Mouse Neutrophil Elastase/ELA2 Antibody

Catalog # Availability Size / Price Qty
MAB4517
MAB4517-SP
Detection of Mouse Neutrophil Elastase/ELA2 by Western Blot.
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Product Details
Citations (5)
FAQs
Supplemental Products
Reviews (1)

Mouse Neutrophil Elastase/ELA2 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Neutrophil Elastase/ELA2 in direct ELISAs and Western blots.
Source
Monoclonal Rat IgG2A Clone # 887105
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Neutrophil Elastase/ELA2
Met1-Arg260
Accession # Q3UP87
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse Neutrophil Elastase/ELA2 antibody by Western Blot. View Larger

Detection of Mouse Neutrophil Elastase/ELA2 by Western Blot. Western blot shows lysates of mouse bone marrow. PVDF membrane was probed with 0.5 µg/mL of Rat Anti-Mouse Neutrophil Elastase/ELA2 Monoclonal Antibody (Catalog # MAB4517) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Neutrophil Elastase/ELA2 at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Neutrophil Elastase/ELA2

Neutrophil Elastase (ELA2), also known as Leukocyte Elastase, Bone marrow serine protease, Medullasin and ELANE, is a 25-30 kDa serine protease belonging to the peptidase S1 family. ELA2 is secreted by neutrophils during inflammation, it destroys bacteria and host tissue. ELA2 function is the degradation of many extracellular matrix macromolecules. ELA2 is localized to keratohyalin granules, where it was found to directly participate in pro-filaggrin processing.  When expressed aberrantly, ELA2 can cause emphysema or emphysematous changes, it involves breakdown of the lung structure and increased airspaces. Alpha-1 Antitrypsin (Serpin A1) and secretory leukocyte protease inhibitor (SLPI) have been shown to inhibit ELA2 activity.

Entrez Gene IDs
1991 (Human); 50701 (Mouse)
Alternate Names
Bone marrow serine protease; EC 3.4.21; EC 3.4.21.37; ELA2; ELA2granulocyte-derived elastase; ELANE; elastase 2, neutrophil; elastase, neutrophil expressed; Elastase-2; GE; HLEelastase-2; HNE; Human leukocyte elastase; Leukocyte Elastase; Medullasin; NE; Neutrophil Elastase; PMN elastase; PMN-E; polymorphonuclear elastase; SCN1

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Citations for Mouse Neutrophil Elastase/ELA2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. S100A9 is indispensable for survival of pneumococcal pneumonia in mice
    Authors: Ostermann, L;Seeliger, B;David, S;Flasche, C;Maus, R;Reinboth, MS;Christmann, M;Neumann, K;Brand, K;Seltmann, S;Bühling, F;Paton, JC;Roth, J;Vogl, T;Viemann, D;Welte, T;Maus, UA;
    PLoS pathogens
    Species: Mouse
    Sample Types: BALF
    Applications: Western Blot
  2. Neutrophil Extracellular Traps Mediate Acute Liver Failure in Regulation of miR-223/neutrophil Elastase Signaling in Mice
    Authors: D Ye, J Yao, W Du, C Chen, Y Yang, K Yan, J Li, Y Xu, S Zang, Y Zhang, X Rong, R Zhang, A Xu, J Guo
    Cellular and Molecular Gastroenterology and Hepatology, 2022-06-02;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates, Whole Cells, Whole Tissue
    Applications: ICC, IHC, Western Blot
  3. A novel in vivo model for extracellular vesicle-induced emphysema
    Authors: C Margaroli, MC Madison, L Viera, DW Russell, A Gaggar, KR Genschmer, JE Blalock
    JCI Insight, 2022-01-25;0(0):.
    Species: Mouse
    Sample Types: Extracellular Vesicles
    Applications: Flow Cytometry
  4. IL-1? activation in response to Staphylococcus aureus lung infection requires inflammasome-dependent and independent mechanisms
    Authors: S Pires, D Parker
    Eur. J. Immunol., 2018-08-19;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  5. Midkine drives cardiac inflammation by promoting neutrophil trafficking and NETosis in myocarditis
    Authors: Ludwig T. Weckbach, Ulrich Grabmaier, Andreas Uhl, Sebastian Gess, Felicitas Boehm, Annette Zehrer et al.
    Journal of Experimental Medicine

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Reviews for Mouse Neutrophil Elastase/ELA2 Antibody

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Mouse Neutrophil Elastase/ELA2 Antibody
By Anonymous on 07/21/2017
Application: WB Sample Tested: Kidney tissue Species: Mouse

- loaded 10, 20, 30ug of mouse kidney tissue
- blocked and diluted ELA2 Ab in 5% milk (1:1000)
- not sure what the HMW bands is though