Key Product Details

Species Reactivity

Mouse

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG1 Clone # 747421
View all Intelectin-2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse Intelectin-2
Ala20-Ser298
Accession # Q80ZA0

Specificity

Detects mouse Intelectin-2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, 100% cross-reactivity with recombinant mouse (rm) Intelectin-1 is observed. In direct ELISAs, approximately 25% cross-reactivity with recombinant human (rh) Intelectin-2 is observed, and approximately 15% cross-reactivity with rhIntelectin-1 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG1

Scientific Data Images for Mouse Intelectin‑2 Antibody

Detection of Mouse and Rat Intelectin-2 antibody by Western Blot.

Detection of Mouse and Rat Intelectin‑2 by Western Blot.

Western blot shows lysates of mouse small intestine tissue and rat small intestine tissue. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse Intelectin-2 Monoclonal Antibody (Catalog # MAB5358) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Intelectin-2 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Intelectin-2 antibody in Mouse Intestine by Immunohistochemistry (IHC-Fr).

Intelectin‑2 in Mouse Intestine.

Intelectin-2 was detected in perfusion fixed frozen sections of mouse intestine using Rat Anti-Mouse Intelectin-2 Monoclonal Antibody (Catalog # MAB5358) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counter-stained with hematoxylin (blue). Specific staining was localized to Paneth cells in intestinal glands. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Applications for Mouse Intelectin‑2 Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Perfusion fixed frozen sections of mouse intestine

Western Blot

2 µg/mL
Sample: Mouse small intestine tissue and rat small intestine tissue

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Intelectin-2

Intelectin-2 (endothelial lectin 2; also ITLN-2 and HL-2) is a 40 kDa, likely Ca-dependent carbohydrate-binding lectin that is a member of the X-lectin family of molecules. It is secreted by intestinal Paneth cells and appears to participate in fungal microbe recognition. The Intelectin-2 precursor is 325 amino acids (aa) in length. It contains a 26 aa signal sequence and a 299 aa mature region. Although mouse Intelectin-2 is reportedly GPI-linked and mouse and human Intelectin-2 show considerable C‑terminal aa identity, it is not known if human Intelectin-2 is also GPI-linked. There is one fibrinogen-related domain in the mature molecule (aa 45‑210). One potential splice variant is reported that shows a premature truncation after Gly264. Over aa 20‑298, mouse Intelectin-2 shows 78% aa identity to human Intelectin-2.

Alternate Names

HL-2, Intelectin-b, Intelectin2, Itln1b, ITLN2, Itlnb

Entrez Gene IDs

142683 (Human); 493583 (Mouse)

Gene Symbol

ITLN2

UniProt

Q80ZA0

Additional Intelectin-2 Products

Product Documents for Mouse Intelectin‑2 Antibody

Certificate of Analysis

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Product Specific Notices for Mouse Intelectin‑2 Antibody

For research use only

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Protocols

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