Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse, Transgenic Mouse

Applications

Validated:

Flow Cytometry, CyTOF-ready

Cited:

Immunohistochemistry, Neutralization, Flow Cytometry, In vivo assay, Neutrophil Depletion

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2B Clone # 1A8
View all Ly-6G (Gr-1) Primary Antibodies »

Product Specifications

Immunogen

Ly-6G transfected EL-4J cell line
Accession # P35461

Specificity

Detects mouse Ly-6G in Flow Cytometry.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Scientific Data Images for Mouse Ly-6G (Gr-1) Antibody

Detection of Ly-6G (GR-1) antibody in Mouse Bone Marrow Cells antibody by Flow Cytometry

Detection of Ly-6G (GR-1) in Mouse Bone Marrow Cells by Flow Cytometry

Mouse bone marrow cells were stained with either (A) Rat Anti-Mouse Ly-6g (GR-1) Monoclonal Antibody (Catalog # MAB10371) or (B) Rat IgG2A Isotype Control (Catalog # MAB006) followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B) and Rat Anti-Mouse B220/CD45R APC-conjugated Monoclonal Antibody (Catalog # FAB1217A).View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse Ly-6G (Gr-1) Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: Mouse bone marrow cells

Flow Cytometry Panel Builder

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Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Ly-6G (Gr-1)

The myeloid differentiation antigen Gr-1, also known as Ly-6G, is a member of the Ly-6 family (1). The 1A8 antibody binds to Ly-6G while the RB6‑8C5 antibody also reacts weakly with Ly-6C-transfected EL4 cells (2). Ly-6G is a 21-25 kDa glycosyolphosphatidylinositol-anchored protein that is predominatly expressed in granulocytes in bone marrow.

References

  1. Spangrude, G.J. et al. (1988) Science 241:58.
  2. Fleming, T.J. et al. (1993) J. Immunol. 151:2399.

Long Name

A Myeloid Differentiation Antigen

Alternate Names

Gr-1

Entrez Gene IDs

546644 (Mouse)

Gene Symbol

LY6G

UniProt

P35461

Additional Ly-6G (Gr-1) Products

Product Documents for Mouse Ly-6G (Gr-1) Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse Ly-6G (Gr-1) Antibody

For research use only

Citations for Mouse Ly-6G (Gr-1) Antibody

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