatory factor 1; BCGF1; BCGF-1; binetrakin; BSF1; BSF-1; IL4; IL-4; IL-4B_cell stimulatory factor 1; IL4E12; interleukin 4; interleukin-4; Lymphocyte stimulatory factor 1; MGC79402; pitrakinra
⚠ WARNING: This product can expose you to chemicals including methanol, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov.

Citations for Mouse IL-4 ELISpot Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
Filter your results:

Filter by:

  1. A two-adjuvant multiantigen candidate vaccine induces superior protective immune responses against SARS-CoV-2 challenge
    Authors: W Jiang, L Shi, L Cai, X Wang, J Li, H Li, J Liang, Q Gu, G Ji, J Li, L Liu, M Sun
    Cell Reports, 2021;37(11):110112.
  2. HIV-1 adenoviral vector vaccines expressing multi-trimeric BAFF and 4-1BBL enhance T cell mediated anti-viral immunity.
    Authors: Kanagavelu, Saravana, Termini, James M, Gupta, Sachin, Raffa, Francesc, Fuller, Katherin, Rivas, Yaelis, Philip, Sakhi, Kornbluth, Richard, Stone, Geoffrey
    PLoS ONE, 2014;9(2):e90100.
    Species: Mouse
    Sample Types: Whole Cells
  3. Elongated TCR alpha chain CDR3 favors an altered CD4 cytokine profile.
    Authors: Reynolds C, Chong D, Raynsford E, Quigley K, Kelly D, Llewellyn-Hughes J, Altmann D, Boyton R
    BMC Biol, 2014;12(0):32.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  4. Production and immunogenicity of chimeric virus-like particles containing the spike glycoprotein of infectious bronchitis virus.
    Authors: Lv L, Li X, Liu G, Li R, Liu Q, Shen H, Wang W, Xue C, Cao Y
    J Vet Sci, 2014;15(2):209-16.
    Species: Mouse
    Sample Types: Whole Cells
  5. The Shc family protein adaptor, Rai, acts as a negative regulator of Th17 and Th1 cell development.
    Authors: Savino, Maria Te, Ulivieri, Cristina, Emmi, Giacomo, Prisco, Domenico, De Falco, Giulia, Ortensi, Barbara, Beccastrini, Enrico, Emmi, Lorenzo, Pelicci, Giuliana, D'Elios, Mario M, Baldari, Cosima T
    J Leukoc Biol, 2013;93(4):549-59.
    Species: Mouse
    Sample Types: Whole Cells
  6. A VLP vaccine induces broad-spectrum cross-protective antibody immunity against H5N1 and H1N1 subtypes of influenza A virus.
    Authors: Wu, Chia-Yin, Yeh, Yi-Chun, Chan, Jia-Tsro, Yang, Yu-Chih, Yang, Ji-Rong, Liu, Ming-Tsa, Wu, Ho-Sheng, Hsiao, Pei-Wen
    PLoS ONE, 2012;7(8):e42363.
    Species: Mouse
    Sample Types: Whole Cells
  7. Critical role of donor tissue expression of programmed death ligand-1 in regulating cardiac allograft rejection and vasculopathy.
    Authors: Yang J, Popoola J, Khandwala S, Vadivel N, Vanguri V, Yuan X, Dada S, Guleria I, Tian C, Ansari MJ, Shin T, Yagita H, Azuma M, Sayegh MH, Chandraker A
    Circulation, 2008;117(5):660-9.
    Species: Mouse
    Sample Types: Whole Cells
  8. TH17 cells mediate steroid-resistant airway inflammation and airway hyperresponsiveness in mice.
    Authors: McKinley L, Alcorn JF, Peterson A, Dupont RB, Kapadia S, Logar A, Henry A, Irvin CG, Piganelli JD, Ray A, Kolls JK
    J. Immunol., 2008;181(6):4089-97.
    Species: Mouse
    Sample Types: Whole Cells
  9. Nitric oxide mediates T cell cytokine production and signal transduction in histidine decarboxylase knockout mice.
    Authors: Koncz A, Pasztoi M, Mazan M, Fazakas F, Buzas E, Falus A, Nagy G
    J. Immunol., 2007;179(10):6613-9.
    Species: Mouse
    Sample Types: Whole Cells
  10. Immunopharmacological and antitumor effects of second-generation immunomodulatory oligonucleotides containing synthetic CpR motifs.
    Authors: Wang D, Li Y, Yu D, Song SS, Kandimalla ER, Agrawal S
    Int. J. Oncol., 2004;24(4):901-8.
    Species: Mouse
    Sample Types: Whole Cells

FAQs

No product specific FAQs exist for this product, however you may

View all ELISpot and FluoroSpot Kit FAQs

Supplemental ELISA Products

Reviews for Mouse IL-4 ELISpot Kit

There are currently no reviews for this product. Be the first to review Mouse IL-4 ELISpot Kit and earn rewards!

Have you used Mouse IL-4 ELISpot Kit?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review