Mouse Lgr5/GPR49 Antibody

Catalog # Availability Size / Price Qty
MAB82401
MAB82401-SP

Save Up to 40% on RUO Reagents with BIOSPRING24 (See Details)

Detection of Lgr5/GPR49 in HEK293 Human Cell Line Transfected with Mouse Lgr5/GPR49 and eGFP by Flow Cytometry.
1 Image
Product Details
FAQs
Supplemental Products
Reviews

Mouse Lgr5/GPR49 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Lgr5/GPR49 in direct ELISAs. Stains mouse Lgr5 transfected cells but not irrelevant transfectants in Flow Cytometry.
Source
Monoclonal Rat IgG2B Clone # 889901
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse Lgr5/GPR49 N-terminal peptide
Ala21-Cys34
Accession # Q9Z1P4
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Flow Cytometry
2.5 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Lgr5/GPR49 antibody in HEK293 Human Cell Line Transfected with Mouse Lgr5/GPR49 and eGFP antibody by Flow Cytometry. View Larger

Detection of Lgr5/GPR49 in HEK293 Human Cell Line Transfected with Mouse Lgr5/GPR49 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with (A) mouse Lgr5/GPR49 or (B) irrelevant transfectant and eGFP was stained with Rat Anti-Mouse Lgr5/GPR49 Monoclonal Antibody (Catalog # MAB82401) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). Quadrant markers were set based on control antibody staining (Catalog # MAB0061).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Loading...
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Lgr5/GPR49

Leucine‑rich repeat G‑protein‑coupled Receptor 5 (Lgr5), also known as GPR49, is a 907 amino acid (aa), approximately 97 kDa (calculated), seven‑transmembrane glycoprotein receptor in the Lgr family of cell surface receptors. The subfamily of Lgrs comprising Lgr4, Lgr5, and Lgr6 are G‑protein‑independent mediators of the potentiating effect of R‑Spondins on Wnt signaling. Lgr5 binds and forms complexes with R‑Spondins, Frizzled Wnt receptors and LRP Wnt co‑receptors. The region of the mouse Lgr5 long extracellular domain used as an immunogen shares 90% and 95% amino acid sequence identity with human and rat Lgr5, respectively. Lgr5 is found on embryonic and adult epithelial stem cells. Lgr5+ stem cells can produce all epithelial cell types of the intestinal crypts. It is upregulated in stem cells that give rise to cancers such as intestinal, hepatocellular, pancreatic and ovarian carcinomas.

Long Name
Leucine-rich Repeat Containing G Protein-coupled Receptor 5
Entrez Gene IDs
8549 (Human); 14160 (Mouse); 299802 (Rat); 102120216 (Cynomolgus Monkey)
Alternate Names
FEX; G protein-coupled receptor 49; GPR49; GPR49G-protein coupled receptor HG38; GPR67; G-protein coupled receptor 49; G-protein coupled receptor 67; GRP49; HG38; leucine-rich repeat containing G protein-coupled receptor 5; leucine-rich repeat-containing G protein-coupled receptor 5; leucine-rich repeat-containing G-protein coupled receptor 5; Lgr5; MGC117008; orphan G protein-coupled receptor HG38

Product Datasheets

You must select a language.

x

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Mouse Lgr5/GPR49 Antibody

There are currently no reviews for this product. Be the first to review Mouse Lgr5/GPR49 Antibody and earn rewards!

Have you used Mouse Lgr5/GPR49 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review