Mouse Nectin-2/CD112 Antibody

Catalog # Availability Size / Price Qty
AF3869
AF3869-SP
Detection of Mouse Nectin‑2/CD112 by Western Blot.
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Mouse Nectin-2/CD112 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Nectin-2/CD112 in Western blots.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Nectin-2/CD112
Gln32-Gly351
Accession # P32507
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse Nectin-2/CD112 antibody by Western Blot. View Larger

Detection of Mouse Nectin‑2/CD112 by Western Blot. Western blot shows lysates of C2C12 mouse myoblast cell line and NIH-3T3 mouse embryonic fibroblast cell line. PVDF Membrane was probed with 1 µg/mL of Mouse Nectin-2/CD112 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3869) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Nectin-2/CD112 at approximately 65-70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Nectin-2/CD112

Nectins are a small family of Ca++-independent immunoglobulin (Ig)-like cell adhesion molecules (CAMs) that control cell adhesion, proliferation, and migration (1, 2, 3). The name Nectin derives from the Latin word necto, which means “to connect”. The Nectin family contains four members (Nectin-1 - 4), all of which show alternate splicing, a transmembrane (TM) region (except for Nectin-1 gamma  which is secreted), and three extracellular Ig-domains. Nectins are highly homologous to the human receptor for poliovirus, and as such have been given the alternate name of poliovirus receptor-related proteins. They do not, however, appear to bind poliovirus (1). Mouse Nectin-2 is a 70 to 78 kDa type I TM glycoprotein that is found on a variety of cell types (4, 5). It has two splice forms (4, 6, 7). Nectin-2 alpha /PRR2 is a 65 kDa short form and is synthesized as a 467 amino acid precursor. It contains a 31 aa signal sequence, a 315 aa extracellular domain (ECD), a 28 aa TM segment, and a 93 aa cytoplasmic region. The ECD contains one N-terminal V-type Ig domain and two 85-95 aa C2-type Ig-like domains (aa 153-337) (8). The V-domain is believed to mediate Nectin binding to its ligands (9). A long, 78 kDa, 530 aa isoform of mouse Nectin-2 (Nectin-2δ) also exists. It has the same signal sequence and extracellular domain as Nectin-2 alpha  (aa 1-338), but differs in the TM segment (21 aa in length) and cytoplasmic region (159 aa in length) (4, 6, 7). Mouse Nectin-2 ECD (aa 32-338) shares 72%, 77% and 95% aa identity with the ECD in human, canine and rat Nectin-2, respectively. Nectin-2 is known to bind pseudorabies virus, and herpes simplex virus-2 (HSV-2). It also binds select HSV-1 strains. It does not bind poliovirus (1, 10, 11). As a cell adhesion molecule, Nectin-2 will form cis-homodimers (same cell) and trans-homodimers (across cells). Nectin-2 will not cis-dimerize with other Nectins, but will trans-heterodimerize with Nectin-3 and CD266/DNAM-1 (1, 3, 11, 12, 13). Nectin-2 is found concentrated at cell-to-cell interfaces, and is presumed to contribute to tight and adherens junction formation (14). Through its interaction with NK and T cell expressed DNAM-1, it also promotes lymphocyte cytotoxicity and cytokine secretion against both tumors and dendritic cells (DC) expressing Nectin-2 (15, 16). In the case of DC, this may be a mechanism whereby the immune system eliminates DC that are inefficient at antigen presentation. Nectin-2 is expressed on epithelium, endothelial cells, Sertoli cells, monocytes, dendritic cells, granulosa cells, mast cells, eosinophils and fibroblasts.

References
  1. Takai, Y. and H. Nakanishi (2003) J. Cell Sci. 116:17.
  2. Rikitake, Y. and Y. Takai (2008) Cell. Mol. Life Sci. 65:253.
  3. Sasisaka, T. et al. (2007) Curr. Opin. Cell Biol. 19:1.
  4. Aoki, J. et al. (1994) J. Biol. Chem. 269:8431.
  5. Takahashi, K. et al. (1999) J. Cell Biol. 145:539.
  6. Aoki, J. et al. (1997) Exp. Cell Res. 235:374.
  7. Lopez, M. et al. (1998) Blood 92:4602.
  8. Morrison, M.E. and V.R. Racaniello (1992) J. Virol. 66:2807.
  9. Struyf, F. et al. (2002) J. Virol. 76:12940.
  10. Delboy, M.G. et al. (2006) Virology J. 3:105.
  11. Irie, K. et al. (2004) Semin. Cell Dev. Biol. 15:643.
  12. Tahara-Hanaoka, S. et al. (2004) Int. Immunol. 16:533.
  13. Satoh-Horikawa, K. et al. (2000) J. Biol. Chem. 275:10291.
  14. Nakanishi, H. and Y. Takai (2004) Biol. Chem. 385:885.
  15. Tahara-Hanaoka, S. et al. (2006) Blood 107:1491.
  16. Pende, D. et al. (2006) Blood 107:2030.
Long Name
Poliovirus Receptor Related 2
Entrez Gene IDs
5819 (Human); 19294 (Mouse); 102137456 (Cynomolgus Monkey)
Alternate Names
CD112 antigen; CD112; Herpes virus entry mediator B; Herpesvirus entry mediator B; herpesvirus entry protein B; HVEB; HVEBpoliovirus receptor-like 2; MPH; nectin 2; Nectin2; Nectin-2; poliovirus receptor-related 2 (herpesvirus entry mediator B); poliovirus receptor-related protein 2; PRR2; PRR2nectin-2; PVRL2; PVRR2poliovirus receptor related 2

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