Mouse NG2/MCSP Antibody

(1 Review)
  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse NG2/MCSP in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human NG2/MCSP/CSPG4 is observed.
  • Source
    Monoclonal Rat IgG1 Clone # 546930
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse NG2/MCSP
    Leu1587-Ser2229
    Accession # Q8VHY0
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Immunohistochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry
NG2/MCSP in Mouse Brain. NG2/MCSP was detected in perfusion fixed frozen sections of mouse brain (cortex) using Rat Anti-Mouse NG2/MCSP Monoclonal Antibody (Catalog # MAB6689) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hemotoxylin (blue). Specific staining was localized to glial cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: NG2/MCSP
NG2, also called MCSP (melanoma-associated chondroitin sulfate proteoglycan), CSPG4, or AN2, is a 400-500 kDa proteoglycan found on multiple cell types, including immature oligodendrocytes, osteoblasts, chondroblasts, and pericytes.  It interacts with integrin alpha 3 beta 1, galectin 3, MMP16, collagen VI and PDGFR alpha.  Mature mouse NG2 is a 2298 amino acid (aa) type I transmembrane glycoprotein.  The extracellular region is 2200 aa in length (aa 30-2229), while the cytoplasmic domain is 76 aa in size. The glycoprotein component of NG2 is approximately 300-315 kDa in size.  Proteolytic cleavage generates a 290 and 275 kDa form (excluding the chondroitin sulfate moiety).  There are two splice variants.  One shows a 38 aa substitution for aa 1657-2327, while another shows an alternate start site at Met1667.  Over aa 31-420, mouse NG2 shares 95% and 80% aa identity with rat and human NG2, respectively.    
  • Long Name:
    Chondroitin Sulfate Proteoglycan NG2/Melanoma Associated Chondroitin Sulfate Proteoglycan
  • Entrez Gene IDs:
    1464 (Human); 121021 (Mouse); 81651 (Rat)
  • Alternate Names:
    AN2; chondroitin sulfate proteoglycan 4 (melanoma-associated); chondroitin sulfate proteoglycan 4; CSPG4; EC 3.6.3; HMW-MAA; MCSP; MCSPChondroitin sulfate proteoglycan NG2; MCSPG; MCSPGMelanoma chondroitin sulfate proteoglycan; MEL-CSPG; MSK16Melanoma-associated chondroitin sulfate proteoglycan; NG2; NG2EC 2.7.8
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Average Rating: 4 (Based on 1 review)

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We have 1 review tested in 1 species: Mouse.
We have 1 review tested in 1 application: Immunocytochemistry/Immunofluorescence.

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Immunocytochemistry/Immunofluorescence Mouse NG2/MCSP Antibody MAB6689
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Very Good
 ICC/IF Mouse Anonymous 11/02/2016
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Immunocytochemistry/Immunofluorescence Mouse NG2/MCSP Antibody MAB6689
Immunocytochemistry/Immunofluorescence: Mouse NG2/MCSP Antibody [MAB6689]

Summary

ApplicationImmunocytochemistry/Immunofluorescence
Sample TestedBrain tissue
SpeciesMouse

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