Detection of OSM R beta in D3 Mouse Cell Line by Flow Cytometry.
D3 mouse embryonic stem cell line was stained with Rat Anti-Mouse OSM R beta Biotinylated Monoclonal Antibody (Catalog # BAM662, filled histogram) or isotype control antibody (Catalog # IC006B, open histogram), followed by Streptavidin-Phycoerythrin (Catalog # F0040).
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: OSM R beta
Oncostatin M (OSM) is a member of the IL-6 family of cytokines that share the gp130 as a common signal transducing receptor subunit. Human OSM signals through two types of human OSM receptor complexes: the type I complex comprising the Leukemia Inhibitory Factor Receptor beta (LIF R beta ) and gp130, the type II complex made up of OSM Receptor beta (OSM R beta ) and gp130. In contrast, mouse OSM signals only through the mouse OSM R beta and gp130 complex. Mouse OSM R beta cDNA encodes a 971 amino acid (aa) residue type I transmembrane protein which contains a 23 aa residue signal peptide, an extracellular domain of 714 aa, a transmembrane domain of 20 aa and a 214 aa cytoplasmic domain. Mouse OSM R beta alone binds mOSM with low-affinity, but forms a high-affinity binding complex in the presence of gp130. Mouse OSM R beta is 55% identical at the amino acid sequence level to human OSM R beta.
Lindberg, R.A. et al. (1998) Mol. Cell. Biol. 18:3357.
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