Key Product Details

Species Reactivity

Mouse

Applications

Immunohistochemistry, Neutralization

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 757320
View all MSP/MST1 Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant mouse MSP/MST1
Met1-Glu716
Accession # P26928

Specificity

Detects mouse MSP/MST1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) MSP alpha, rhMSP beta, rhproMSP, rhMSP, or recombinant mouse HGF is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Mouse MSP/MST1 Antibody

MSP/MST1 antibody in Mouse Liver by Immunohistochemistry (IHC-Fr).

MSP/MST1 in Mouse Liver.

MSP/MST1 was detected in perfusion fixed frozen sections of mouse liver using Rat Anti-Mouse MSP/MST1 Monoclonal Antibody (Catalog # MAB6244) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to hepatocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Applications for Mouse MSP/MST1 Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Perfusion fixed frozen sections of mouse liver

Neutralization

In a functional ELISA, 10-30 ng/mL of this antibody will block 50% of the binding of 10 ng/mL of Recombinant Mouse MSP/MST1 (Catalog # 6244-MS) to immobilized Recombinant Mouse MSP R/Ron Fc Chimera (Catalog # 431-MS) coated at 0.5 μg/mL (100 µL/well).

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: MSP/MST1

MSP (Macrophage stimulating protein 1; also hepatocyte growth factor-like protein) is an 80-95 kDa member of the peptidase S1 family of proteins. Although it is expressed principally by hepatocytes, it can also be induced in cells such as renal tubular epithelium. MSP has multiple targets, and as such, has multiple effects. It stimulates macrophage motility and phagocytosis, promotes keratinocyte and renal tubular cell proliferation, and depresses myeloid progenitor cell replication. Mouse proMSP is 698 amino acids (aa) in length. It contains one PAN (carbohydrate-binding) site (aa 19-105), four consecutive kringle domains (110-457) and an inactive peptidase S1 region (aa 489-714). An intrachain disulfide bond exists between Cys477 and Cys593 that becomes an interchain bond following protease cleavage between Arg488 and Val489. This creates a mature heterodimer containing a 45-57 kDa alpha -chain, and a 30-35 kDa beta -chain. Mouse proMSP shares 80% and 93% aa identity with human and rat proMSP, respectively.

Long Name

Macrophage Stimulating Protein/Macrophage Stimulating 1 [Hepatocyte Growth Factor-like]

Alternate Names

HGFL, MST1, SF2

Entrez Gene IDs

4485 (Human); 15235 (Mouse); 24566 (Rat)

Gene Symbol

MST1

UniProt

P26928

Additional MSP/MST1 Products

Product Documents for Mouse MSP/MST1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse MSP/MST1 Antibody

For research use only

Related Research Areas

Inflammatory Mediators

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Protocols

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