Detection of Mouse Legumain/Asparaginyl Endopeptidase by Western Blot. Western blot shows lysates of J774A.1 mouse reticulum cell sarcoma macrophage cell line and Neuro‑2A mouse neuroblastoma cell line. PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Mouse Legumain/Asparaginyl Endopeptidase Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2058) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for Legumain/Asparaginyl Endopeptidase at approximately 37 and 56 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Legumain/Asparaginyl Endopeptidase
Legumain is a lysosomal cysteine protease found in all mouse tissues examined, but was particularly abundant in kidney and placenta (1). Legumain plays a pivotal role in the endosomal/lysosomal degradation system because the Legumain deficiency causes the accumulation of pro cathepsins B, H and L, another group of lysosomal cysteine proteases (2). Over-expression of Legumain in tumors is significant for invasion/metastasis (3). Also known as asparaginyl endopeptidase, it specifically cleaves peptide bonds with Asn at the P1 position. Nevertheless, it also cleaves peptide bonds with Asp at the P1 position. Auto-activation of pro Legumain involves both types of cleavage, which results in the removal of the pro peptides in both C- and N-termini (4). In addition, Legumain activates pro MMP-2 and processes bacterial antigens for MHC class II presentation and pro thymosin alpha to thymosin alpha 1 and thymosin alpha 11, two acidic peptides with immunoregulatory properties (5-7).
Chen, J.M. et al. (1998) Biochem. J. 335:111.
Shirahama-Noda, K. et al. (2003) J. Biol. Chem. 278:33194.
Liu, C. et al. (2003) Cancer Res. 63: 2957.
Li D.N. et al. (2003) J. Biol. Chem. 278:38980.
Chen, J.M. et al. (2001) Biol. Chem. 382:777.
Schwarz, G. et al. (2002) Biol. Chem. 383:1813.
Sarndeses, C.S. et al. (2003) J. Biol. Chem. 278:13286.
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