Mouse Kininogen Light Chain Antibody

Catalog # Availability Size / Price Qty
MAB22061
MAB22061-SP
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Mouse Kininogen Light Chain Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Kininogen Light Chain in direct ELISAs and Western blots. In Western blots, reacts with full length Kininogen (aa 21‑661) and several forms of the light chain starting at residue 389, and does not react with the heavy chain. In Western blots, no cross-reactivity with recombinant mouse (rm) Cystatin A, B, C, E/M, recombinant human (rh) Cystatin D, F, S, SA, SN, rmCystatin/Stefin Homolog, rmFetuin A, rhFetuin B, rmHPRG, rhKininogen, or rhKininostatin is observed.
Source
Monoclonal Rat IgG2A Clone # 288424
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Kininogen
Glu21-Ser661
Accession # O08677
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse Kininogen High Molecular Weight (HKa) (Catalog # 2206-PI)
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Mouse Kininogen High Molecular Weight (HKa) (Catalog # 2206-PI), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Kininogen

Kininogen, also known as alpha 2-thiol proteinase inhibitor, is a multi-function protein. There are two alternatively spliced forms, designated as the high molecular weight (HMW) and low MW (LMW) forms (1). The mouse HMW form is synthesized as a 661 amino acid (aa) precursor with a signal peptide (aa 1‑20). The mature chain (aa 21‑661) is further processed into the heavy (aa 21‑379) and the light (aa 389‑661) chains. The active peptide bradykinin (aa 380‑388) is released which has a variety of functions including muscle contraction, hypotension and inflammation. The heavy chain consists of three cystatin-like domains which are responsible for inhibiting cysteine proteases. The light chain consists of a His‑rich domain which is associated with the clotting activity. In comparison to the HMW form, the LMW Kininogen (432 residues) has the same sequence in its heavy chain and bradykinin but has a different sequence in its light chain (aa 401‑432). The LMW form is not involved in blood clotting.

References
  1. Takano, M. et al. (1997) Biochim. Biophys. Acta 1352:222.
Entrez Gene IDs
3827 (Human); 16644 (Mouse); 24903 (Rat)
Alternate Names
alpha 2-thiol Proteinase Inhibitor; BDK; Kininogen; KNG1

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