Mouse LRPAP Antibody

(1 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse LRPAP in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross‑reactivity with recombinant human LRPAP is observed.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant mouse LRPAP
    Gln29-Leu360
    Accession # P55302
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Mouse LRPAP by Western Blot. Western blot shows lysates of D3 mouse embryonic stem cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse LRPAP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4480) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for LRPAP at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: LRPAP

LRPAP (LDL receptor-related protein-associated protein 1; also named RAP) is a ubiquitously expressed 39 kDa molecular chaperone for LDL receptor family proteins (1, 2). Mature mouse LRPAP is 332 amino acids (aa) in length and secreted into the ER/Golgi of the cell. It shares 77% and 97% aa sequence identity with human and rat LRPAP, respectively. LRPAP contains three approximately 100 aa alpha -helical domains (D1‑D3). The D1 domain contains a low affinity binding site for LRP, and the associated D2 and D3 domains bind LRP with high affinity (4). Domains D2 and D3 interact with each other, while D1 is independent (3). The majority of LRPAP is localized in the endoplasmic reticulum and Golgi (5). LRPAP prevents the premature interaction of LRP, LRP2/megalin, and VLDLR with their co-expressed ligands, thereby promoting proper receptor folding and export from the ER (6‑8). Protonation of conserved histidine residues within the D3 domain induces the separation of LRPAP and LRP in the relatively acidic Golgi (9). LRPAP, which contains a C-terminal HNEL motif, can then recycle to the ER (9). A minor amount of LRPAP remains associated with LRP and can modulate receptor activity on the cell surface (5). Exogenously applied LRPAP competitively inhibits LDL receptor family binding and uptake of activated alpha 2-macroglobulin, apoB100- or apoE-enriched LDL and VLDL particles, cholesteryl esters, and complexes of PAI-1 with either tPA or uPA (10‑14).

  • References:
    1. Furukawa, T. et al. (1990) J. Biochem. 108:297. 
    2. Bu, G. (2001) Int. Rev. Cytol. 209:79. 
    3. Lazic, A. et al. (2003) Biochemistry 42:14913. 
    4. Lazic, A. et al. (2006) Arch. Biochem. Biophys. 450:167. 
    5. Bu, G. et al. (1994) J. Biol. Chem. 269:29874. 
    6. Willnow, T.E. et al. (1996) EMBO J. 15:2632. 
    7. Bu, G. and S. Rennke (1996) J. Biol. Chem. 271:22218.
    8. Obermoeller, L.M. et al. (1997) J. Biol. Chem. 272:10761.
    9. Lee, D. et al. (2006) Mol. Cell 22:423.
    10. Williams, S.E. et al. (1992) J. Biol. Chem. 267:9035.
    11. Medh, J.D. et al. (1995) J. Biol. Chem. 270:536.
    12. Herz, J. et al. (1991) J. Biol. Chem. 266:21232.
    13. Mokuno, H. et al. (1994) J. Biol. Chem. 269:13238.
    14. Orth, K. et al. (1992) Proc. Natl. Acad. Sci. 89:7422.
  • Long Name:
    Low Density Lipoprotein-Related Protein-associated Protein
  • Entrez Gene IDs:
    4043 (Human); 16976 (Mouse); 116565 (Rat)
  • Alternate Names:
    A2MRAP; A2MRAPMRAP; A2RAP; alpha-2-macroglobulin receptor-associated protein 1; alpha-2-macroglobulin receptor-associated protein; alpha-2-MRAP; HBP44; lipoprotein receptor associated protein; low density lipoprotein receptor-related protein associated protein 1; Low density lipoprotein receptor-related protein-associated protein 1; low density lipoprotein-related protein-associated protein 1(alpha-2-macroglobulin receptor-associated protein 1); LRPAP; LRPAP1; MGC138272; RAP
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. Low-density lipoprotein receptor represents an apolipoprotein E-independent pathway of Abeta uptake and degradation by astrocytes.
    Authors: Basak JM, Verghese PB, Yoon H, Kim J, Holtzman DM
    J. Biol. Chem., 2012;287(17):13959-71.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
Isotype Controls
Description Application Cat# Citations Images  

Normal Sheep IgG Control

Ctrl 5-001-A 9
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Secondary Antibodies
Description Application Cat# Citations Images  

Sheep IgG HRP-conjugated Antibody

WB, Simple Western HAF016 14  
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Donkey Anti-Sheep IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL010 3
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Donkey Anti-Sheep IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL011 5
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Donkey Anti-Sheep IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL012 2
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Sheep IgG (H+L) PE-conjugated Antibody

Flow F0126  
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Donkey Anti-Sheep IgG Biotinylated Antibody

WB BAF016 2
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Sheep IgG (H+L) APC-conjugated Antibody

Flow F0127  
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Sheep IgG VisUCyte HRP Polymer Antibody

IHC VC006  
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Donkey Anti-Sheep IgG (H+L) Fluorescein-conjugated Antibody

Flow F0125 1
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Donkey Anti-Sheep IgG (H+L) PerCP-conjugated Antibody

Flow F0128
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