Mouse Lipocalin-2/NGAL Antibody

Catalog # Availability Size / Price Qty
Detection of Mouse Lipocalin‑2/NGAL by Western Blot.
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Product Details
Citations (11)
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Mouse Lipocalin-2/NGAL Antibody Summary

Species Reactivity
Detects recombinant mouse (rm)Lipocalin-2 in direct ELISAs and Western blots. In these formats, does not cross-react with rhLipocalin-1 or rhLipocalin‑2.
Monoclonal Rat IgG2A Clone # 228418
Protein A or G purified from hybridoma culture supernatant
Mouse myeloma cell line NS0-derived recombinant mouse Lipocalin-2
aa 21-200
Accession # P11672
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.


Recommended Concentration
Western Blot
0.5 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse Lipocalin-2/NGAL antibody by Western Blot. View Larger

Detection of Mouse Lipocalin‑2/NGAL by Western Blot. Western blot shows lysates of mouse spleen tissue, mouse uterus tissue, and mouse epididymis tissue. PVDF membrane was probed with 0.5 µg/mL of Rat Anti-Mouse Lipocalin-2/NGAL Monoclonal Antibody (Catalog # MAB1857) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Lipocalin-2/NGAL at approximately 23 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

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Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Lipocalin-2/NGAL

Lipocalin-2, also known as Neutrophil Gelatin-Associated Lipocalin (NGAL) and Siderocalin, mediates the innate immune response to bacterial infection by sequestering iron-laden siderophores.

Long Name
Neutrophil Gelatinase-associated Lipocalin
Entrez Gene IDs
3934 (Human); 16819 (Mouse); 170496 (Rat); 102137149 (Cynomolgus Monkey)
Alternate Names
24p3; 25 kDa alpha-2-microglobulin-related subunit of MMP-9; HNL; LCN2; lipocalin 2 (oncogene 24p3); lipocalin 2; Lipocalin2; Lipocalin-2; migration-stimulating factor inhibitor; MSFI; neutrophil gelatinase-associated lipocalin; NGAL; NGALlipocalin-2; Oncogene 24p3; p25; Siderocalin; Uterocalin

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Citations for Mouse Lipocalin-2/NGAL Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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  1. Critical changes in hypothalamic gene networks in response to pancreatic cancer as found by single-cell RNA sequencing
    Authors: C Huisman, MA Norgard, PR Levasseur, SM Krasnow, MGP van der Wi, B Olson, DL Marks
    Molecular Metabolism, 2022-01-11;0(0):101441.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. Macrophage-Derived Iron-Bound Lipocalin-2 Correlates with Renal Recovery Markers Following Sepsis-Induced Kidney Damage
    Authors: C Mertens, L Kuchler, A Sola, R Guiteras, S Grein, B Brüne, A von Knethe, M Jung
    Int J Mol Sci, 2020-10-13;21(20):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: Immunoprecipitation
  3. Combination of In Situ Lcn2 pRNA-RNAi Nanotherapeutics and iNSC Transplantation Ameliorates Experimental SCI in Mice
    Authors: A Braga, S Bandiera, J Verheyen, R Hamel, C Rutigliani, F Edenhofer, JA Smith, S Pluchino
    Mol. Ther., 2020-08-05;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  4. The Fibrin Cleavage Product B?15-42 Channels Endothelial and Tubular Regeneration in the Post-acute Course During Murine Renal Ischemia Reperfusion Injury
    Authors: D Fischer, C Seifen, P Baer, M Jung, C Mertens, B Scheller, K Zacharowsk, R Hofmann, TJ Maier, A Urbschat
    Front Pharmacol, 2018-04-27;9(0):369.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  5. RNA Nanotherapeutics for the Amelioration of Astroglial Reactivity
    Authors: JA Smith, A Braga, J Verheyen, S Basilico, S Bandiera, C Alfaro-Cer, L Peruzzotti, D Shu, F Haque, P Guo, S Pluchino
    Mol Ther Nucleic Acids, 2017-11-24;10(0):103-121.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: ICC
  6. Neutrophil gelatinase?associated lipocalin as a potential novel biomarker for ventilator?associated lung injury
    Authors: R Xiao, R Chen
    Mol Med Rep, 2017-04-07;15(6):3535-3540.
    Species: Mouse
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC, Western Blot
  7. Lipocalin-2 Promotes Pancreatic Ductal Adenocarcinoma by Regulating Inflammation in the Tumor Microenvironment
    Authors: S Gomez-Chou, A Swidnicka-, N Badi, M Chavez-Tom, GB Lesinski, T Bekaii-Saa, MR Farren, TA Mace, C Schmidt, Y Liu, D Deng, RF Hwang, L Zhou, TT Moore, D Chatterjee, H Wang, X Leng, RB Arlinghaus, CD Logsdon, Z Cruz-Monse
    Cancer Res, 2017-03-01;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  8. Differential role of lipocalin 2 during immune complex-mediated acute and chronic inflammation in mice.
    Authors: Shashidharamurthy R, Machiah D, Aitken J, Putty K, Srinivasan G, Chassaing B, Parkos C, Selvaraj P, Vijay-Kumar M
    Arthritis Rheum, 2013-04-01;65(4):1064-73.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  9. Molecular MRI of murine atherosclerotic plaque targeting NGAL: a protein associated with unstable human plaque characteristics.
    Authors: te Boekhorst BC, Bovens SM, Hellings WE, van der Kraak PH, van de Kolk KW, Vink A, Moll FL, van Oosterhout MF, de Vries JP, Doevendans PA, Goumans MJ, de Kleijn DP, van Echteld CJ, Pasterkamp G, Sluijter JP
    Cardiovasc. Res., 2010-10-28;89(3):680-8.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  10. Absence of functional Hfe protects mice from invasive Salmonella enterica serovar Typhimurium infection via induction of lipocalin-2.
    Authors: Nairz M, Theurl I, Schroll A, Theurl M, Fritsche G, Lindner E, Seifert M, Crouch ML, Hantke K, Akira S, Fang FC, Weiss G
    Blood, 2009-08-21;114(17):3642-51.
    Species: Mouse
    Sample Types: Serum
    Applications: Immunoprecipitation
  11. Interferon-gamma limits the availability of iron for intramacrophage Salmonella typhimurium.
    Authors: Nairz M, Fritsche G, Brunner P, Talasz H, Hantke K, Weiss G
    Eur. J. Immunol., 2008-07-01;38(7):1923-36.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot


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