Key Product Details

Species Reactivity

Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all MINA Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse MINA
Pro2-Gly192
Accession # Q8CD15

Specificity

Detects mouse MINA in direct ELISAs and Western blots. In direct ELISAs, 80% cross-reactivity with recombinant human (rh)  MINA is observed and 40% cross-reactivity with rhMINA is observed in Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Mouse MINA Antibody

Detection of Mouse MINA antibody by Western Blot.

Detection of Mouse MINA by Western Blot.

Western blot shows lysates of P19 mouse embryonal carcinoma cell line and beta TC-6 mouse beta cell insulinoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse MINA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7266) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for MINA at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Mouse MINA Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: P19 mouse embryonal carcinoma cell line and beta TC‑6 mouse beta cell insulinoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: MINA

MINA (myc-induced nuclear antigen; also Mina53) is a 52-54 kDa member of both the MINA53/NO66 and Jumonji C family of proteins. It expression is associated with proliferating cells, and it has been found in cytoplasm, nucleus and nucleoli. MINA appears to be induced by c-myc, and synthesized by spermatogonia, occasional squamous epithelium, naïve T cells and select cancer cells. When expressed, MINA is reported to regulate expression of genes such as HGF, EGF-R and IL-4. It may exert its regulatory activity through an intrinsic demethylase function. Mouse MINA is 465 amino acids (aa) in length. It possesses one cupin (or enzyme-associated) region (aa 51-363) that contains a JmjC domain (aa 139-271). There are two potential isoform variants that contain either a 12 aa substitution for aa 145-465, or a 15 aa substitution for aa 228-465. Over aa 2-192, mouse MINA shares 92% and 82% aa sequence identity with rat and human MINA, respectively.

Long Name

Myc-induced Nuclear Antigen

Alternate Names

MDIG, Mina53, NO52

Entrez Gene IDs

84864 (Human); 67014 (Mouse); 266670 (Rat)

Gene Symbol

RIOX2

UniProt

Q8CD15 (Mouse)

Additional MINA Products

Product Documents for Mouse MINA Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse MINA Antibody

For research use only

Related Research Areas

Cancer Biomarkers

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