Mouse MINA Antibody

  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse MINA in direct ELISAs and Western blots. In direct ELISAs, 80% cross-reactivity with recombinant human (rh)  MINA is observed and 40% cross-reactivity with rhMINA is observed in Western blots.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant mouse MINA
    Pro2-Gly192
    Accession # Q8CD15
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Mouse MINA by Western Blot. Western blot shows lysates of P19 mouse embryonal carcinoma cell line and beta TC‑6 mouse beta cell insulinoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse MINA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7266) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for MINA at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.2 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MINA

MINA (myc-induced nuclear antigen; also Mina53) is a 52-54 kDa member of both the MINA53/NO66 and Jumonji C family of proteins. It expression is associated with proliferating cells, and it has been found in cytoplasm, nucleus and nucleoli. MINA appears to be induced by c-myc, and synthesized by spermatogonia, occasional squamous epithelium, naïve T cells and select cancer cells. When expressed, MINA is reported to regulate expression of genes such as HGF, EGF-R and IL-4. It may exert its regulatory activity through an intrinsic demethylase function. Mouse MINA is 465 amino acids (aa) in length. It possesses one cupin (or enzyme-associated) region (aa 51-363) that contains a JmjC domain (aa 139-271). There are two potential isoform variants that contain either a 12 aa substitution for aa 145-465, or a 15 aa substitution for aa 228-465. Over aa 2-192, mouse MINA shares 92% and 82% aa sequence identity with rat and human MINA, respectively.

  • Long Name:
    Myc-induced Nuclear Antigen
  • Entrez Gene IDs:
    84864 (Human); 67014 (Mouse); 266670 (Rat)
  • Alternate Names:
    DKFZp762O1912; FLJ14393; MDIG; MDIGDKFZp762O1912; MINA; Mina53; MINA53mdig; mineral dust induced gene protein; Mineral dust-induced gene protein; MYC induced nuclear antigen; MYC-induced nuclear antigen; myc-induced nuclear antigen, 53 kDa; NO52; Nucleolar protein 52
Related Research Areas
Isotype Controls
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Secondary Antibodies
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Donkey Anti-Sheep IgG Biotinylated Antibody

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Sheep IgG (H+L) PE-conjugated Antibody

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Sheep IgG (H+L) APC-conjugated Antibody

Flow F0127  
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Sheep IgG VisUCyte HRP Polymer Antibody

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Donkey Anti-Sheep IgG (H+L) Fluorescein-conjugated Antibody

Flow F0125 1
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Donkey Anti-Sheep IgG (H+L) PerCP-conjugated Antibody

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