Mouse Kallikrein 5 Antibody

Catalog # Availability Size / Price Qty
Detection of Mouse Kallikrein 5 by Western Blot.
2 Images
Product Details
Citations (1)
Supplemental Products

Mouse Kallikrein 5 Antibody Summary

Species Reactivity
Detects mouse Kallikrein 5 in ELISA.
Monoclonal Rat IgG2B Clone # 880036
Protein A or G purified from hybridoma culture supernatant
Mouse myeloma cell line NS0-derived recombinant mouse Kallikrein 5
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
2 µg/mL
See below
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse Kallikrein 5 antibody by Western Blot. View Larger

Detection of Mouse Kallikrein 5 by Western Blot. Western blot shows lysates of NMuMG mouse mammary gland epithelial cell line. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse Kallikrein 5 Monoclonal Antibody (Catalog # MAB7236) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Kallikrein 5 at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry Kallikrein 5 antibody in Hair Follicle of Mouse Skin by Immunohistochemistry (IHC-Fr). View Larger

Kallikrein 5 in Hair Follicle of Mouse Skin. Kallikrein 5 was detected in immersion fixed frozen sections of mouse skin using Rat Anti-Mouse Kallikrein 5 Monoclonal Antibody (Catalog # MAB7236) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to the inner sheath cuticle of hair follicles. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Kallikrein 5

Tissue Kallikrein 5 (KLK5) is a secreted serine protease with trypsin‑like substrate specificity. KLK5 is highly expressed in the skin, and is also known as stratum corneum tryptic enzyme due to its high expression level in this tissue. KLK5 is thought to play an important role in the process of desquamation (1). KLK5 is a potential biomarker for ovarian and breast cancers (2). It may also be a physiological activator of KLK7 in the stratum corneum (3). Recombinant mouse KLK5 is purified as the proform, and can undergo autoactivation under the appropriate conditions. KLK5 enzymatic activity can be inhibited by the following recombinant human serpins: Serpin‑A1, ‑A5, ‑F2, and ‑G1 (Catalog # 1268-PI, 1266-PI, 1470-PI, and 2488-PI).

  1. Brattsand M. and T. Egelrud (1999) J. Biol. Chem. 274:30033.
  2. Yousef G.M. et al. (2003) Cancer Res. 63:3958.
  3. Kim H. et al. (2001) Br. J. Cancer 84:543.
Entrez Gene IDs
25818 (Human); 68668 (Mouse); 690603 (Rat)
Alternate Names
EC 3.4.21; EC; Kallikrein 5; Kallikrein-like protein 2; kallikrein-related peptidase 5; KLK5; KLKL2; KLK-L2; KLK-L2EC 3.4.21.-; SCTE; SCTEkallikrein-5; Stratum corneum tryptic enzyme

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Citation for Mouse Kallikrein 5 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Keratinocytes coordinate inflammatory responses and regulate development of secondary lymphedema
    Authors: HJ Park, RP Kataru, J Shin, GD Garc A Nor, EM Encarnacio, MG Klang, E Riedel, M Coriddi, JH Dayan, BJ Mehrara
    bioRxiv : the preprint server for biology, 2023-01-21;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot


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