Mouse MCAM/CD146 APC-conjugated Antibody

FAB7718A has been discontinued. View all MCAM/CD146 products.
    
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  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse MCAM/CD146 in direct ELISAs. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse (rm) MAdCAM-1 is observed, and no cross-reactivity with rmALCAM, rmNCAM, rmL1CAM, rmOCAM, rmTROP-2, recombinant human MCAM, or recombinant rat MCAM is observed.
  • Source
    Monoclonal Rat IgG2A Clone # 733216
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse MCAM/CD146
    Met1-Val563
    Accession # Q8R2Y2
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Allophycocyanin
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Detection of MCAM/CD146 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse CD161/NK1.1 PE‑conjugated Monoclonal Antibody (Catalog # FAB7614P) and either (A) Rat Anti-Mouse MCAM/CD146 APC‑conjugated Monoclonal Antibody (Catalog # FAB7718A) or (B) Rat IgG2A Allophycocyanin Isotype Control (Catalog # IC006A). View our protocol for Staining Membrane-associated Proteins
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: MCAM/CD146

MCAM (Melanoma Cell Adhesion Molecule), also known as CD146 and MUC18, is a 110-120 kDa member of a small group of Ig-superfamily molecules that includes CD239 and CD166. In rodents, MCAM is reportedly expressed on neurons, endothelial cells, NK cells, neutrophils, mesenchymal stem cells and melanoma cells. MCAM appears to contribute to intercellular endothelial cell junctions, and possibly contributes to the migration of select cell types. It has been suggested that CD146 may act homotypically, or contribute to a Fzd:Dv12:CD146 Wnt receptor complex. Mature mouse MCAM is a 625 amino acid (aa) type I transmembrane glycoprotein. Its extracellular region is 540 aa in length (aa 24-563). It contains two V-type Ig-like domains (aa 24-244) followed by three C2-type Ig-like domains (aa 246-512). One cytoplasmic region splice form shows a seven aa substitution for aa 600-648. Unlike human, rodent MCAM does not undergo a splicing event that will generate a soluble isoform. Over aa 24-563, mouse MCAM shares 90% and 74% aa identity with rat and human MCAM, respectively.

  • Long Name:
    Melanoma Cell Adhesion Molecule
  • Entrez Gene IDs:
    4162 (Human); 84004 (Mouse); 78967 (Rat)
  • Alternate Names:
    CD146 antigen; CD146; cell surface glycoprotein MUC18; Cell surface glycoprotein P1H12; L-Gicerin; MCAM; melanoma adhesion molecule; melanoma cell adhesion moleculeS-endo 1 endothelial-associated antigen; Melanoma-associated antigen A32; Melanoma-associated antigen MUC18; MUC18; MUC18Gicerin
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