Mouse M-CSF Quantikine ELISA Kit

(14 citations)   
  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (13 uL), Serum (13 uL), EDTA Plasma (13 uL)
  • Sensitivity
    5 pg/mL
  • Assay Range
    31.2 - 2,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma)
  • Specificity
    Natural and recombinant mouse M-CSF
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.
Product Summary
The Quantikine Mouse M-CSF Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse M-CSF levels in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant mouse M-CSF and antibodies raised against the recombinant factor. Results obtained for naturally occurring mouse M-CSF showed linear curves that were parallel to the standard curves obtained using the kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse M-CSF.

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum, EDTA Plasma
Intra-Assay Precision Inter-Assay Precision
Standard Deviation3.910.238.9410.135.2


The recovery of mouse M-CSF spiked to three levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Supernates (n=5) 104 85-120
EDTA Plasma (n=6) 101 89-112
Serum (n=6) 97 85-115
To assess the linearity of the assay, five or more samples containing and/or spiked with various concentrations of mouse M-CSF in each matrix were diluted with Calibrator Diluent and then assayed.
Mouse M-CSF Quantikine ELISA Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: M-CSF
M-CSF, also known as CSF-1, is a four-alpha-helical-bundle cytokine that is the primary regulator of macrophage survival, proliferation and differentiation. M-CSF is also essential for the survival and proliferation of osteoclast progenitors. M-CSF also primes and enhances macrophage killing of tumor cells and microorganisms, regulates the release of cytokines and other inflammatory modulators from macrophages, and stimulates pinocytosis. M-CSF increases during pregnancy to support implantation and growth of the decidua and placenta. Sources of M-CSF include fibroblasts, activated macrophages, endometrial secretory epithelium, bone marrow stromal cells and activated endothelial cells. The M-CSF receptor (c-fms) transduces its pleotropic effects and mediates its endocytosis. M-CSF mRNAs of various sizes occur. Full length human M-CSF transcripts encode a 522 amino acid (aa) type I transmembrane (TM) protein with a 464 aa extracellular region, a 21 aa TM domain, and a 37 aa cytoplasmic tail that forms a 140 kDa covalent dimer. Differential processing produces two proteolytically cleaved, secreted dimers. One is an N- and O- glycosylated 86 kDa dimer, while the other is modified by both glycosylation and chondroitin-sulfate proteoglycan (PG) to generate a 200 kDa subunit. Although PG-modified M-CSF can circulate, it may be immobilized by attachment to type V collagen. Shorter transcripts encode M-CSF that lack cleavage and PG sites and produce an N-glycosylated 68 kDa TM dimer and a slowly produced 44 kDa secreted dimer. Although forms may vary in activity and half-life, all contain the N-terminal 150 aa portion that is necessary and sufficient for interaction with the M-CSF receptor. The first 223 aa of mature human M-CSF shares 88%, 86%, 81% and 74% aa identity with corresponding regions of dog, cow, mouse and rat M-CSF, respectively. Human M-CSF is active in the mouse, but mouse M-CSF is reported to be species-specific.
    • Long Name
      Macrophage Colony Stimulating Factor
    • Entrez Gene IDs
      1435 (Human); 12977 (Mouse);
    • Alternate Names
      colony stimulating factor 1 (macrophage); CSF1; CSF-1; Lanimostim; macrophage colony stimulating factor; macrophage colony-stimulating factor 1; MCSF; M-CSF; MCSFlanimostim; MGC31930;
    Related Research Areas
    Assay Procedure
    Refer to the product for complete assay procedure.

    Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
    1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
    2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

    3. 50 µL Assay Diluent
    4.   Add 50 µL of Assay Diluent to each well.

    5. 50 µL Standard, Control, or Sample
    6.   Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
    7.   Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.

    8. 100 µL Conjugate
    9.   Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
    10.   Aspirate and wash 5 times.

    11. 100 µL Substrate Solution
    12.   Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

    13. 100 µL Stop Solution
    14.   Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    Showing Results 1 - 10 of 14
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    Sample Type
    1. SHIP1-expressing mesenchymal stem cells regulate hematopoietic stem cell homeostasis and lineage commitment during aging.
      Authors: Iyer S, Brooks R, Gumbleton M, Kerr W
      Stem Cells Dev, 2015;24(9):1073-81.
      Species: Mouse
      Sample Type: Serum
    2. Steady-state neutrophil homeostasis is dependent on TLR4/TRIF signaling.
      Authors: Bugl S, Wirths S, Radsak M, Schild H, Stein P, Andre M, Muller M, Malenke E, Wiesner T, Marklin M, Frick J, Handgretinger R, Rammensee H, Kanz L, Kopp H
      Blood, 2013;121(5):723-33.
      Species: Mouse
      Sample Type: Plasma
    3. KIT oncogene inhibition drives intratumoral macrophage M2 polarization.
      Authors: Cavnar, Michael, Zeng, Shan, Kim, Teresa S, Sorenson, Eric C, Ocuin, Lee M, Balachandran, Vinod P, Seifert, Adrian M, Greer, Jonathan, Popow, Rachel, Crawley, Megan H, Cohen, Noah A, Green, Benjamin, Rossi, Ferdinan, Besmer, Peter, Antonescu, Cristina, DeMatteo, Ronald P
      J Exp Med, 2013;210(13):2873-86.
      Species: Mouse
      Sample Type: Serum
    4. The combination of the histone deacetylase inhibitor vorinostat and synthetic triterpenoids reduces tumorigenesis in mouse models of cancer.
      Authors: Tran, Kim, Risingsong, Renee, Royce, Darlene, Williams, Charlott, Sporn, Michael, Pioli, Patricia, Gediya, Lalji K, Njar, Vincent, Liby, Karen T
      Carcinogenesis, 2013;34(1):199-210.
      Species: Mouse
      Sample Type: cell supernates
    5. Osteoclasts are dispensable for hematopoietic stem cell maintenance and mobilization.
      Authors: Miyamoto K, Yoshida S, Kawasumi M
      J. Exp. Med., 2011;208(11):2175-81.
      Species: Mouse
      Sample Type: Serum
    6. Temporal changes in myeloid cells in the cervix during pregnancy and parturition.
      Authors: Timmons BC, Fairhurst AM, Mahendroo MS
      J. Immunol., 2009;182(5):2700-7.
      Species: Mouse
      Sample Type: Tissue Homogenates
    7. A potential role of thymic stromal lymphopoietin in the recruitment of macrophages to mouse intervertebral disc cells via monocyte chemotactic protein 1 induction: implications for herniated discs.
      Authors: Ohba T, Haro H, Ando T, Koyama K, Hatsushika K, Suenaga F, Ohnuma Y, Nakamura Y, Katoh R, Ogawa H, Hamada Y, Nakao A
      Arthritis Rheum., 2008;58(11):3510-9.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    8. IL-7 induces myelopoiesis and erythropoiesis.
      Authors: Aiello FB, Keller JR, Klarmann KD, Dranoff G, Mazzucchelli R, Durum SK
      J. Immunol., 2007;178(3):1553-63.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    9. Bacterial lipopolysaccharide induces osteoclast formation in RAW 264.7 macrophage cells.
      Authors: Islam S, Hassan F, Tumurkhuu G, Dagvadorj J, Koide N, Naiki Y, Mori I, Yoshida T, Yokochi T
      Biochem. Biophys. Res. Commun., 2007;360(2):346-51.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    10. Concerted action of Smad and CREB-binding protein regulates bone morphogenetic protein-2-stimulated osteoblastic colony-stimulating factor-1 expression.
      Authors: Ghosh-Choudhury N, Singha PK, Woodruff K, St Clair P, Bsoul S, Werner SL, Choudhury GG
      J. Biol. Chem., 2006;281(29):20160-70.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    11. Perforin-deficient CD8+ T cells mediate fatal lymphocytic choriomeningitis despite impaired cytokine production.
      Authors: Storm P, Bartholdy C, Sorensen MR, Christensen JP, Thomsen AR
      J. Virol., 2006;80(3):1222-30.
      Species: Mouse
      Sample Type: Serum
    12. Interleukin-17 as a recruitment and survival factor for airway macrophages in allergic airway inflammation.
      Authors: Sergejeva S, Ivanov S, Lotvall J, Linden A
      Am. J. Respir. Cell Mol. Biol., 2005;33(3):248-53.
      Species: Mouse
      Sample Type: BALF
    13. The cell-surface isoform of colony stimulating factor 1 (CSF1) restores but does not completely normalize fecundity in CSF1-deficient mice.
      Authors: Ovadia S, Insogna K, Yao GQ
      Biol. Reprod., 2005;74(2):331-6.
      Species: Mouse
      Sample Type: Tissue Homogenates
    14. Differential roles of CC chemokine ligand 2/monocyte chemotactic protein-1 and CCR2 in the development of T1 immunity.
      Authors: Traynor TR, Herring AC, Dorf ME, Kuziel WA, Toews GB, Huffnagle GB
      J. Immunol., 2002;168(9):4659-66.
      Species: Mouse
      Sample Type: BALF
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