Detection of Mouse Lipocalin-13 by Western Blot.
Western blot shows lysates of mouse liver tissue, mouse skeletal muscle tissue, mouse epididymis tissue, and mouse mammary gland tissue. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Mouse Lipocalin-13 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7974) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Lipocalin-13 at approximately 27 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
LCN-13 (Lipocalin 13; also Odorant-binding protein 2a) is a secreted, 26-28 kDa member of the LCN family, calycin superfamily of molecules. Its name is derived from Greek, and denotes the fact that lipocalins are known to be "fat-wrapping", or fat binding proteins that interact with small lipophilic molecules within a central cavity. LCN-13 is expressed by hepatocytes and smooth muscle cells. It is known to have antidiabetogenic effects, including the suppression of hepatic glucose production, the uptake of circulating glucose by adipocytes, and the induction of fatty acid oxidation by liver, possibly by stimulating CPT1 alpha production. Mouse LCN‑13 is synthesized as a 176 amino acid (aa) precursor that contains a 19 aa signal sequence and a 157 aa mature region. Although no splice forms have been reported for LCN-13 to date, a smaller, presumably proteolytically processed 23-26 kDa isoform has been noted on SDS-PAGE. Mature mouse LCN-13 shares 69% and 39% aa sequence identity with rat LCN-13 and human odorant-binding protein 2b, respectively.
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