Detects mouse Intelectin-2 in direct ELISAs and Western blots.
In direct ELISAs and Western blots, 100% cross-reactivity
with recombinant mouse (rm) Intelectin-1 is observed. In direct ELISAs, approximately 25%
cross-reactivity with recombinant human (rh) Intelectin-2 is observed, and
approximately 15% cross-reactivity with rhIntelectin-1 is observed.
Monoclonal Rat IgG1 Clone # 747421
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant mouse Intelectin-2 Ala20-Ser298 Accession # Q80ZA0
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Mouse and Rat Intelectin‑2 by Western Blot. Western blot shows lysates of mouse small intestine tissue and rat small intestine tissue. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse Intelectin‑2 Monoclonal Antibody (Catalog # MAB5358) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Intelectin‑2 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Intelectin‑2 in Mouse Intestine. Intelectin‑2 was detected in perfusion fixed frozen sections of mouse intestine using Rat Anti-Mouse Intelectin‑2 Monoclonal Antibody (Catalog # MAB5358) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to Paneth cells in intestinal glands. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
(endothelial lectin 2; also ITLN-2 and HL-2) is a 40 kDa, likely
Ca-dependent carbohydrate-binding lectin that is a member of the
X-lectin family of molecules. It is secreted by intestinal Paneth cells
and appears to participate in fungal microbe recognition. The Intelectin-2
precursor is 325 amino acids (aa) in length. It contains a 26 aa signal
sequence and a 299 aa mature region. Although mouse Intelectin-2 is reportedly
GPI-linked and mouse and human Intelectin-2 show considerable C‑terminal aa
identity, it is not known if human Intelectin-2 is also GPI-linked. There is
one fibrinogen-related domain in the mature molecule (aa 45‑210). One
potential splice variant is reported that shows a premature truncation
after Gly264. Over aa 20‑298, mouse Intelectin-2 shows 78% aa identity to human Intelectin-2.
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