Mouse Integrin beta 3/CD61 Antibody

Name: Mouse Integrin beta 3/CD61 Antibody
Brand: R&D Systems
SKU: MAB41182
Price: 459 USD
Availability: InStock

R&D Systems | Catalog # MAB41182

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Product Specifications
Scientific Data
Applications
Flow Cytometry
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Flow Cytometry, CyTOF-ready

Cited:

Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG₁ Clone # 909114

View all Integrin beta 3/CD61 Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant mouse Integrin alpha 2b and Integrin beta 3 linked heterodimer
Leu32-Arg988 (Integrin alpha 2b) & Glu26-Asp717 (Integrin beta 3)
Accession # NP_034705 (Integrin alpha 2b) & O54890 (Integrin beta 3)

Specificity

Detects mouse Integrin beta 3/CD61 in direct ELISA.

Clonality

Monoclonal

Host

Rat

Isotype

IgG₁

Scientific Data Images for Mouse Integrin beta 3/CD61 Antibody

Detection of Integrin beta 3/CD61 in P815 Mouse Cell Line by Flow Cytometry.

P815 mouse mastocytoma cell line was stained with Rat Anti-Mouse Integrin beta 3/CD61 Monoclonal Antibody (Catalog # MAB41182, filled histogram) or isotype control antibody (Catalog # MAB005, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113).

Applications for Mouse Integrin beta 3/CD61 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/mL
Sample: P815 mouse mastocytoma cell line

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Advanced Features

Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
Spillover Popups - Visualize the spectra of individual fluorochromes
Antigen Density Selector - Match fluorochrome brightness with antigen density

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Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: Integrin beta 3/CD61

Integrin beta 3 (ITGB3) is also known as platelet glycoprotein IIIa (GPIIIa) and CD61. Integrins are integral cell-surface proteins known to participate in both cell adhesion and cell-surface mediated signaling. They are composed of an alpha chain and a beta chain. ITGB3 is an integrin beta chain that forms a heterodimer with alpha-IIb or alpha-V chains. It is involved in platelet aggregation, and serves as an anchor for fibrinogen, allowing platelets to form aggregates or clots. A functional absense of ITGB3 leads to Glanzmann's thrombasthenia, a condition where platelets are activated, but fail to form an aggregate. Alternatively spliced transcripts encoding different proteins have been described, in human.