Mouse MIF DuoSet ELISA

DY1978 5 Citations Datasheet / COA / SDS

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DY008: DuoSet ELISA Ancillary Reagent Kit 2

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Summary Product Features Kit Content Other Reagents Required Data Examples Product Datasheets Preparation and Storage Background

Mouse MIF DuoSet ELISA Summary

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Sample Volume Required

100 µL

Sufficient Materials

For fifteen 96-well plates*

Specificity

Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant mouse MIF. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet ELISA.

Product Features

Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
Development protocols are provided to guide further assay optimization
Assay can be customized to your specific needs
Economical alternative to complete kits

Kit Content

Capture Antibody
Detection Antibody
Recombinant Standard
Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na₂HPO₄, 1.5 mM KH₂PO₄, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Reagent Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H₂O₂) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H₂SO₄ (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product .

Data Example

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Mouse MIF ELISA Standard Curve

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: MIF

MIF (macrophage migration inhibitory factor) is a pro-inflammatory cytokine that is released during microbial infection, ARDS, rheumatoid arthritis, atherosclerosis, type 2 diabetes, and cancer. It signals through CXCR4, CXCR7, or complexes of CD74-CD44. MIF exhibits potent paracrine and autocrine stimulatory effects on cell growth and survival and enhances the production of inflammatory cytokines. It also inhibits the effectiveness of immunosuppressive glucocorticoids.

Long Name:

Macrophage Migration Inhibitory Factor

Entrez Gene IDs:

4282 (Human); 17319 (Mouse)

Alternate Names:

EC 5.3.2.1; EC 5.3.3.12; GIFmacrophage migration inhibitory factor; GLIF; Glycosylation-inhibiting factor; L-dopachrome isomerase; L-dopachrome tautomerase; macrophage migration inhibitory factor (glycosylation-inhibiting factor); MIF; MMIF; Phenylpyruvate tautomerase

Citations for Mouse MIF DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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Involvement of HPV Infection in the Release of Macrophage Migration Inhibitory Factor in Head and Neck Squamous Cell Carcinoma
Authors: N Kindt, G Descamps, JR Lechien, M Remmelink, JM Colet, R Wattiez, G Berchem, F Journe, S Saussez
J Clin Med, 2019;8(1):.
Species: Mouse
Sample Types: Serum
Macrophage migration inhibitory factor enhances influenza-associated mortality in mice
Authors: CA Smith, DJ Tyrell, UA Kulkarni, S Wood, L Leng, RL Zemans, R Bucala, DR Goldstein
JCI Insight, 2019;4(13):.
Species: Mouse
Sample Types: BALF
Macrophage Migration Inhibitory Factor Promotes the Interaction between the Tumor, Macrophages, and T Cells to Regulate the Progression of Chemically Induced Colitis-Associated Colorectal Cancer
Authors: T Pacheco-Fe, I Juárez-Ave, O Illescas, LI Terrazas, R Hernández-, C Pérez-Plas, EB Gutiérrez-, F Ávila-More, YI Chirino, JL Reyes, V Maldonado, M Rodriguez-
Mediators Inflamm., 2019;2019(0):2056085.
Species: Mouse
Sample Types: Serum
Proinflammatory cytokine MIF plays a role in the pathogenesis of type-2 diabetes mellitus, but does not affect hepatic mitochondrial function
Authors: M Rodriguez-, T Cabellos-A, Y Sanchez-Za, I Juárez-Ave, E García-Rey, A Lira-León, JDC Benítez-Fl, T Pacheco-Fe, M Hiriart, EB Gutiérrez-
Cytokine, 2017;0(0):.
Species: Mouse
Sample Types: Serum
MIF Inhibitor ISO-1 Protects Photoreceptors and Reduces Gliosis in Experimental Retinal Detachment
Authors: B Kim, R Kusibati, T Heisler-Ta, D Mantopoulo, J Ding, MH Abdel-Rahm, AR Satoskar, JP Godbout, SK Bhattachar, CM Cebulla
Sci Rep, 2017;7(1):14336.
Species: Mouse
Sample Types: Complex Sample Type