Mouse Myeloperoxidase DuoSet ELISA

Catalog # Availability Size / Price Qty
DY3667
Ancillary Products Available
Mouse Myeloperoxidase / MPO ELISA Standard Curve
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Product Details
Procedure
Citations (37)
FAQs
Supplemental Products
Reviews (4)

Mouse Myeloperoxidase DuoSet ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Volume Required
100 µL
Sufficient Materials
For fifteen 96-well plates*
Specificity
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant mouse Myeloperoxidase/MPO. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Kit Content

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.

The components listed above may be purchased separately:

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or 0.05% Tween® 20 in PBS, pH 7.2-7.4

Reagent Diluent: (Catalog # DY995), or 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990)

Plate Sealers: ELISA Plate Sealers (Catalog # DY992)

Data Example

Mouse Myeloperoxidase / MPO ELISA Standard Curve

Product Datasheets

Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Myeloperoxidase/MPO

Myeloperoxidase (MPO) is a heme-containing enzyme belonging to the XPO subfamily of peroxidases. It is an abundant neutrophil and monocyte glycoprotein that catalyzes the hydrogen peroxide dependent formation of hypochlorus acid (HOCl) and other reactive species. Enzymatically active MPO is a disulfide-linked tetramer that contains two heme groups and two copies each of the heavy and light chains. MPO binds Albumin, MMR, Cytokeratin 1 on vascular endothelial cells, HMW Kininogen, and Integrin CD11b/CD18 on neutrophils. These interactions promote MPO clearance, a reduction of nitric oxide and bradykinin levels, reduced vasodilation, and continued neutrophil activation. Neutrophil MPO is stored in cytoplasmic azurophilic granules. Upon cellular activation and degranulation, MPO is delivered into phagosomes where it is required for the killing of phagocytosed bacteria. Activated neutrophils also release granule contents extracellularly. Elevated plasma MPO levels have been associated with a variety of clinical conditions including systemic inflammation, eclampsia, risk of cardiovascular events, vascular endothelial dysfunction, severity of multiple sclerosis, and prospective mortality and oxidative stress during hemodialysis.

Entrez Gene IDs:
4353 (Human); 17523 (Mouse); 303413 (Rat)
Alternate Names:
EC 1.11.1; EC 1.11.1.7; Lactoperoxidase; MPO; Myeloperoxidase

Assay Procedure

GENERAL ELISA PROTOCOL

Plate Preparation

  1. Dilute the Capture Antibody to the working concentration in PBS without carrier protein. Immediately coat a 96-well microplate with 100 μL per well of the diluted Capture Antibody. Seal the plate and incubate overnight at room temperature.
  2. Aspirate each well and wash with Wash Buffer, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (400 μL) using a squirt bottle, manifold dispenser, or autowasher. Complete removal of liquid at each step is essential for good performance. After the last wash, remove any remaining Wash Buffer by aspirating or by inverting the plate and blotting it against clean paper towels.
  3. Block plates by adding 300 μL Reagent Diluent to each well. Incubate at room temperature for a minimum of 1 hour.
  4. Repeat the aspiration/wash as in step 2. The plates are now ready for sample addition.

Assay Procedure

  1. Add 100 μL of sample or standards in Reagent Diluent, or an appropriate diluent, per well. Cover with an adhesive strip and incubate 2 hours at room temperature.
  2. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  3. Add 100 μL of the Detection Antibody, diluted in Reagent Diluent, to each well. Cover with a new adhesive strip and incubate 2 hours at room temperature.
  4. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  5. Add 100 μL of the working dilution of Streptavidin-HRP to each well. Cover the plate and incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  6. Repeat the aspiration/wash as in step 2.
  7. Add 100 μL of Substrate Solution to each well. Incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  8. Add 50 μL of Stop Solution to each well. Gently tap the plate to ensure thorough mixing.
  9. Determine the optical density of each well immediately, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. If wavelength correction is not available, subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.

Citations for Mouse Myeloperoxidase DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

37 Citations: Showing 1 - 10
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  1. Evidence of CD40L/CD40 pathway involvement in experimental transfusion-related acute lung injury
    Authors: S Tariket, H Hamzeh-Cog, S Laradi, CA Arthaud, MA Eyraud, T Bourlet, P Berthelot, O Garraud, F Cognasse
    Sci Rep, 2019;9(1):12536.
    Species: Mouse
    Sample Types: BALF
  2. Gut microbiota from high-risk men who have sex with men drive immune activation in gnotobiotic mice and in vitro HIV infection
    Authors: SX Li, S Sen, JM Schneider, KN Xiong, NM Nusbacher, N Moreno-Hui, M Shaffer, AJS Armstrong, E Severs, K Kuhn, CP Neff, M McCarter, T Campbell, CA Lozupone, BE Palmer
    PLoS Pathog., 2019;15(4):e1007611.
    Species: Mouse
    Sample Types: Cell Lysates
  3. GPER activation protects against epithelial barrier disruption by Staphylococcus aureus ?-toxin
    Authors: KD Triplett, S Pokhrel, MJ Castleman, SM Daly, BO Elmore, JA Joyner, G Sharma, G Herbert, MJ Campen, HJ Hathaway, ER Prossnitz, PR Hall
    Sci Rep, 2019;9(1):1343.
    Species: Mouse
    Sample Types: Tissue Homogenates
  4. PAD4-dependent NETs generation are indispensable for intestinal clearance of Citrobacter rodentium
    Authors: P Saha, BS Yeoh, X Xiao, RM Golonka, V Singh, Y Wang, M Vijay-Kuma
    Mucosal Immunol, 2019;0(0):.
    Species: Mouse
    Sample Types: Serum
  5. Long noncoding RNA Malat1 regulates differential activation of macrophage and response to lung injury
    Authors: H Cui, S Banerjee, S Guo, N Xie, J Ge, D Jiang, M Zörnig, VJ Thannickal, G Liu
    JCI Insight, 2019;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  6. Comparison of livestock-associated and community-associated Staphylococcus aureus pathogenicity in a mouse model of skin and soft tissue infection
    Authors: PR Randad, CA Dillen, RV Ortines, D Mohr, M Aziz, LB Price, H Kaya, J Larsen, KC Carroll, TC Smith, LS Miller, CD Heaney
    Sci Rep, 2019;9(1):6774.
    Species: Mouse
    Sample Types: Tissue Homogenates
  7. Complement factor C5 inhibition reduces type 2 responses without affecting group 2 innate lymphoid cells in a house dust mite induced murine asthma model
    Authors: J Yang, I Ramirez Mo, C van 't Vee, AF de Vos, R de Beer, JJTH Roelofs, BP Morgan, T van der Po
    Respir. Res., 2019;20(1):165.
    Species: Mouse
    Sample Types: BALF
  8. Mesenchymal Stromal Cells Inhibit Neutrophil Effector Functions in a Murine Model of Ocular Inflammation
    Authors: SK Mittal, A Mashaghi, A Amouzegar, M Li, W Foulsham, SK Sahu, SK Chauhan
    Invest. Ophthalmol. Vis. Sci., 2018;59(3):1191-1198.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  9. Identification and Complete Stereochemical Assignments of the New Resolvin Conjugates in Tissue Regeneration in Human Tissues that Stimulate Proresolving Phagocyte Functions and Tissue Regeneration
    Authors: X de la Rosa, PC Norris, N Chiang, AR Rodriguez, BW Spur, CN Serhan
    Am. J. Pathol., 2018;188(4):950-966.
    Species: Mouse
    Sample Types: Tissue Homogenates
  10. Myeloperoxidase deficiency attenuates systemic and dietary iron-induced adverse effects
    Authors: X Xiao, P Saha, BS Yeoh, JA Hipp, V Singh, M Vijay-Kuma
    J. Nutr. Biochem., 2018;62(0):28-34.
    Species: Mouse
    Sample Types: Tissue Homogenates
  11. Myocardial Ischemia Reperfusion Injury: Apoptotic, Inflammatory and Oxidative Stress Role of Galectin-3
    Authors: S Al-Salam, S Hashmi
    Cell. Physiol. Biochem., 2018;50(3):1123-1139.
    Species: Mouse
    Sample Types: Tissue Homogenates
  12. Metformin relieves acute respiratory distress syndrome by reducing miR-138 expression
    Authors: LL Yu, M Zhu, Y Huang, YM Zhao, JJ Wen, XJ Yang, P Wu
    Eur Rev Med Pharmacol Sci, 2018;22(16):5355-5363.
    Species: Mouse
    Sample Types: Tissue Homogenates
  13. Exosomal microRNA predicts and protects against severe bronchopulmonary dysplasia in extremely premature infants
    Authors: CV Lal, N Olave, C Travers, G Rezonzew, K Dolma, A Simpson, B Halloran, Z Aghai, P Das, N Sharma, X Xu, K Genschmer, D Russell, T Szul, N Yi, JE Blalock, A Gaggar, V Bhandari, N Ambalavana
    JCI Insight, 2018;3(5):.
    Species: Mouse
    Sample Types: BALF
  14. Interleukin-22-deficiency and microbiota contribute to the exacerbation of Toxoplasma gondii-induced intestinal inflammation
    Authors: A Couturier-, N Froux, J Piotet-Mor, C Michaudel, L Brault, J Le Bériche, A Sénéchal, P Robinet, P Chenuet, S Jejou, L Dumoutier, JC Renauld, J Iovanna, S Huber, V Quesniaux, H Sokol, B Ryffel
    Mucosal Immunol, 2018;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  15. Lack of Histamine H4-Receptor Expression Aggravates TNBS-Induced Acute Colitis Symptoms in Mice
    Authors: EJ Wunschel, B Schirmer, R Seifert, D Neumann
    Front Pharmacol, 2017;8(0):642.
    Species: Mouse
    Sample Types: Tissue Homogenates
  16. Innate Sex Bias of Staphylococcus aureus Skin Infection Is Driven by ?-Hemolysin
    Authors: MJ Castleman, S Pokhrel, KD Triplett, DF Kusewitt, BO Elmore, JA Joyner, JK Femling, G Sharma, HJ Hathaway, ER Prossnitz, PR Hall
    J. Immunol., 2017;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  17. Cross-modulation of pathogen-specific pathways enhances malnutrition during enteric co-infection with Giardia lamblia and enteroaggregative Escherichia coli
    Authors: LA Bartelt, DT Bolick, J Mayneris-P, GL Kolling, GL Medlock, EI Zaenker, J Donowitz, RV Thomas-Bec, A Rogala, IM Carroll, SM Singer, J Papin, JR Swann, RL Guerrant
    PLoS Pathog., 2017;13(7):e1006471.
    Species: Mouse
    Sample Types: Feces
  18. Protective effects of ethyl pyruvate on lipopolysaccharide?induced acute lung injury through inhibition of autophagy in neutrophils
    Authors: Q Zhu, H Wang, H Wang, Y Luo, Y Yu, Q Du, A Fei, S Pan
    Mol Med Rep, 2017;15(3):1272-1278.
    Species: Mouse
    Sample Types: BALF
  19. Recombinant human IL-26 facilitates the innate immune response to endotoxin in the bronchoalveolar space of mice in vivo
    Authors: A Bao, KF Che, S Bozinovski, J Ji, JA Gregory, S Kumlien Ge, M Adner, LO Cardell, A Lindén
    PLoS ONE, 2017;12(12):e0188909.
    Species: Mouse
    Sample Types: BALF
  20. Dietary Salt Exacerbates Experimental Colitis
    Authors: AL Tubbs, B Liu, TD Rogers, RB Sartor, EA Miao
    J. Immunol., 2017;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  21. Corneal stromal stem cells reduce corneal scarring by mediating neutrophil infiltration after wounding
    Authors: AJ Hertsenber, G Shojaati, ML Funderburg, MM Mann, Y Du, JL Funderburg
    PLoS ONE, 2017;12(3):e0171712.
    Species: Mouse
    Sample Types: Whole Tissue
  22. Experimental atopic dermatitis depends on IL-33R signaling via MyD88 in dendritic cells
    Authors: C Li, I Maillet, C Mackowiak, C Viala, F Di Padova, M Li, D Togbe, V Quesniaux, Y Lai, B Ryffel
    Cell Death Dis, 2017;8(4):e2735.
    Species: Mouse
    Sample Types: Tissue Homogenates
  23. Bacterial Siderophores Hijack Neutrophil Functions
    Authors: P Saha, BS Yeoh, RA Olvera, X Xiao, V Singh, D Awasthi, BC Subramania, Q Chen, M Dikshit, Y Wang, CA Parent, M Vijay-Kuma
    J. Immunol., 2017;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  24. Isothiocyanate-enriched moringa seed extract alleviates ulcerative colitis symptoms in mice
    Authors: Y Kim, AG Wu, A Jaja-Chime, BL Graf, C Waterman, MP Verzi, I Raskin
    PLoS ONE, 2017;12(9):e0184709.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  25. Pterostilbene 4'-?-Glucoside Protects against DSS-Induced Colitis via Induction of Tristetraprolin
    Authors: Y Chen, J Park, Y Joe, HJ Park, SJ Jekal, D Sato, H Hamada, HT Chung
    Oxid Med Cell Longev, 2017;2017(0):9427583.
    Species: Mouse
    Sample Types: Tissue Homogenates
  26. Contribution of the Ade Resistance-Nodulation-Cell Division-Type Efflux Pumps to Fitness and Pathogenesis of Acinetobacter baumannii
    Authors: Eun-Jeong Yoon
    MBio, 2016;7(3):.
    Species: Mouse
    Sample Types: BALF
  27. IL-1R1-MyD88 axis elicits papain-induced lung inflammation
    Eur J Immunol, 2016;0(0):.
    Species: Mouse
    Sample Types: BALF
  28. A�a� Berries Inhibit Colon Tumorigenesis in Azoxymethane/Dextran Sulfate Sodium-Treated Mice
    Authors: Yoon Jin Choi
    Gut Liver, 2016;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  29. Glaucocalyxin A improves survival in bleomycin-induced pulmonary fibrosis in mice
    Authors: Li Zhu
    Biochem. Biophys. Res. Commun., 2016;0(0):.
    Species: Mouse
    Sample Types: BALF
  30. Nox2-derived oxidative stress results in inefficacy of antibiotics against post-influenza S. aureus pneumonia
    Authors: K Sun, VK Yajjala, C Bauer, GA Talmon, KJ Fischer, T Kielian, DW Metzger
    J. Exp. Med, 2016;213(9):1851-64.
    Species: Mouse
    Sample Types: Tissue Homogenates
  31. Effects of a Soluble Epoxide Hydrolase Inhibitor on Lipopolysaccharide-Induced Acute Lung Injury in Mice
    PLoS ONE, 2016;11(8):e0160359.
    Species: Mouse
    Sample Types: Tissue Homogenates
  32. Identification of resolvin D2 receptor mediating resolution of infections and organ protection.
    Authors: Chiang N, Dalli J, Colas R, Serhan C
    J Exp Med, 2015;212(8):1203-17.
    Species: Mouse
    Sample Types: Tissue Homogenates
  33. CD36 Is Essential for Regulation of the Host Innate Response to Staphylococcus aureus alpha-Toxin-Mediated Dermonecrosis.
    Authors: Castleman M, Febbraio M, Hall P
    J Immunol, 2015;195(5):2294-302.
    Species: Mouse
    Sample Types: Tissue Homogenates
  34. Tristetraprolin mediates anti-inflammatory effect of carbon monoxide against DSS-induced colitis.
    Authors: Joe Y, Uddin M, Zheng M, Kim H, Chen Y, Yoon N, Cho G, Park J, Chung H
    PLoS ONE, 2014;9(2):e88776.
    Species: Mouse
    Sample Types: Tissue Homogenates
  35. Allergic airway inflammation decreases lung bacterial burden following acute Klebsiella pneumoniae infection in a neutrophil- and CCL8-dependent manner.
    Authors: Dulek D, Newcomb D, Goleniewska K, Cephus J, Zhou W, Reiss S, Toki S, Ye F, Zaynagetdinov R, Sherrill T, Blackwell T, Moore M, Boyd K, Kolls J, Peebles R
    Infect Immun, 2014;82(9):3723-39.
    Species: Mouse
    Sample Types: Tissue Homogenates
  36. Resolvin D1 stimulates alveolar fluid clearance through alveolar epithelial sodium channel, Na,K-ATPase via ALX/cAMP/PI3K pathway in lipopolysaccharide-induced acute lung injury.
    Authors: Wang Q, Zheng X, Cheng Y, Zhang Y, Wen H, Tao Z, Li H, Hao Y, Gao Y, Yang L, Smith F, Huang C, Jin S
    J Immunol, 2014;192(8):3765-77.
    Species: Rat
    Sample Types: Tissue Homogenates
  37. Limited role of nuclear receptor Nur77 in Escherichia coli-induced peritonitis.
    Authors: Hamers A, Uleman S, van Tiel C, Kruijswijk D, van Stalborch A, Huveneers S, de Vries C, van 't Veer C
    Infect Immun, 2014;82(1):253-64.
    Species: Mouse
    Sample Types: Tissue Homogenates

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Reviews for Mouse Myeloperoxidase DuoSet ELISA

Average Rating: 4.3 (Based on 4 Reviews)

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Mouse Myeloperoxidase DuoSet ELISA
By Anonymous on 06/08/2017
Sample Tested: Lung tissue

Mouse Myeloperoxidase DuoSet ELISA
By Anonymous on 06/08/2017
Sample Tested: Lung tissue

Mouse Myeloperoxidase DuoSet ELISA
By Anonymous on 04/21/2017
Sample Tested: Colon tissue

Mouse Myeloperoxidase DuoSet ELISA
By Anonymous on 06/02/2016
Sample Tested: Adult lung

The data is yet to be published and hence cannot give my graph.

Will give my data once it is published.