Mouse LIF R alpha Antibody MAB5990: R&D Systems

Mouse LIF R alpha Antibody

  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse LIF R alpha in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human LIF R alpha is observed.
  • Source
    Monoclonal Rat IgG1 Clone # 673602
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse LIF R alpha
    Leu44-Ser828
    Accession # P42703
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    0.25 µg/106 cells
    See below
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of LIF R alpha in D3 Mouse Cell Line by Flow Cytometry. D3 mouse embryonic stem cell line was stained with Rat Anti-Mouse LIF R alpha Monoclonal Antibody (Catalog # MAB5990, filled histogram) or isotype control antibody (Catalog # MAB005, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113).
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: LIF R alpha
Leukemia Inhibitory Factor Receptor alpha (LIF R alpha ), also known as LIFR beta and CD118, is a 190 kDa type I transmembrane protein in the Interleukin-6 receptor family. Members of this family mediate the biological effects of Cardiotrophin-1, CLC, CNTF, IL-6, IL-11, IL-27, and Oncostatin M (1). Mature mouse LIF R alpha consists of a 785 amino acid (aa) extracellular domain (ECD) with two cytokine receptor homology domains, one WSxWS motif, and three fibronectin type III repeats, followed by a 25 aa transmembrane segment and a 239 aa cytoplasmic domain (2, 3). Within the ECD, mouse LIF R alpha shares 73% and 90% aa sequence identity with human and rat LIF R alpha, respectively. Alternative splicing generates a 90 kDa soluble form of the mouse LIF R alpha ECD (4). LIF R alpha binds the pleiotropic cytokine LIF with low affinity, and the soluble isoform retains LIF-binding activity (5). Binding affinity is increased by the ligand-induced association of LIF R alpha with the signal transducing subunit gp130 (6, 7). The LIF R alpha /gp130 receptor complex also transduces Oncostatin M signals, although LIF R alpha alone does not interact with Oncostatin M (6). gp130 associates with different ligand-specific receptors to form signaling receptor complexes for the other IL-6 family ligands (1). The CNTF receptor is a ternary complex that contains CNTF R alpha and gp130 as well as LIF R alpha (8, 9). LIF R alpha is widely expressed, and LIF induces the proliferation, differentiation, and activation of cells in many tissues (10, 11). In particular, LIF R alpha plays an important role in several aspects of early pregnancy such as blastocyst implantation in the uterus (4, 12-14).
  • References:
    1. Muller-Newen, G. (2003) Science STKE 2003:pe40.
    2. Gearing, D.P. et al. (1991) EMBO J. 10:2839.
    3. Tomida, M. et al. (1994) J. Biochem. 115:557.
    4. Owczarek, C.M. et al. (1996) J. Biol. Chem. 271:5495.
    5. Layton, M.J. et al. (1992) Proc. Natl. Acad. Sci. 89:8616.
    6. Gearing, D.P. et al. (1992) Science 255:1434.
    7. Giese, B. et al. (2005) J. Cell Sci. 118:5129.
    8. Ip, N.Y. et al. (1992) Cell 69:1121.
    9. Davis, S. et al. (1993) Science 260:1805.
    10. Metcalf, D (2003) Stem Cells 21:5.
    11. Kubota, Y. et al. (2008) J. Clin. Invest. 118:2393.
    12. Paiva, P. et al. (2009) Cytokine Growth Factor Rev. 20:319.
    13. Stewart, C.L. et al. (1992) Nature 359:76.
    14. Cheng, J.-G. et al. (2001) Proc. Natl. Acad. Sci. 98:8680.
  • Long Name:
    Leukemia Inhibitory Factor Receptor alpha
  • Entrez Gene IDs:
    3977 (Human); 16880 (Mouse); 81680 (Rat)
  • Alternate Names:
    CD118 antigen; CD118; FLJ98106; FLJ99923; leukemia inhibitory factor receptor alpha; leukemia inhibitory factor receptor; LIF R alpha; LIF R beta; LIF receptor; LIFR; LIF-R; LIFRa; SJS2; STWS; SWS
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Rat IgG1 Isotype Control

Ctrl MAB005 37  
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Normal Rat IgG Control

Ctrl 6-001-A 9
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Normal Rat IgG Control (Azide Free)

Ctrl 6-001-F 1
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Rat IgG1 Isotype Control

Ctrl MAB005R
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Secondary Antibodies
Description Application Cat# Citations Images  

Rat IgG HRP-conjugated Antibody

WB, Simple Western HAF005 7  
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Goat Anti-Rat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL013 2
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Goat Anti-Rat F(ab)2 IgG (H+L)
APC-conjugated Antibody

Flow F0113 2  
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Rat F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0105B 2  
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Goat Anti-Rat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL015 1
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Goat Anti-Rat IgG Biotinylated Antibody

WB BAF005 2
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Goat Anti-Rat IgG Antibody

WB AF005 1
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Rat IgG VisUCyte HRP Polymer Antibody

IHC VC005  
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Rat F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0104B  
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Goat Anti-Rat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL014
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Goat Anti-Rat F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0115 1
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