Mouse MR1 Antibody

  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse MR1 in direct ELISAs.
  • Source
    Monoclonal Rat IgG2A Clone # 909417
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse MR1
    Met1-Arg296
    Accession # Q8HWB0
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Immunohistochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry
MR1 in Rat Intestine. MR1 was detected in perfusion fixed frozen sections of rat intestine using Rat Anti-Mouse MR1 Monoclonal Antibody (Catalog # MAB8526) at 25 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to villi epithelial cells. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MR1

The Major Histocompatibility Complex (MHC) class I-related gene, MR1, considered a non-classical MHC class IA gene which is encoded outside the MHC region. The MR1 is responsible for activation of Mucosal-associated Invariant T (MAIT) cells expressing semi-invariant T cell receptors in the presence of bacteria. MR1 is a highly evolutionary conserved protein with regard to alpha 1 and alpha 2 domains corresponding to the peptide-binding domains of classical MHC class I molecules which show about 90% amino acid identity between human and mouse. MR1 protein can associate with beta 2-microglobulin, which indicates that this is a typical class I heterodimer composed of a heavy and a light chain resembling the classical MHC class I molecules.  

  • Long Name:
    Major Histocompatibility Complex, Class I-Related
  • Entrez Gene IDs:
    3140 (Human); 15064 (Mouse)
  • Alternate Names:
    Class I histocompatibility antigen-like protein; FLJ31593; H2ls; HLALS; major histocompatibility complex class I-related gene protein; major histocompatibility complex, class I-like sequence; major histocompatibility complex, class I-related; MHC class I-like antigen MR-1; MHC class I-related gene protein; MHC class-I related-gene protein; MR1
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