Mouse MIF DuoSet ELISA
1 Image
Summary
Product Features
Kit Content
Other Reagents Required
Data Examples
Product Datasheets
Preparation and Storage
Background
Mouse MIF DuoSet ELISA Summary
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Volume Required
100 µL
Assay Range
125.0 - 8,000 pg/mL
Sufficient Materials
For fifteen 96-well plates*
Specificity
Please see the product datasheet
* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.
This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant mouse MIF. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet ELISA.
Complete Your Research
Complete Your Experiment
Product Features
- Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
- Development protocols are provided to guide further assay optimization
- Assay can be customized to your specific needs
- Economical alternative to complete kits
Kit Content
- Capture Antibody
- Detection Antibody
- Recombinant Standard
- Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)
Other Reagents Required
PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered
Wash Buffer: (Catalog # WA126), or equivalent
Reagent Diluent*
Blocking Buffer*
Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)
Stop Solution: 2 N H2SO4 (Catalog # DY994)
Microplates: R&D Systems (Catalog # DY990), or equivalent
Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent
*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product
Scientific Data
Product Datasheets
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: MIF
MIF (macrophage migration inhibitory factor) is a pro-inflammatory cytokine that is released during microbial infection, ARDS, rheumatoid arthritis, atherosclerosis, type 2 diabetes, and cancer. It signals through CXCR4, CXCR7, or complexes of CD74-CD44. MIF exhibits potent paracrine and autocrine stimulatory effects on cell growth and survival and enhances the production of inflammatory cytokines. It also inhibits the effectiveness of immunosuppressive glucocorticoids.
Long Name:
Macrophage Migration Inhibitory Factor
Entrez Gene IDs:
4282 (Human); 17319 (Mouse)
Alternate Names:
EC 5.3.2.1; EC 5.3.3.12; GIFmacrophage migration inhibitory factor; GLIF; Glycosylation-inhibiting factor; L-dopachrome isomerase; L-dopachrome tautomerase; macrophage migration inhibitory factor (glycosylation-inhibiting factor); MIF; MMIF; Phenylpyruvate tautomerase
Citations for Mouse MIF DuoSet ELISA
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
12
Citations: Showing 1 - 10
Filter your results:
Filter by:
-
Heat-Inactivated Akkermansia muciniphila Improves Gut Permeability but Does Not Prevent Development of Non-Alcoholic Steatohepatitis in Diet-Induced Obese Ldlr-/-.Leiden Mice
Authors: MC Morrison, E Gart, WV Duyvenvoor, J Snabel, MJ Nielsen, DJ Leeming, A Menke, R Kleemann
International Journal of Molecular Sciences, 2022;23(4):.
Species: Mouse
Sample Types: Tissue Homogenates
-
Autophagy inhibition in breast cancer cells induces ROS-mediated MIF expression and M1 macrophage polarization
Authors: I Cotzomi-Or, O Nieto-Yañe, I Juárez-Ave, G Rojas-Sánc, JB Montes-Alv, J Reyes-Leyv, P Aguilar-Al, M Rodríguez-, P Maycotte
Cellular Signalling, 2021;0(0):110075.
Species: Mouse
Sample Types: Cell Culture Supernates
-
Proteomics reveals distinct mechanisms regulating the release of cytokines and alarmins during pyroptosis
Authors: K Phulphagar, LI Kühn, S Ebner, A Frauenstei, JJ Swietlik, J Rieckmann, F Meissner
Cell Reports, 2021;34(10):108826.
Species: Mouse
Sample Types: Cell Culture Supernates
-
A high docosahexaenoic acid diet alters lung inflammation and recovery following repetitive exposure to aqueous organic dust extracts: Role of DHA in repetitive organic dust exposure
Authors: A Ulu, A Burr, AJ Heires, J Pavlik, T Larsen, PA Perez, C Bravo, NV DiPatrizio, M Baack, DJ Romberger, TM Nordgren
The Journal of nutritional biochemistry, 2021;0(0):108797.
Species: Mouse
Sample Types: BALF
-
Macrophage miR-210 induction and metabolic reprogramming in response to pathogen interaction boost life-threatening inflammation
Authors: F Virga, F Cappelless, B Stijlemans, AT Henze, R Trotta, J Van Audena, AS Mirchandan, MA Sanchez-Ga, J Vandewalle, F Orso, C Riera-Domi, A Griffa, C Ivan, E Smits, D Laoui, F Martelli, L Langouche, G Van den Be, O Feron, B Ghesquière, H Prenen, C Libert, SR Walmsley, C Corbet, JA Van Ginder, M Ivan, D Taverna, M Mazzone
Science Advances, 2021;7(19):.
Species: Mouse
Sample Types: Plasma
-
Simvastatin Posttreatment Controls Inflammation and Improves Bacterial Clearance in Experimental Sepsis
Authors: FM de Jesus O, CF Gonçalves-, IMM de Moraes, PA Reis, VN Rocha, PT Bozza, AR Silva, HC de Castro
Mediators Inflamm, 2020;2020(0):1839762.
Species: Mouse
Sample Types: Cell Culture Supernates
-
Autophagy Inhibition Induces the Secretion of Macrophage Migration Inhibitory Factor (MIF) with Autocrine and Paracrine Effects on the Promotion of Malignancy in Breast Cancer
Authors: I Cotzomi-Or, A Rosas-Cruz, D Ramírez-Ra, J Reyes-Leyv, M Rodriguez-, P Aguilar-Al, P Maycotte
Biology (Basel), 2020;9(1):.
Species: Mouse
Sample Types: Cell Cultue Supernates
-
Involvement of HPV Infection in the Release of Macrophage Migration Inhibitory Factor in Head and Neck Squamous Cell Carcinoma
Authors: N Kindt, G Descamps, JR Lechien, M Remmelink, JM Colet, R Wattiez, G Berchem, F Journe, S Saussez
J Clin Med, 2019;8(1):.
Species: Mouse
Sample Types: Serum
-
Macrophage migration inhibitory factor enhances influenza-associated mortality in mice
Authors: CA Smith, DJ Tyrell, UA Kulkarni, S Wood, L Leng, RL Zemans, R Bucala, DR Goldstein
JCI Insight, 2019;4(13):.
Species: Mouse
Sample Types: BALF
-
Macrophage Migration Inhibitory Factor Promotes the Interaction between the Tumor, Macrophages, and T Cells to Regulate the Progression of Chemically Induced Colitis-Associated Colorectal Cancer
Authors: T Pacheco-Fe, I Juárez-Ave, O Illescas, LI Terrazas, R Hernández-, C Pérez-Plas, EB Gutiérrez-, F Ávila-More, YI Chirino, JL Reyes, V Maldonado, M Rodriguez-
Mediators Inflamm., 2019;2019(0):2056085.
Species: Mouse
Sample Types: Serum
-
Proinflammatory cytokine MIF plays a role in the pathogenesis of type-2 diabetes mellitus, but does not affect hepatic mitochondrial function
Authors: M Rodriguez-, T Cabellos-A, Y Sanchez-Za, I Juárez-Ave, E García-Rey, A Lira-León, JDC Benítez-Fl, T Pacheco-Fe, M Hiriart, EB Gutiérrez-
Cytokine, 2017;0(0):.
Species: Mouse
Sample Types: Serum
-
MIF Inhibitor ISO-1 Protects Photoreceptors and Reduces Gliosis in Experimental Retinal Detachment
Authors: B Kim, R Kusibati, T Heisler-Ta, D Mantopoulo, J Ding, MH Abdel-Rahm, AR Satoskar, JP Godbout, SK Bhattachar, CM Cebulla
Sci Rep, 2017;7(1):14336.
Species: Mouse
Sample Types: Complex Sample Type
FAQs
No product specific FAQs exist for this product, however you may
View all ELISA FAQsDuoSet Ancillary Reagent Kits
Supplemental ELISA Products
Reviews for Mouse MIF DuoSet ELISA
There are currently no reviews for this product. Be the first to review Mouse MIF DuoSet ELISA and earn rewards!
Have you used Mouse MIF DuoSet ELISA?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
