Detection of Mouse Neutrophil Elastase/ELA2 by Western Blot.
Western blot shows lysates of mouse bone marrow. PVDF membrane was probed with 0.1 µg/mL of Sheep Anti-Mouse Neutrophil Elastase/ELA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4517) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Neutrophil Elastase/ELA2 at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Neutrophil Elastase/ELA2
Neutrophil Elastase (ELA2), also known as Leukocyte Elastase, Bone marrow serine protease, Medullasin and ELANE, is a 25-30 kDa serine protease belonging to the peptidase S1 family. ELA2 is secreted by neutrophils during inflammation, it destroys bacteria and host tissue. ELA2 function is the degradation of many extracellular matrix macromolecules. ELA2 is localized to keratohyalin granules, where it was found to directly participate in pro-filaggrin processing. When expressed aberrantly, ELA2 can cause emphysema or emphysematous changes, it involves breakdown of the lung structure and increased airspaces. Alpha-1 Antitrypsin (Serpin A1) and secretory leukocyte protease inhibitor (SLPI) have been shown to inhibit ELA2 activity.
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