MKI67 (also Ki67 and TSG126) is a 350-370 kDa nuclear protein that belongs to a molecular group comprised of mitotic chromosome-associated proteins. Ki67 was originally recognized as an antigen associated with the monoclonal Ki67 antibody raised against Hodgkin's lymphoma nuclear material. Ki67 is contextually expressed, being potentially found in all cells that are not in the Go phase of the cell cycle. Thus, MKI67 qualifies as a cell proliferation marker. Functionally, Ki67 is known to interact with 160 kDa Hklp2, a protein that promotes centrosome separation and spindle bipolarity. It also directly interacts with NIFK, and apparently binds to UBF, thus playing a role in rRNA synthesis. Mouse MKI67 is 3177 amino acids (aa) in length. It contains one FHA domain (aa 8-101), followed by sixteen 120 aa repeats (aa 993-2872). There are two potential isoform variants. One isoform shows a 19 aa substitution for 1120-3177, while a second isoform contains a deletion of aa 1169-1409. Over aa 3053-3177, mouse Ki67 shares 46% and 74% aa sequence identity with the human and rat orthologs to Ki67, respectively.
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Human, Mouse, Transgenic Mouse
Applications
Validated:
Immunocytochemistry
Cited:
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
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Product Specifications
Immunogen
E. coli-derived recombinant mouse Ki67/MKI67
Asn3053-Ser3177
Accession # NP_001074586
Asn3053-Ser3177
Accession # NP_001074586
Specificity
Detects mouse Ki67/MKI67 in direct ELISAs. In direct ELISAs, less than 5% cross-reactivity with recombinant human Ki67/MKI67 is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Mouse Ki67/MKI67 Antibody
Detection of Ki67/MKI67 in RAW264.7cells and Neuro2A cells.
Ki67/MKI67 was detected in immersion fixed RAW 264.7 mouse monocyte/macrophage cell line (Positive) and Neuro‑2A mouse neuroblastoma cell line (Positive) using Sheep Anti-Mouse Ki67/MKI67 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7649) at 1.7 µg/ml for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to the nucleus. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Ki67/MKI67 in NIH‑3T3 Mouse Cell Line.
Ki67/MKI67 was detected in immersion fixed NIH-3T3 mouse embryonic fibroblast cell line using Sheep Anti-Mouse Ki67/MKI67 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7649) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel; Catalog # NL010) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei and nucleoli. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Mouse Ki67/MKI67 Antibody
Application
Recommended Usage
Immunocytochemistry
1-15 µg/mL
Sample: Immersion fixed NIH‑3T3 mouse embryonic fibroblast cell line, RAW264.7 mouse monocyte/macrophage cell line, and Neuro-2A mouse neurosblastoma cell line
Sample: Immersion fixed NIH‑3T3 mouse embryonic fibroblast cell line, RAW264.7 mouse monocyte/macrophage cell line, and Neuro-2A mouse neurosblastoma cell line
Reviewed Applications
Read 2 reviews rated 5 using AF7649 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.<br/>*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: Ki67/MKI67
Long Name
Antigen Identified by Monoclonal Antibody Ki67
Alternate Names
Ki-67, KIA, MIB-1, MKI67, PPP1R105, TSG126
Gene Symbol
MKI67
UniProt
Additional Ki67/MKI67 Products
Product Documents for Mouse Ki67/MKI67 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Mouse Ki67/MKI67 Antibody
For research use only
Related Research Areas
Citations for Mouse Ki67/MKI67 Antibody
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Customer Reviews for Mouse Ki67/MKI67 Antibody (2)
5 out of 5
2 Customer Ratings
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Application: ImmunohistochemistrySample Tested: Ovary tissueSpecies: MouseVerified Customer | Posted 12/01/2024Ki67 expressed in cyan color in ovary. IHC-P.
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Application: IF/ICCSample Tested: Mouse aortic rootSpecies: MouseVerified Customer | Posted 01/23/2018immunofluorescence staining of the aortic root forproliferation marker Ki67 (green), DAPI (blue)
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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