Mouse NG2/MCSP Antibody

Catalog # Availability Size / Price Qty
MAB6689
MAB6689-SP
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NG2/MCSP in Mouse Brain.
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Product Details
Citations (8)
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Reviews (1)

Mouse NG2/MCSP Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse NG2/MCSP in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human NG2/MCSP/CSPG4 is observed.
Source
Monoclonal Rat IgG1 Clone # 546930
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse NG2/MCSP
Leu1587-Ser2229
Accession # Q8VHY0
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Immunohistochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry NG2/MCSP antibody in Mouse Brain by Immunohistochemistry (IHC-Fr). View Larger

NG2/MCSP in Mouse Brain. NG2/MCSP was detected in perfusion fixed frozen sections of mouse brain (cortex) using Rat Anti-Mouse NG2/MCSP Monoclonal Antibody (Catalog # MAB6689) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hemotoxylin (blue). Specific staining was localized to glial cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NG2/MCSP

NG2, also called MCSP (melanoma-associated chondroitin sulfate proteoglycan), CSPG4, or AN2, is a 400-500 kDa proteoglycan found on multiple cell types, including immature oligodendrocytes, osteoblasts, chondroblasts, and pericytes.  It interacts with integrin alpha 3 beta 1, galectin 3, MMP16, collagen VI and PDGFR alpha.  Mature mouse NG2 is a 2298 amino acid (aa) type I transmembrane glycoprotein.  The extracellular region is 2200 aa in length (aa 30-2229), while the cytoplasmic domain is 76 aa in size. The glycoprotein component of NG2 is approximately 300-315 kDa in size.  Proteolytic cleavage generates a 290 and 275 kDa form (excluding the chondroitin sulfate moiety).  There are two splice variants.  One shows a 38 aa substitution for aa 1657-2327, while another shows an alternate start site at Met1667.  Over aa 31-420, mouse NG2 shares 95% and 80% aa identity with rat and human NG2, respectively.    

Long Name
Chondroitin Sulfate Proteoglycan NG2/Melanoma Associated Chondroitin Sulfate Proteoglycan
Entrez Gene IDs
1464 (Human); 121021 (Mouse); 81651 (Rat)
Alternate Names
AN2; chondroitin sulfate proteoglycan 4 (melanoma-associated); chondroitin sulfate proteoglycan 4; CSPG4; EC 3.6.3; HMW-MAA; MCSP; MCSPChondroitin sulfate proteoglycan NG2; MCSPG; MCSPGMelanoma chondroitin sulfate proteoglycan; MEL-CSPG; MSK16Melanoma-associated chondroitin sulfate proteoglycan; NG2; NG2EC 2.7.8

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Citations for Mouse NG2/MCSP Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. 20-?Hydroxycholesterol, an oxysterol in human breast milk, reverses mouse neonatal white matter injury through Gli-dependent oligodendrogenesis
    Authors: Chao, AS;Matak, P;Pegram, K;Powers, J;Hutson, C;Jo, R;Dubois, L;Thompson, JW;Smith, PB;Jain, V;Liu, C;Younge, NE;Rikard, B;Reyes, EY;Shinohara, ML;Gregory, SG;Goldberg, RN;Benner, EJ;
    Cell stem cell
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. NO-sensitive guanylyl cyclase discriminates pericyte-derived interstitial from intra-alveolar myofibroblasts in murine pulmonary fibrosis
    Authors: Aue, A;Englert, N;Harrer, L;Schwiering, F;Gaab, A;König, P;Adams, R;Schmidtko, A;Friebe, A;Groneberg, D;
    Respiratory research
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. Active immunotherapy reduces NOTCH3 deposition in brain capillaries in a CADASIL mouse model
    Authors: DV Oliveira, KG Coupland, W Shao, S Jin, F Del Gaudio, S Wang, R Fox, JW Rutten, J Sandin, H Zetterberg, J Lundkvist, SA Lesnik Obe, U Lendahl, H Karlström
    Embo Molecular Medicine, 2022-12-16;0(0):e16556.
    Species: Mouse
    Sample Types: Tissue Homeogenates
    Applications: IHC
  4. PDGFRbeta+ cells play a dual role as hematopoietic precursors and niche cells during mouse ontogeny
    Authors: D Sá da Band, AM Kilpatrick, M Marques, M Gomez-Sala, T Ventura, ZN Gonzalez, D Stefancova, F Rossi, M Vermeren, CS Vink, M Beltran, NC Henderson, B Jung, R van der Li, HJG van de Wer, WFJ van Ijcken, C Betsholtz, SJ Forbes, H Cuervo, M Crisan
    Oncogene, 2022-07-19;40(3):111114.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  5. Deficiency of Notch signaling in pericytes results in arteriovenous malformations
    Authors: T Nadeem, W Bogue, B Bigit, H Cuervo
    JCI Insight, 2020-11-05;5(21):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  6. Dysregulated mesenchymal PDGFR-&beta drives kidney fibrosis
    Authors: EM Buhl, S Djudjaj, BM Klinkhamme, K Ermert, VG Puelles, MT Lindenmeye, CD Cohen, C He, E Borkham-Ka, R Weiskirche, B Denecke, P Trairatphi, J Saez-Rodri, TB Huber, LE Olson, J Floege, P Boor
    EMBO Mol Med, 2020-01-14;0(0):e11021.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  7. Metformin inhibits metastatic breast cancer progression and improves chemosensitivity by inducing vessel normalization via PDGF-B downregulation
    Authors: JC Wang, GY Li, B Wang, SX Han, X Sun, YN Jiang, YW Shen, C Zhou, J Feng, SY Lu, JL Liu, MD Wang, PJ Liu
    J. Exp. Clin. Cancer Res., 2019-06-04;38(1):235.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  8. Silencing of galectin-1 inhibits retinal neovascularization and ameliorates retinal hypoxia in a murine model of oxygen-induced ischemic retinopathy
    Authors: N Yang, W Zhang, T He, Y Xing
    Exp. Eye Res, 2017-02-28;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC

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Mouse NG2/MCSP Antibody
By Anonymous on 11/02/2016
Application: Immunocytochemistry/Immunofluorescence Sample Tested: Brain tissue Species: Mouse