Mouse Lgr5/GPR49 APC-conjugated Antibody
Mouse Lgr5/GPR49 APC-conjugated Antibody Summary
Accession # Q9Z1P4
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Lgr5/GPR49 in HEK293 Human Cell Line Transfected with Mouse Lgr5/GPR49 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) mouse Lgr5/GPR49 or (B) irrelevent transfectants and eGFP was stained with Rat Anti-Mouse Lgr5/GPR49 APC-conjugated Monoclonal Antibody (Catalog # FAB82401A). Quadrant markers were set based on control antibody staining (Catalog # IC013A). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Leucine‑rich repeat G‑protein‑coupled Receptor 5 (Lgr5), also known as GPR49, is a 907 amino acid (aa), approximately 97 kDa (calculated), seven‑transmembrane glycoprotein receptor in the Lgr family of cell surface receptors. The subfamily of Lgrs comprising Lgr4, Lgr5, and Lgr6 are G‑protein‑independent mediators of the potentiating effect of R‑Spondins on Wnt signaling. Lgr5 binds and forms complexes with R‑Spondins, Frizzled Wnt receptors and LRP Wnt co‑receptors. The region of the mouse Lgr5 long extracellular domain used as an immunogen shares 90% and 95% amino acid sequence identity with human and rat Lgr5, respectively. Lgr5 is found on embryonic and adult epithelial stem cells. Lgr5+ stem cells can produce all epithelial cell types of the intestinal crypts. It is upregulated in stem cells that give rise to cancers such as intestinal, hepatocellular, pancreatic and ovarian carcinomas.
Citations for Mouse Lgr5/GPR49 APC-conjugated Antibody
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PD-L1 interacts with Frizzled 6 to activate beta-catenin and form a positive feedback loop to promote cancer stem cell expansion
Authors: L Fu, J Fan, S Maity, G McFadden, Y Shi, W Kong
Sample Types: Whole Cells
Applications: Flow Cytometry
Impact of a high?fat diet on intestinal stem cells and epithelial barrier function in middle?aged female mice
Authors: Y Xie, F Ding, W Di, Y Lv, F Xia, Y Sheng, J Yu, G Ding
Mol Med Rep, 2020-01-13;21(3):1133-1144.
Sample Types: Organoid
Applications: Flow Cytometry
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