Mouse Neuroplastin Antibody

Catalog # Availability Size / Price Qty
AF7818
AF7818-SP
Mouse Neuroplastin Antibody in Data
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Product Details
Citations (2)
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Mouse Neuroplastin Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Neuroplastin in direct ELISAs and Western blots. In direct ELISAs, approximately 100% cross-reactivity with recombinant human Neuroplastin-65 is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Neuroplastin
Gln29-Pro338, predicted
Accession # P97300
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.05 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Mouse Neuroplastin antibody by Western Blot. View Larger

Detection of Mouse Neuroplastin by Western Blot. Western blot shows lysates of mouse brain (whole) tissue and mouse brain (cerebellum) tissue. PVDF membrane was probed with 0.05 µg/mL of Sheep Anti-Mouse Neuroplastin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7818) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Neuroplastin at approximately 55-65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Neuroplastin

Neuroplastin (NPTN or neural plasticity-dependent synaptic rearrangement protein; also Np65 and SDR-1) is a 63-67 kDa member of the CD147 family, Ig‑superfamily of molecules. Its expression is primarily restricted to the PSD of select neurons, and it likely serves as a cell adhesion molecule between cells. NPTN is known to bind homotypically in trans, and this complex appears to interact with FGFR1 in cis. Engagement of the FGFR induces neurite outgrowth. Notably, rodent and human Np65 show distinctly different staining patterns in the hippocampus and cerebellum of the brain. Mature mouse NPTN is a 369 amino acid (aa) type I transmembrane glycoprotein. It possesses a 310 aa extracellular region (aa 29-338) that contains three Ig-like domains (aa 29-134; 148-234; 237-327), and a 38 aa cytoplasmic tail. There are three splice forms that have been described. One is a widely-expressed 53-57 kDa isoform (Np55) that contains a Glu substitution for aa 31‑147, a second is a splice form that shows the above substitution coupled to a deletion of aa 371-374, and a third is an isoform that contains a nine aa substitution for aa 1-235. Over aa 1-338, mouse NPTN shares 95% and 98% aa sequence identity with human and rat NPTN/Np65, respectively.

Entrez Gene IDs
27020 (Human); 20320 (Mouse); 56064 (Rat)
Alternate Names
AW554172; GP55; GP55Stromal cell-derived receptor 1; GP65SDR-1; GP66; MGC102805; Neuroplastin; np55; np65; NPTN; SDFR1; SDFR1DKFZp686L2477; SDR1; SDR1stromal cell derived factor receptor 1

Product Datasheets

Citations for Mouse Neuroplastin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Trans-differentiation of outer hair cells into inner hair cells in the absence of INSM1
    Authors: T Wiwatpanit, SM Lorenzen, JA Cantú, CZ Foo, AK Hogan, F Márquez, JC Clancy, MJ Schipma, MA Cheatham, A Duggan, J García-Año
    Nature, 2018;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. Neuroplastin Isoform Np55 Is Expressed in the Stereocilia of Outer Hair Cells and Required for Normal Outer Hair Cell Function
    J Neurosci, 2016;36(35):9201-16.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr

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