Mouse Intelectin-2 Antibody

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Detection of Mouse and Rat Intelectin‑2 by Western Blot.
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Product Details
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Mouse Intelectin-2 Antibody Summary

Species Reactivity
Detects mouse Intelectin-2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, 100% cross-reactivity with recombinant mouse (rm) Intelectin-1 is observed. In direct ELISAs, approximately 25% cross-reactivity with recombinant human (rh) Intelectin-2 is observed, and approximately 15% cross-reactivity with rhIntelectin-1 is observed.
Monoclonal Rat IgG1 Clone # 747421
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant mouse Intelectin-2
Accession # Q80ZA0
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
2 µg/mL
See below
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse and Rat Intelectin-2 antibody by Western Blot. View Larger

Detection of Mouse and Rat Intelectin‑2 by Western Blot. Western blot shows lysates of mouse small intestine tissue and rat small intestine tissue. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse Intelectin-2 Monoclonal Antibody (Catalog # MAB5358) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Intelectin-2 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry Intelectin-2 antibody in Mouse Intestine by Immunohistochemistry (IHC-Fr). View Larger

Intelectin‑2 in Mouse Intestine. Intelectin-2 was detected in perfusion fixed frozen sections of mouse intestine using Rat Anti-Mouse Intelectin-2 Monoclonal Antibody (Catalog # MAB5358) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counter-stained with hematoxylin (blue). Specific staining was localized to Paneth cells in intestinal glands. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Intelectin-2

Intelectin-2 (endothelial lectin 2; also ITLN-2 and HL-2) is a 40 kDa, likely Ca-dependent carbohydrate-binding lectin that is a member of the X-lectin family of molecules. It is secreted by intestinal Paneth cells and appears to participate in fungal microbe recognition. The Intelectin-2 precursor is 325 amino acids (aa) in length. It contains a 26 aa signal sequence and a 299 aa mature region. Although mouse Intelectin-2 is reportedly GPI-linked and mouse and human Intelectin-2 show considerable C‑terminal aa identity, it is not known if human Intelectin-2 is also GPI-linked. There is one fibrinogen-related domain in the mature molecule (aa 45‑210). One potential splice variant is reported that shows a premature truncation after Gly264. Over aa 20‑298, mouse Intelectin-2 shows 78% aa identity to human Intelectin-2.

Entrez Gene IDs
142683 (Human); 493583 (Mouse)
Alternate Names
HL-2; intelectin 2; Intelectin2; Intelectin-2; Intelectin-b; Itln1b; ITLN2; Itlnb; UNQ2789/PRO7179

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