Mouse Kallikrein 5 Antibody (880036)

Detection of Mouse Kallikrein 5 by Western Blot.

Western blot shows lysates of NMuMG mouse mammary gland epithelial cell line. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse Kallikrein 5 Monoclonal Antibody (Catalog # MAB7236) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Kallikrein 5 at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Kallikrein 5 in Hair Follicle of Mouse Skin.

Kallikrein 5 was detected in immersion fixed frozen sections of mouse skin using Rat Anti-Mouse Kallikrein 5 Monoclonal Antibody (Catalog # MAB7236) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to the inner sheath cuticle of hair follicles. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Detection of aitzzckyosadfm9rkedu.jpg by Immunohistochemistry

Keratinocyte expression of Th2‐inducing cytokines occurs rapidly after lymphatic injury. (A) Representative H&E (top) and immunofluorescent staining for LYVE1 and CD3e (bottom), and quantification (right) of control (skin incision) tail skin and tail skin harvested 2 or 6 week after skin and lymphatic excision. Scale bar: 100 µm. Each circle represents the average of three HPF views for each mouse (n = 5–8). *p < .05, ****p < .0001. p Values were calculated by one‐way ANOVA. (B) Representative immunofluorescent images (left) and quantification (right) of KRT14, KLK5, PAR2, TSLP, IL‐33, Ki67 and IL1 alpha staining in tail skin harvested 2 week after surgery from control and lymphedema (LE) mice. Scale bar: 100 µm. Each circle represents the average of three HPF views for each mouse (n = 5–6). *p < .05, ****p < .0001. p Values were calculated by unpaired Student's t‐test. (C) Relative mRNA expression by qPCR tail skin harvested 2 week after surgery from control and LE mice (n = 5). mRNA expression was normalised to beta ‐actin expression. Each box represents one mouse. *p < .05, **p < .01, ***p < .001. p Values were calculated by Mann–Whitney test. Fold changes from control are shown in parentheses. (D) Representative western blot (left) and quantification (right; relative to beta ‐actin) of KLK5 and PAR2 in tail skin harvested 2 week after surgery from control and LE mice (left). Each circle represents each mouse (n = 5–6). *p < .05, **p < .01. p Values were calculated by Mann–Whitney test. (E) TSLP and IL‐33 ELISA from protein lysates of control and LE tail skin (n = 5–7). Each circle represents one mouse. p Values were calculated by Mann–Whitney test. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/42226615), licensed under a CC-BY license. Not internally tested by R&D Systems.