Mouse Ly-6G (Gr-1) Antibody

Catalog # Availability Size / Price Qty
MAB10371-100
MAB10371-SP
Detection of Ly-6G (GR-1) in Mouse Bone Marrow Cells by Flow Cytometry
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Product Details
Citations (13)
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Mouse Ly-6G (Gr-1) Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Ly-6G in Flow Cytometry.
Source
Monoclonal Rat IgG2B Clone # 1A8
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Ly-6G transfected EL-4J cell line
Accession # P35461
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Ly-6G (GR-1) antibody in Mouse Bone Marrow Cells antibody by Flow Cytometry View Larger

Detection of Ly-6G (GR-1) in Mouse Bone Marrow Cells by Flow Cytometry Mouse bone marrow cells were stained with either (A) Rat Anti-Mouse Ly-6g (GR-1) Monoclonal Antibody (Catalog # MAB10371) or (B) Rat IgG2A Isotype Control (Catalog # MAB006) followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B) and Rat Anti-Mouse B220/CD45R APC-conjugated Monoclonal Antibody (Catalog # FAB1217A).View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Ly-6G (Gr-1)

The myeloid differentiation antigen Gr-1, also known as Ly-6G, is a member of the Ly-6 family (1). The 1A8 antibody binds to Ly-6G while the RB6‑8C5 antibody also reacts weakly with Ly-6C-transfected EL4 cells (2). Ly-6G is a 21-25 kDa glycosyolphosphatidylinositol-anchored protein that is predominatly expressed in granulocytes in bone marrow.

References
  1. Spangrude, G.J. et al. (1988) Science 241:58.
  2. Fleming, T.J. et al. (1993) J. Immunol. 151:2399.
Long Name
A Myeloid Differentiation Antigen
Entrez Gene IDs
546644 (Mouse)
Alternate Names
GR; Gr1; Gr-1; Ly-6; Ly6G; Ly-6G

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Citations for Mouse Ly-6G (Gr-1) Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

13 Citations: Showing 1 - 10
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  1. Glucocorticoids target the CXCL9/10-CXCR3 axis and confer protection against immune-mediated kidney injury
    Authors: JH Riedel, L Robben, HJ Paust, Y Zhao, N Asada, N Song, A Peters, A Kaffke, AC Borchers, G Tiegs, L Seifert, NM Tomas, E Hoxha, UO Wenzel, TB Huber, T Wiech, JE Turner, CF Krebs, U Panzer
    JCI Insight, 2023-01-10;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Essential Role of NLRP3 Inflammasome in Mediating IL-1beta Production and the Pathobiology of Staphylococcus aureus Endophthalmitis
    Authors: A Kumar, PK Singh, Z Ahmed, S Singh, A Kumar
    Infection and Immunity, 2022-04-11;0(0):e0010322.
    Species: Mouse, Transgenic Mouse
    Sample Types: In Vivo, Whole Cells
    Applications: Flow Cytometry, In Vivo
  3. Anti-Inflammatory Activity of Orally Administered Monostroma nitidum Rhamnan Sulfate against Lipopolysaccharide-Induced Damage to Mouse Organs and Vascular Endothelium
    Authors: M Terasawa, K Hiramoto, R Uchida, K Suzuki
    Marine Drugs, 2022-02-03;20(2):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  4. The Role of Fucoidans Isolated from the Sporophylls of Undaria pinnatifida against Particulate-Matter-Induced Allergic Airway Inflammation: Evidence of the Attenuation of Oxidative Stress and Inflammatory Responses
    Authors: KHINM Herath, HJ Kim, A Kim, CE Sook, BY Lee, Y Jee
    Molecules, 2020-06-22;25(12):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  5. Pathobiology of Aspergillus Fumigatus Endophthalmitis in Immunocompetent and Immunocompromised Mice
    Authors: N Gupta, PK Singh, SG Revankar, PH Chandrasek, A Kumar
    Microorganisms, 2019-08-28;7(9):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutrophil Depletion
  6. Unique role for ATG5 in neutrophil-mediated immunopathology during M. tuberculosis infection.
    Authors: Kimmey J, Huynh J, Weiss L, Park S, Kambal A, Debnath J, Virgin H, Stallings C
    Nature, 2015-12-09;528(7583):565-9.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  7. Antibodies against neutrophil LY6G do not inhibit leukocyte recruitment in mice in vivo.
    Authors: Yipp B, Kubes P
    Blood, 2013-01-03;121(1):241-2.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  8. Critical role for MyD88-mediated neutrophil recruitment during Clostridium difficile colitis.
    Authors: Jarchum I, Liu M, Shi C, Equinda M, Pamer E
    Infect Immun, 2012-06-11;80(9):2989-96.
    Species: Mouse
    Sample Types: In Vivo, Whole Tissue
    Applications: IHC, Neutralization
  9. The role of neutrophils during mild and severe influenza virus infections of mice.
    Authors: Tate M, Ioannidis L, Croker B, Brown L, Brooks A, Reading P
    PLoS ONE, 2011-03-14;6(3):e17618.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  10. Depletion of Gr-1+, but not Ly6G+, immune cells exacerbates virus replication and disease in an intranasal model of herpes simplex virus type 1 infection.
    Authors: Wojtasiak M, Pickett D, Tate M, Londrigan S, Bedoui S, Brooks A, Reading P
    J Gen Virol, 2010-06-09;91(0):2158-66.
    Species: Mouse
    Sample Types: In Vivo, Whole Cells
    Applications: Flow Cytometry, Neutralization
  11. Inflammatory monocytes but not neutrophils are necessary to control infection with Toxoplasma gondii in mice.
    Authors: Dunay I, Fuchs A, Sibley L
    Infect Immun, 2010-02-09;78(4):1564-70.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  12. Neutrophils ameliorate lung injury and the development of severe disease during influenza infection.
    Authors: Tate M, Deng Y, Jones J, Anderson G, Brooks A, Reading P
    J Immunol, 2009-11-16;183(11):7441-50.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  13. Use of Ly6G-specific monoclonal antibody to deplete neutrophils in mice.
    Authors: Daley J, Thomay A, Connolly M, Reichner J, Albina J
    J Leukoc Biol, 2007-09-20;83(1):64-70.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization

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