Mouse KLRG1 Antibody

Recombinant Monoclonal Antibody.
  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse KLRG1 in flow cytometry.
  • Source
    Recombinant Monoclonal Rabbit IgG Clone # 1151A
  • Purification
    Protein A or G purified from cell culture supernatant
  • Immunogen
    Chinese hamster ovary cell line CHO-derived mouse KLRG1
    Glu57-Tyr188
    Accession # O88713
  • Formulation
    Supplied as a solution in PBS containing BSA, Glycerol and Sodium Azide. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    0.5 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of KLRG1 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse CD161/NK1.1 APC‑conjugated Monoclonal Antibody (Catalog # FAB7614A) and either (A) Rabbit Anti-Mouse KLRG1 Monoclonal Antibody (Catalog # MAB6944) or (B) Normal Rabbit IgG Control
(Catalog #AB-105-C) followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0110).
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    • 12 months from date of receipt, -20 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after opening.
    • 6 months, -20 °C under sterile conditions after opening.
Background: KLRG1

KLRG1 (Killer cell Lectin‑like Receptor G1), also called MAFA (Mast cell Function Associated), is an inhibitory type II transmembrane glycoprotein of the C‑type lectin family, designated CLEC15A (1). Mature mouse KLRG1 consists of a 33 amino acid (aa) cytoplasmic domain with one Immunoreceptor Tyrosine‑based Inhibitory Motif (ITIM), a 23 aa transmembrane segment, and a 132 aa extracellular domain (ECD) with one C‑type lectin domain (CTLD) (2). Within the ECD, mouse KLRG1 shares 57% and 80% aa sequence identity with human and rat KLRG1, respectively. Alternate splicing generates additional isoforms of mouse KLRG1 that lack either the CTLD or the CTLD, transmembrane segment, and a portion of the cytoplasmic domain (3). KLRG1 is expressed as a 30 ‑ 40 kDa N‑glycosylated molecule that forms disulfide‑linked homodimers, trimers, and tetramers (4, 5). It is expressed on subpopulations of CD8+, CD4+, regulatory, and gamma/delta T cells as well as on NK cells (2, 4, 6 ‑ 8). KLRG1 is expressed on T cells found in cord blood, but it is down‑regulated postnatally and is subsequently re‑expressed on antigen‑exposed T cells (7, 9). It is expressed by a greater proportion of CD8+ T cells in the elderly and by virus‑specific CD8+ T cells during chronic virus infection (10 ‑ 12). KLRG1 binds to E-, N-, and R-Cadherins, triggering ITIM‑dependent KLRG1 signaling and inhibition of T cell activation (5, 13, 14). The response is bi‑directional, as KLRG1 binding to E‑Cadherin on dendritic cells (DC) can induce an anti‑inflammatory DC phenotype (increased IL‑10 production and decreased IL‑6 and TNF‑ alpha production) (15).

  • References:
    1. Henson, S.M. and A.N. Akbar (2009) Age 31:285.
    2. Hanke, T. et al. (1998) Eur. J. Immunol. 28:4409.
    3. Voehringer, D. et al. (2001) Immunogenetics 52:206.
    4. Corral, L. et al. (2000) Eur. J. Immunol. 30:920.
    5. Rosshart, S. et al. (2008) Eur. J. Immunol. 38:3354.
    6. Voehringer, D. et al. (2002) Blood 100:3698.
    7. Beyersdorf, N. et al. (2007) Eur. J. Immunol. 37:3445.
    8. Eberl, M. et al. (2005) J. Leukoc. Biol. 77:67.
    9. Marcolino, I. et al. (2004) Eur. J. Immunol. 34:2672.
    10. Ouyang, Q. et al. (2003) Exp. Gerontol. 38:911.
    11. Thimme, R. et al. (2005) J. Virol. 79:12112.
    12. Cush, S.S. and E. Flano (2011) J. Immunol. 186:4051.
    13. Ito, M. et al. (2006) J. Exp. Med. 203:289.
    14. Tessmer, M.S. et al. (2007) Int. Immunol. 19:391.
    15. Banh, C. et al. (2009) Blood 114:5299.
  • Long Name:
    Killer Cell Lectin-like Receptor Subfamily G Member 1
  • Entrez Gene IDs:
    10219 (Human); 50928 (Mouse); 58975 (Rat)
  • Alternate Names:
    2F1; CLEC15A; CLEC15AMAFA-LIKE; C-type lectin domain family 15 member A; C-type lectin domain family 15, member A; ITIM-containing receptor MAFA-L; killer cell lectin-like receptor subfamily G member 1; killer cell lectin-like receptor subfamily G, member 1; KLRG1; MAFA; MAFAL; MAFA-L; MAFA-like receptor; MAFAMAFA-2F1; mast cell function-associated antigen (ITIM-containing); Mast cell function-associated antigen; MGC13600
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Normal Rabbit IgG Control

Ctrl AB-105-C 42  
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Normal Rabbit IgG Control

Ctrl, CyTOF-ready MAB1050  
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Secondary Antibodies
Description Application Cat# Citations Images  

Rabbit IgG HRP-conjugated Antibody

WB HAF008 23  
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Donkey Anti-Rabbit IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL004 10
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Donkey Anti-Rabbit IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL005 2
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Rabbit IgG APC-conjugated Antibody

Flow F0111 1  
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Rabbit IgG PE-conjugated Antibody

Flow F0110 2  
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Donkey Anti-Rabbit IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL006 4
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Rabbit IgG Fluorescein-conjugated Antibody

Flow F0112 5  
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Goat Anti-Rabbit IgG Biotinylated Antibody

WB BAF008 4
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC003  
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Rabbit IgG Antibody

WB AF008 1
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Donkey Anti-Rabbit IgG (H+L) Antibody

D-301-C-ABS2
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