Mouse Klotho Biotinylated Antibody
Mouse Klotho Biotinylated Antibody Summary
Accession # BAA25307
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Klotho, also called Klotho-alpha, is the founding member of the Klotho family within the glycosidase-1 superfamily (1, 2). Klotho is expressed in areas concerned with calcium regulation, predominantly in the kidney distal convoluted tubules, but also in the brain choroid plexus (which produces cerebrospinal fluid) and the parathyroid (1). The 1014 amino acid (aa) type I transmembrane protein contains a 34 aa signal sequence, a 948 aa extracellular domain (ECD) containing two extracellular glycosidase-like domains, a 21 aa transmembrane domain and an 11 aa intracellular domain. Within the ECD, mouse Klotho shares 95%, 87% and 87% aa identity with rat, human and equine Klotho, respectively. Although a truncated 554 aa isoform predicts a soluble 70 kDa form, the soluble form found in plasma and cerebrospinal fluid is a 130 kDa form produced by proteolytic cleavage of the glycosylated 135 kDa full-length Klotho (3, 4). A prominent intracellular 120 kDa form of Klotho is localized to endoplasmic reticulum and Golgi membranes (4). Klotho is named for the Greek goddess who spins the thread of life. The phenotype of Klotho-deficient mice resembles premature aging, including arteriosclerosis, osteoporosis, skin atrophy, infertility, emphysema and premature death (2). Conversely, excess Klotho extends lifespan (5). Klotho acts as a cofactor for interaction of FGF23 with FGF R1 (6). This interaction negatively regulates 1 alpha -hydroxylase, the rate-limiting enzyme in the synthesis of 1,25(OH)2D3 (vitamin D) (7). Klotho-deficient mice show severe hyperphosphatemia and ectopic calcification of soft tissues due to excess vitamin D (2, 7). Both Klotho and Klotho-beta are cofactors for FGF19 binding (8). Klotho also shows glucuronidase activity which activates the renal ion channel TRPV5 to reabsorb urinary calcium (9). Klotho has been reported to downregulate insulin or IGF-1 signaling in adipocytes, to bind and antagonize Wnt molecules, and to facilitate release of parathyroid hormone (10 - 12).
- Nabeshima, Y. (2006) Sci. Aging Knowl. Environ. 8:pe11.
- Kuro-o, M. et al. (1997) Nature 390:45.
- Shiraki-Iida, T. et al. (1998) FEBS Lett. 424:6.
- Imura, A. et al. (2004) FEBS Lett. 565:143.
- Kurosu, H. et al. (2005) Science 309:1829.
- Kurosu, H. et al. (2006) J. Biol. Chem. 281:6120.
- Tsujikawa, H. et al. (2003) Mol. Endocrinol. 17:2393.
- Wu, X. et al. (2007) J. Biol. Chem. 282:29069.
- Chang, Q. et al. (2005) Science 310:490.
- Yamamoto, M. et al. (2005) J. Biol. Chem. 280:38029.
- Liu, H. et al. (2007) Science 317:803.
- Imura, A. et al. (2007) Science 316:1615.
Citations for Mouse Klotho Biotinylated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 2
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The Klotho proteins in health and disease
Authors: M Kuro-O
Nat Rev Nephrol, 2019-01-01;0(0):.
Sample Types: Whole Tissue
Circulating alphaKlotho influences phosphate handling by controlling FGF23 production.
Authors: Smith R
J. Clin. Invest., 2012-11-26;122(12):4710-5.
Sample Types: Plasma
Applications: ELISA Development
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