Mouse Jagged 2 Antibody

Catalog # Availability Size / Price Qty
MAB4748
MAB4748-SP
Detection of Jagged 2 in D3 Mouse Cell Line by Flow Cytometry.
1 Image
Product Details
FAQs
Supplemental Products
Reviews

Mouse Jagged 2 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Jagged 2 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) Jagged 1, rhJagged 2, recombinant rat Jagged 1 is observed.
Source
Monoclonal Rat IgG2A Clone # 746513
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Jagged 2
Met27-Leu1084
Accession # Q9QYE5
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Flow Cytometry
2.5 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Jagged 2 antibody in D3 Mouse Cell Line antibody by Flow Cytometry. View Larger

Detection of Jagged 2 in D3 Mouse Cell Line by Flow Cytometry. D3 mouse embryonic stem cell line was stained with Rat Anti-Mouse Jagged 2 Monoclonal Antibody (Catalog # MAB4748, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Jagged 2

Mouse Jagged 2 is a 150 kDa (1) member of the Delta-Serrate-Lag-2 (DSL) family of ligands that activate LIN12/Notch proteins, and thereby regulate cell fate determinations during development (2-6). It is a type 1 transmembrane protein that is synthesized as a 1247 amino acid (aa) precursor. It contains a 23 aa signal sequence, a 1059 aa extracellular region, a 21 aa transmembrane region, and a 144 aa cytoplasmic region. The extracellular region contains four potential N-linked glycosylation sites, a DSL domain, 16 EGF-like repeats, many of which are also sites of calcium binding, and a cysteine-rich region just proximal to the transmembrane segment (3). Mouse Jagged 2 shares 93% and 90% aa sequence identity with rat and human Jagged 2, respectively. Jagged 2 is expressed highest in fetal thymus, epidermis, foregut, dorsal root ganglia, and inner ear (3). In 2-week old mice, the Jagged 2 transcript is most abundant in heart, lung, thymus, skeletal muscle, brain, and testis (3). Functionally, it is suggested that Jagged 2 engages the Notch1 pathway of signal transduction (3). It is involved in the development of the mammalian limb, branchial arches, central and peripheral nervous systems,  gamma δ T cell lineage differentiation, and the establishment of functional natural killer cell lines (4 - 6).

References
  1. Tsai, S. et al. (2000) Blood, 96:950.
  2. Shawber, C. et al. (1996) Dev. Biol. 180:370.
  3. Luo, B. et al. (1997) Mol. Cell. Biol. 17:6057.
  4. Valsecchi, V. et al. (1997) Mech. Dev. 69:203.
  5. DeHart, S. et al. (2005) Blood 105:3521.
  6. de La Coste, A. and A.A. Freitas (2006) Immunol. Lett. 102:1.
Entrez Gene IDs
3714 (Human); 16450 (Mouse); 29147 (Rat)
Alternate Names
HJ2; JAG2; Jagged 2; Jagged2; protein jagged-2; SER2

Product Datasheets

You must select a language.

x

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reviews for Mouse Jagged 2 Antibody

There are currently no reviews for this product. Be the first to review Mouse Jagged 2 Antibody and earn rewards!

Have you used Mouse Jagged 2 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review