Mouse MEPE/OF45 Antibody

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Detection of Mouse MEPE/OF45 by Western Blot.
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Product Details
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Mouse MEPE/OF45 Antibody Summary

Species Reactivity
Detects mouse MEPE/OF45 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human MEPE/OF45 is observed.
Polyclonal Sheep IgG
Antigen Affinity-purified
Mouse myeloma cell line NS0-derived recombinant mouse MEPE/OF45
Accession # AAK70342
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1.0 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse MEPE/OF45 antibody by Western Blot. View Larger

Detection of Mouse MEPE/OF45 by Western Blot. Western blot shows lysates of ST-2 mouse bone marrow-derived stromal cell line and mouse embryo tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse MEPE/OF45 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6150) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for MEPE/OF45 at approximately 58 and 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

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Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MEPE/OF45

MEPE (matrix extracellular phosphoglycoprotein; also OF45) is a 56‑58 kDa member of the SIBLING protein family. It is expressed by osteocytes, osteoblasts, odontoblasts and chondrocytes. MEPE is found both intra- and extracellularly. Within the cell, it binds to CHK1. This protects CHK1 from degradation, and the cell from DNA damage. Extracellularly, it binds to Phex, which protects it from cathepsin B proteolysis. Undegraded, it promotes bone vascularization and blocks bone resorption. Following cleavage after Arg410 (in mouse), a 2 kDa ASARM fragment is generated that, when phosphorylated, blocks mineralization. Mature mouse MEPE is 417 amino acids (aa) in length. It contains an RGD motif and ASARM domain (aa 416‑433). In addition to its a 57 kDa form, 70 kDa, 42‑45 kDa and 30‑35 kDa forms of mouse MEPE have been reported. Whether they are cleavage forms or products of glycosylation is unclear. Over aa 147‑433, mouse MEPE shares 77% and 57% aa identity with rat and human MEPE, respectively.

Long Name
Matrix Extracellular Phosphoglycoprotein with ASARM Motif
Entrez Gene IDs
56955 (Human); 94111 (Mouse); 79110 (Rat)
Alternate Names
extracellular phosphoglycoprotein with ASARM motif (bone); matrix extracellular phosphoglycoprotein; MEPE; OF45; Osteoregulin

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