Detects mouse MSP/MST1 in direct ELISAs.
In direct ELISAs, no cross-reactivity with
recombinant human (rh) MSP alpha, rhMSP beta, rhproMSP, rhMSP, or recombinant
mouse HGF is observed.
Monoclonal Rat IgG2A Clone # 757320
Protein A or G purified from hybridoma culture supernatant
Chinese hamster ovary cell line CHO-derived recombinant mouse MSP/MST1 Met1-Glu716 Accession # P26928
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
In a functional ELISA, 10-30 ng/mL of this antibody will block 50% of the binding of 10 ng/mL of Recombinant Mouse MSP/MST1 (Catalog # 6244-MS) to immobilized Recombinant Mouse MSP R/Ron Fc Chimera (Catalog # 431-MS) coated at 0.5 μg/mL (100 µL/well).
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
MSP/MST1 in Mouse Liver. MSP/MST1 was detected in perfusion fixed frozen sections of mouse liver using Rat Anti-Mouse MSP/MST1 Monoclonal Antibody (Catalog # MAB6244) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to hepatocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
MSP (Macrophage stimulating protein 1; also hepatocyte growth factor-like protein) is an 80-95 kDa member of the peptidase S1 family of proteins. Although it is expressed principally by hepatocytes, it can also be induced in cells such as renal tubular epithelium. MSP has multiple targets, and as such, has multiple effects. It stimulates macrophage motility and phagocytosis, promotes keratinocyte and renal tubular cell proliferation, and depresses myeloid progenitor cell replication. Mouse proMSP is 698 amino acids (aa) in length. It contains one PAN (carbohydrate-binding) site (aa 19-105), four consecutive kringle domains (110-457) and an inactive peptidase S1 region (aa 489-714). An intrachain disulfide bond exists between Cys477 and Cys593 that becomes an interchain bond following protease cleavage between Arg488 and Val489. This creates a mature heterodimer containing a 45-57 kDa alpha -chain, and a 30-35 kDa beta -chain. Mouse proMSP shares 80% and 93% aa identity with human and rat proMSP, respectively.
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