Mouse MIF DuoSet ELISA

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Mouse MIF ELISA Standard Curve
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Product Details
Citations (14)
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Mouse MIF DuoSet ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
96-well strip plate
Sample Volume Required
100 µL
Assay Range
125.0 - 8,000 pg/mL
Sufficient Materials
For fifteen 96-well plates*
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant mouse MIF. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet ELISA.

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Kit Content

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Reagent Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product .

Scientific Data

Mouse MIF ELISA Standard Curve

Product Datasheets

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Preparation and Storage

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: MIF

MIF (macrophage migration inhibitory factor) is a pro-inflammatory cytokine that is released during microbial infection, ARDS, rheumatoid arthritis, atherosclerosis, type 2 diabetes, and cancer. It signals through CXCR4, CXCR7, or complexes of CD74-CD44. MIF exhibits potent paracrine and autocrine stimulatory effects on cell growth and survival and enhances the production of inflammatory cytokines. It also inhibits the effectiveness of immunosuppressive glucocorticoids.

Long Name:
Macrophage Migration Inhibitory Factor
Entrez Gene IDs:
4282 (Human); 17319 (Mouse)
Alternate Names:
EC; EC; GIFmacrophage migration inhibitory factor; GLIF; Glycosylation-inhibiting factor; L-dopachrome isomerase; L-dopachrome tautomerase; macrophage migration inhibitory factor (glycosylation-inhibiting factor); MIF; MMIF; Phenylpyruvate tautomerase

Citations for Mouse MIF DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

14 Citations: Showing 1 - 10
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  1. Inducing trained immunity in pro-metastatic macrophages to control tumor metastasis
    Authors: C Ding, R Shrestha, X Zhu, AE Geller, S Wu, MR Woeste, W Li, H Wang, F Yuan, R Xu, JH Chariker, X Hu, H Li, D Tieri, HG Zhang, EC Rouchka, R Mitchell, LJ Siskind, X Zhang, XG Xu, KM McMasters, Y Yu, J Yan
    Nature Immunology, 2023-01-05;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  2. Therapeutic Intervention with Anti-Complement Component 5 Antibody Does Not Reduce NASH but Does Attenuate Atherosclerosis and MIF Concentrations in Ldlr-/-.Leiden Mice
    Authors: F Seidel, R Kleemann, W van Duyven, N van Trigt, N Keijzer, S van der Ko, C van Kooten, L Verschuren, A Menke, AJ Kiliaan, J Winter, TR Hughes, BP Morgan, F Baas, K Fluiter, MC Morrison
    International Journal of Molecular Sciences, 2022-09-14;23(18):.
    Species: Mouse
    Sample Types: Plasma
  3. Heat-Inactivated Akkermansia muciniphila Improves Gut Permeability but Does Not Prevent Development of Non-Alcoholic Steatohepatitis in Diet-Induced Obese Ldlr-/-.Leiden Mice
    Authors: MC Morrison, E Gart, WV Duyvenvoor, J Snabel, MJ Nielsen, DJ Leeming, A Menke, R Kleemann
    International Journal of Molecular Sciences, 2022-02-19;23(4):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  4. Autophagy inhibition in breast cancer cells induces ROS-mediated MIF expression and M1 macrophage polarization
    Authors: I Cotzomi-Or, O Nieto-Yañe, I Juárez-Ave, G Rojas-Sánc, JB Montes-Alv, J Reyes-Leyv, P Aguilar-Al, M Rodríguez-, P Maycotte
    Cellular Signalling, 2021-07-03;0(0):110075.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  5. A high docosahexaenoic acid diet alters lung inflammation and recovery following repetitive exposure to aqueous organic dust extracts: Role of DHA in repetitive organic dust exposure
    Authors: A Ulu, A Burr, AJ Heires, J Pavlik, T Larsen, PA Perez, C Bravo, NV DiPatrizio, M Baack, DJ Romberger, TM Nordgren
    The Journal of nutritional biochemistry, 2021-06-12;0(0):108797.
    Species: Mouse
    Sample Types: BALF
  6. Macrophage miR-210 induction and metabolic reprogramming in response to pathogen interaction boost life-threatening inflammation
    Authors: F Virga, F Cappelless, B Stijlemans, AT Henze, R Trotta, J Van Audena, AS Mirchandan, MA Sanchez-Ga, J Vandewalle, F Orso, C Riera-Domi, A Griffa, C Ivan, E Smits, D Laoui, F Martelli, L Langouche, G Van den Be, O Feron, B Ghesquière, H Prenen, C Libert, SR Walmsley, C Corbet, JA Van Ginder, M Ivan, D Taverna, M Mazzone
    Science Advances, 2021-05-07;7(19):.
    Species: Mouse
    Sample Types: Plasma
  7. Proteomics reveals distinct mechanisms regulating the release of cytokines and alarmins during pyroptosis
    Authors: K Phulphagar, LI Kühn, S Ebner, A Frauenstei, JJ Swietlik, J Rieckmann, F Meissner
    Cell Reports, 2021-03-09;34(10):108826.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  8. Simvastatin Posttreatment Controls Inflammation and Improves Bacterial Clearance in Experimental Sepsis
    Authors: FM de Jesus O, CF Gonçalves-, IMM de Moraes, PA Reis, VN Rocha, PT Bozza, AR Silva, HC de Castro
    Mediators Inflamm, 2020-10-14;2020(0):1839762.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  9. Autophagy Inhibition Induces the Secretion of Macrophage Migration Inhibitory Factor (MIF) with Autocrine and Paracrine Effects on the Promotion of Malignancy in Breast Cancer
    Authors: I Cotzomi-Or, A Rosas-Cruz, D Ramírez-Ra, J Reyes-Leyv, M Rodriguez-, P Aguilar-Al, P Maycotte
    Biology (Basel), 2020-01-18;9(1):.
    Species: Mouse
    Sample Types: Cell Cultue Supernates
  10. Macrophage migration inhibitory factor enhances influenza-associated mortality in mice
    Authors: CA Smith, DJ Tyrell, UA Kulkarni, S Wood, L Leng, RL Zemans, R Bucala, DR Goldstein
    JCI Insight, 2019-07-11;4(13):.
    Species: Mouse
    Sample Types: BALF
  11. Macrophage Migration Inhibitory Factor Promotes the Interaction between the Tumor, Macrophages, and T Cells to Regulate the Progression of Chemically Induced Colitis-Associated Colorectal Cancer
    Authors: T Pacheco-Fe, I Juárez-Ave, O Illescas, LI Terrazas, R Hernández-, C Pérez-Plas, EB Gutiérrez-, F Ávila-More, YI Chirino, JL Reyes, V Maldonado, M Rodriguez-
    Mediators Inflamm., 2019-07-10;2019(0):2056085.
    Species: Mouse
    Sample Types: Serum
  12. Involvement of HPV Infection in the Release of Macrophage Migration Inhibitory Factor in Head and Neck Squamous Cell Carcinoma
    Authors: N Kindt, G Descamps, JR Lechien, M Remmelink, JM Colet, R Wattiez, G Berchem, F Journe, S Saussez
    J Clin Med, 2019-01-10;8(1):.
    Species: Mouse
    Sample Types: Serum
  13. MIF Inhibitor ISO-1 Protects Photoreceptors and Reduces Gliosis in Experimental Retinal Detachment
    Authors: B Kim, R Kusibati, T Heisler-Ta, D Mantopoulo, J Ding, MH Abdel-Rahm, AR Satoskar, JP Godbout, SK Bhattachar, CM Cebulla
    Sci Rep, 2017-10-30;7(1):14336.
    Species: Mouse
    Sample Types: Complex Sample Type
  14. Proinflammatory cytokine MIF plays a role in the pathogenesis of type-2 diabetes mellitus, but does not affect hepatic mitochondrial function
    Authors: M Rodriguez-, T Cabellos-A, Y Sanchez-Za, I Juárez-Ave, E García-Rey, A Lira-León, JDC Benítez-Fl, T Pacheco-Fe, M Hiriart, EB Gutiérrez-
    Cytokine, 2017-08-02;0(0):.
    Species: Mouse
    Sample Types: Serum


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