Mouse MIF DuoSet ELISA
Mouse MIF DuoSet ELISA Summary
* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.
Product Features
- Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
- Development protocols are provided to guide further assay optimization
- Assay can be customized to your specific needs
- Economical alternative to complete kits
Kit Content
- Capture Antibody
- Detection Antibody
- Recombinant Standard
- Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)
Other Reagents Required
PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered
Wash Buffer: (Catalog # WA126), or equivalent
Reagent Diluent*
Blocking Buffer*
Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)
Stop Solution: 2 N H2SO4 (Catalog # DY994)
Microplates: R&D Systems (Catalog # DY990), or equivalent
Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent
*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product
Data Example
Product Datasheets
Preparation and Storage
Background: MIF
MIF (macrophage migration inhibitory factor) is a pro-inflammatory cytokine that is released during microbial infection, ARDS, rheumatoid arthritis, atherosclerosis, type 2 diabetes, and cancer. It signals through CXCR4, CXCR7, or complexes of CD74-CD44. MIF exhibits potent paracrine and autocrine stimulatory effects on cell growth and survival and enhances the production of inflammatory cytokines. It also inhibits the effectiveness of immunosuppressive glucocorticoids.
Citations for Mouse MIF DuoSet ELISA
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 6
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Autophagy Inhibition Induces the Secretion of Macrophage Migration Inhibitory Factor (MIF) with Autocrine and Paracrine Effects on the Promotion of Malignancy in Breast Cancer
Authors: I Cotzomi-Or, A Rosas-Cruz, D Ramírez-Ra, J Reyes-Leyv, M Rodriguez-, P Aguilar-Al, P Maycotte
Biology (Basel), 2020;9(1):.
Species: Mouse
Sample Types: Cell Cultue Supernates
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Involvement of HPV Infection in the Release of Macrophage Migration Inhibitory Factor in Head and Neck Squamous Cell Carcinoma
Authors: N Kindt, G Descamps, JR Lechien, M Remmelink, JM Colet, R Wattiez, G Berchem, F Journe, S Saussez
J Clin Med, 2019;8(1):.
Species: Mouse
Sample Types: Serum
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Macrophage migration inhibitory factor enhances influenza-associated mortality in mice
Authors: CA Smith, DJ Tyrell, UA Kulkarni, S Wood, L Leng, RL Zemans, R Bucala, DR Goldstein
JCI Insight, 2019;4(13):.
Species: Mouse
Sample Types: BALF
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Macrophage Migration Inhibitory Factor Promotes the Interaction between the Tumor, Macrophages, and T Cells to Regulate the Progression of Chemically Induced Colitis-Associated Colorectal Cancer
Authors: T Pacheco-Fe, I Juárez-Ave, O Illescas, LI Terrazas, R Hernández-, C Pérez-Plas, EB Gutiérrez-, F Ávila-More, YI Chirino, JL Reyes, V Maldonado, M Rodriguez-
Mediators Inflamm., 2019;2019(0):2056085.
Species: Mouse
Sample Types: Serum
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Proinflammatory cytokine MIF plays a role in the pathogenesis of type-2 diabetes mellitus, but does not affect hepatic mitochondrial function
Authors: M Rodriguez-, T Cabellos-A, Y Sanchez-Za, I Juárez-Ave, E García-Rey, A Lira-León, JDC Benítez-Fl, T Pacheco-Fe, M Hiriart, EB Gutiérrez-
Cytokine, 2017;0(0):.
Species: Mouse
Sample Types: Serum
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MIF Inhibitor ISO-1 Protects Photoreceptors and Reduces Gliosis in Experimental Retinal Detachment
Authors: B Kim, R Kusibati, T Heisler-Ta, D Mantopoulo, J Ding, MH Abdel-Rahm, AR Satoskar, JP Godbout, SK Bhattachar, CM Cebulla
Sci Rep, 2017;7(1):14336.
Species: Mouse
Sample Types: Complex Sample Type
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