|MR1 in Rat Intestine. MR1 was detected in perfusion fixed frozen sections of rat intestine using Rat Anti-Mouse MR1 Monoclonal Antibody (Catalog # MAB8526) at 25 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to villi epithelial cells. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.|
The Major Histocompatibility Complex (MHC) class I-related gene, MR1, considered a non-classical MHC class IA gene which is encoded outside the MHC region. The MR1 is responsible for activation of Mucosal-associated Invariant T (MAIT) cells expressing semi-invariant T cell receptors in the presence of bacteria. MR1 is a highly evolutionary conserved protein with regard to alpha 1 and alpha 2 domains corresponding to the peptide-binding domains of classical MHC class I molecules which show about 90% amino acid identity between human and mouse. MR1 protein can associate with beta 2-microglobulin, which indicates that this is a typical class I heterodimer composed of a heavy and a light chain resembling the classical MHC class I molecules.
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