Detects mouse MCAM/CD146 in direct ELISAs.
In direct ELISAs, less than 5%
cross-reactivity with recombinant mouse (rm) MAdCAM-1 is observed, and no
cross-reactivity with rmALCAM, rmNCAM, rmL1CAM, rmOCAM, rmTROP-2, recombinant
human MCAM, or recombinant rat MCAM is observed.
Monoclonal Rat IgG2A Clone # 733216
Protein A or G purified from hybridoma culture supernatant
Detection of MCAM/CD146 in Mouse Splenocytes by Flow Cytometry.
Mouse splenocytes were stained with Rat Anti-Mouse CD161/NK1.1 PE‑conjugated Monoclonal Antibody (Catalog # FAB7614P) and either (A) Rat Anti-Mouse MCAM/CD146 APC‑conjugated Monoclonal Antibody (Catalog # FAB7718A) or (B) Rat IgG2A Allophycocyanin Isotype Control (Catalog # IC006A). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
MCAM (Melanoma Cell Adhesion Molecule), also known as CD146 and MUC18, is a 110-120 kDa member of a small group of Ig-superfamily molecules that includes CD239 and CD166. In rodents, MCAM is reportedly expressed on neurons, endothelial cells, NK cells, neutrophils, mesenchymal stem cells and melanoma cells. MCAM appears to contribute to intercellular endothelial cell junctions, and possibly contributes to the migration of select cell types. It has been suggested that CD146 may act homotypically, or contribute to a Fzd:Dv12:CD146 Wnt receptor complex. Mature mouse MCAM is a 625 amino acid (aa) type I transmembrane glycoprotein. Its extracellular region is 540 aa in length (aa 24-563). It contains two V-type Ig-like domains (aa 24-244) followed by three C2-type Ig-like domains (aa 246-512). One cytoplasmic region splice form shows a seven aa substitution for aa 600-648. Unlike human, rodent MCAM does not undergo a splicing event that will generate a soluble isoform. Over aa 24-563, mouse MCAM shares 90% and 74% aa identity with rat and human MCAM, respectively.
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