Mouse Integrin  alpha 5/CD49e Antibody

Catalog # Availability Size / Price Qty
AF1754
AF1754-SP
Product Details
Citations (2)
FAQs
Supplemental Products
Reviews

Mouse Integrin  alpha 5/CD49e Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Integrin alpha 5/CD49e in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross‑reactivity with recombinant human (rh) Integrin alpha 5 is observed, 15% cross‑reactivity with rhIntegrin alpha 2 and rhIntegrin alpha M is observed, and 5% cross‑reactivity with rhIntegrin alpha E is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Integrin alpha 5/CD49e
Phe45-Asn (Gln68Glu)
Accession # P11688
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse Integrin  alpha 5/CD49e
Flow Cytometry
0.25 µg/106 cells
B16‑F1 mouse melanoma cell line
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin alpha 5/CD49e

The alpha 5 subunit, also known as CD49e and VLA-5 alpha subunit, forms a heterodimer with the Integrin beta 1 subunit (CD29). Integrin alpha 5 beta 1 is a receptor for fibronectin and neural adhesion molecule L1.

Entrez Gene IDs
3678 (Human); 16402 (Mouse); 102114969 (Cynomolgus Monkey)
Alternate Names
CD49 antigen-like family member E; CD49e antigen; CD49e; Fibronectin receptor subunit alpha; fibronectin receptor, alpha subunit; FNRAVLA5A; Integrin alpha 5; integrin alpha-5; Integrin alpha-F; integrin, alpha 5 (fibronectin receptor, alpha polypeptide); ITGA5; very late activation protein 5, alpha subunit; VLA-5 alpha; VLA-5

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Citations for Mouse Integrin  alpha 5/CD49e Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Direct regulation of osteocytic connexin 43 hemichannels through AKT kinase activated by mechanical stimulation.
    Authors: Batra N, Riquelme M, Burra S, Kar R, Gu S, Jiang J
    J Biol Chem, 2014-02-22;289(15):10582-91.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation, Western Blot
  2. Mechanical stress-activated integrin alpha5beta1 induces opening of connexin 43 hemichannels.
    Authors: Batra N, Burra S, Siller-Jackson AJ, Gu S, Xia X, Weber GF, DeSimone D, Bonewald LF, Lafer EM, Sprague E, Schwartz MA, Jiang JX
    Proc. Natl. Acad. Sci. U.S.A., 2012-02-13;109(9):3359-64.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC

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